Talk:Sex-determining region Y protein

BASEDbio (talk) 20:06, 7 October 2013 (UTC) Heykei1 (talk) 01:56, 8 October 2013 (UTC)Pitpeelorchard (talk) 10:36, 9 October 2013 (UTC)

Wiki Education Foundation-supported course assignment

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Suggestion

It would be helpful if you could elaborate on how the SRY gene gets expressed, leading to the production of TDF, as well as the type of signaling pathway that TDF is involved in (specifically leading to primary sex cord development). It gets a little confusing/misleading in the second paragraph with the use of the words gene and protein; "This gene is a DNA-binding protein that enhances other transcription factors, or is a transcription factor itself." It would be helpful if you could clarify that the gene (SRY) codes for a protein (TDF) which then leads to the transcription of the primary sex cords mRNA by some signaling pathway. BASEDbio (talk) 23:30, 3 October 2013 (UTC)

Merger proposal

Sorry for butting in here just before the start of this course assignment. But I think it would worthwhile to discuss this merger proposal before rather than after this assignment starts.

This article is about the Testis determining factor protein while the SRY article is about the gene encoding this protein. Because the subject of the gene and the protein are so interrelated, I think it makes more sense to have one article that discusses both. For same reason, the vast majority of the other ~10,000 Gene Wiki articles are about both the protein and the gene encoding that protein. Hence I think these two articles should be merged. Thoughts? Boghog (talk) 20:29, 9 October 2013 (UTC)

BASEDbio has made some helpful suggestions above about reducing the confusion between the gene and protein. I think this might be easier if the two articles were merged. In addition, a merger would eliminate the inevitable overlap that is created by having two separate articles. Boghog (talk) 20:39, 9 October 2013 (UTC)

We completely agree! We will be focusing on both SRY and TDF as one topic/article. An outline will be posted on the talk page shortly. BASEDbio (talk) 23:10, 17 October 2013 (UTC)

Bibliography of Relevant Research

Philippe B, Hawkins RJ, Sinclair AH, Taylor A, Griffiths BL, Goodfellow PN, and Fellous M. “Genetic evidence equating SRY and the testis-determining factor.” Human Molecular Genetics, Imperial Cancer Research Fund, Lincoln’s Inn Fields, London, UK. Nature: Vol. 348, 29 November 1990. <http://www.bio.davidson.edu/Courses/Molbio/restricted/01GenSRY/1990_evidence.pdf>

Harley VR, Clarkson MJ, Argentaro A. The molecular action and regulation of the testis-determining factors, SRY (sex-determining region on the Y chromosome) and SOX9 [SRY-related high-mobility group (HMG) box 9]. Prince Henry's Institute of Medical Research, Victoria, Australia. 2003 Aug: 24(4):466-87. NCBI. PubMed. <http://www.ncbi.nlm.nih.gov/pubmed/12920151>

“Sex Determining Region Y.” GeneCards. The Human Gene Compendium. <http://www.genecards.org/cgi-bin/carddisp.pl?gene=SRY>

“SRY_HUMAN.” Reviewed, UniProtKB/Swiss-Prot. Uniprot. <http://www.uniprot.org/uniprot/Q05066#section_ref>

Nia2993 (talk) 02:18, 15 October 2013 (UTC)

Nishino K,Hattori N, Tanaka S, and Shiota K. "DNA Methylation-mediated Control of Sry Gene Expression in Mouse Gonadal Development." The Journal of Biological Chemistry, 21 May 2004, 279, 22306-22313. <http://www.jbc.org/content/279/21/22306.short%20--%3E%20methylation%20of%20SRY%20gene%20in%20mice#cited-by>

N.A Hanley, D.M Hagan, M Clement-Jones, S.G Ball, T Strachan, L Salas-Cortés, K McElreavey, S Lindsay, S Robson, P Bullen, H Ostrer, D.I Wilson, SRY, SOX9, and DAX1 expression patterns during human sex determination and gonadal development, Mechanisms of Development, Volume 91, Issues 1–2, 1 March 2000, Pages 403-407, ISSN 0925-4773. <http://www.sciencedirect.com/science/article/pii/S092547739900307X>

Cunha J.L., Soardi F.C., Bernardi R.D., Oliveira L.E.C., Benedetti C.E., Guerra-Junior G., Maciel-Guerra A.T., and de Mello M.P. "The novel p.E89K mutation in the SRY gene inhibits DNA binding and causes the 46,XY disorder of sex development." Brazilian Journal of Medical and Biological Research, 2011 April: 43 (4):268-80. <http://www.scielo.br/pdf/bjmbr/v44n4/817.pdf >

Heykei1 (talk) 03:03, 15 October 2013 (UTC)

Sekido R, Lovell-Badge R. "Genetic control of testis development." Division of Stem Cell Biology and Developmental Genetics, MRC National Institute for Medical Research, London, 5 september 2012: <http://www.ncbi.nlm.nih.gov/pubmed/22964823>

Ryan NA, Akbar S. "A case report of an incidental finding of a 46,XX, SRY-negative male with masculine phenotype during standard fertility workup with review of the literature and proposed immediate and long-term management guidance." Luton and Dunstable National Health Service Foundation Trust, Department of Obstetrics and Gynaecology, London, United Kingdom. 3 January 2013: <http://www.ncbi.nlm.nih.gov/pubmed/23290744>

Itman C, Loveland KL."Smads and cell fate: distinct roles in specification, development, and tumorigenesis in the testis." Department of Biochemistry & Molecular Biology, Monash University, Clayton, Victoria, Australia. 9 January 2013: <http://www.ncbi.nlm.nih.gov/pubmed/23300154>

BASEDbio (talk) 04:08, 15 October 2013 (UTC)

Summary of Article to Come

The article will include both TDF and SRY as one topic (see "merger proposal" for reasons). A section devoted to the SRY gene will detail the region of DNA that SRY is found on (the Y chromosome), the transcription/regulatory factors that control expression of the SRY gene, the signaling pathways that these factors use to activate SRY, and any post-transcriptional modifications leading to the mRNA that codes for TDF. The next section will focus on Testes Determining Factor (the SRY protein). Emphasis will be placed on the function of TDF as a transcription factor. This will include the signaling pathway(s) it is involved in, other transcription factors that it interacts with, and the genes that these transcription factors regulate. The next section will describe how the activation of these genes (by TDF and its associated transcription factors) result in maleness/testes formation. This will include a description of the proteins these genes code for and how they ultimately lead to the development of testes/the male sex organs. The final section will briefly discuss the disorders that result from a malfunction in this extensive signaling pathway. — Preceding unsigned comment added by BASEDbio (talk • contribs) 04:56, 18 October 2013 (UTC)

Member Contribution

Responsibility for the development of the Gene Regulation section. Pitpeelorchard (talk) 19:02, 18 November 2013 (UTC)

Peer Article Review

Overall the article is really great! There is a lot of good information and all the major points seemed to be covered thoroughly. You also have a lot of wiki-links to other articles, which was really helpful. I found myself using some of them from time to time to make sure I knew exactly what was going on. One suggestion I have is for the flow of the introduction. When I was reading the first paragraph I was questioning about SRY and wished I had known more about that before delving into the TDF. Maybe conjoining the two paragraphs or adding a short introduction to SRY first might be helpful to the flow, rather than explaining TDF and then going back and explaining SRY, since there is no link to a separate article for SRY. Also, you have an image for SRY, but is there an image for TDF specifically? That might be helpful as well. I really liked how you set up your next three sections. I thought that the flowed well into each other. I liked how you started out by telling the audience that SRY is hard to study and slightly complicated, however I was curious about what types of mutations cause sex reversal at the end of that paragraph. Is there a way you could link to a wiki article on sex reversal, or find out anything about these mutations to just add a quick line about it? In the second gene regulation paragraph, I had a few questions about the genes and binding site names. I know that WT1 has a wiki-link, but are there wiki-links to the other two, SP1 and SF1? I found myself getting a little lost through that section and could use a little more explanation about the SF1, SP1, and WT1 elements. What exactly are they? What do they stand for? Also in that paragraph, I thought the part about DAX regulation seemed rushed. Is there any more information on that? How does DAX interact with this process, since it hasn’t been talked about in the previous sections before? The evolution paragraph was great but I almost wish it was early in the article, maybe in the introduction or before gene regulation? That helped me put it into perspective and if you leave it there that’s fine, but personally I think it would have helped me earlier. The introduction to the Function section was perfect in my opinion. It made sense. It flowed well. And it had links when I needed them. I just noticed that there weren’t any references cited in this paragraph and I don’t know if that was your paragraph or one that came with the article but it might be a good idea to add some references if you can. In Action in the nucleus, the sentences flowed well and the information wasn’t too overwhelming. I think this was also a good paragraph. I just have a question about NLS and what it is, but that’s very minor. Again, your SOX9 paragraphs flowed well. There were a few bits of information that I wished I had known earlier, like the SF1 protein information and function, but overall I didn’t find too many problems with these paragraphs. I thought your function sections were very well done. The Sex Influences sections were also well done. I really liked how you included the different syndromes and mutations with the SRY and sex development. I think the title of the next part may improve if you change it slightly. After reading the paragraph before it about the different syndromes, I thought I was going to be reading more of the same and was surprised to see that there was talk of schizophrenia and Parkinson’s. I’m not exactly sure what you should change it to if you do decide to do that, but it might make it clearer to make it a big more specific to your paragraph. And the Olympic screening was very interesting! Were there specific cases of this that you could link in? I found myself wanting to read more into the situation and learn about athletes that were found to be SRY-positive. If you could add more about that, I think it would be a nice addition. So, overall, your article is very well done. I thought you did a really good job getting the major points and you referenced well. I think you could use a few more pictures if you can – maybe in the last part with the different disease or the athletes (maybe a picture of an athlete who couldn’t compete if that’s possible to find?) – and finding up some flow issues. Once you fix the flow, I think your article will be superb and it clearly shows you’ve put a lot of work into it already. Great job! T1d7 (talk) 17:55, 22 November 2013 (UTC)

This a very thorough article! I can tell that you did a lot of research and drew from many sources. I thought the article flowed really well. Starting with a definition and its basic roles allows the reader to get a general idea before the more complex sections come later. One suggestion I have is for you to come up with a definition of TDF that can be stated in one sentence, and make that your first sentence. This would allow the reader to get a good grasp quickly without having to read the whole first paragraph. In addition, you could use your first section as a way to briefly introduce some of the other topics you address later in the article. The Regulation section is very thorough in that it explains the difficulties that arose in trying to study the gene. I do think that the Evolution section may be better as its own section, however, and not as a subset of Gene Expression. The Influence on Sex part was very interesting to me as it provided an example of how the TDF works at a scale larger than the molecular level. It involves some of the issues that can arise when TDF doesn't work as it should or is lacking, which were easy to understand and more relatable than other sections (which were necessary too, of course). The section on Olympic Testing was very interesting as well; it was something that I had not heard about before. Do you know if there is something used currently to supplement the testing that was eliminated? Overall, the article was very well-written. I made a few minor grammatical changes to the articles, but nothing too big. You linked your article well with other articles, making it easy for a reader to get to a page when they don't understand a term you used. You also did a good job overall at using language that is easy for the common reader to know, and explaining things that could not be out into plain language. It appears as if your citations were made correctly, and your Further Reading section can help someone access good articles to further their research. I really enjoyed this article; the amount of work you put in clearly shows! Allielew00 (talk) 21:02, 22 November 2013 (UTC)

Overall, the Wikipedia page for Testes determining factor (TDF) is very thorough and very interesting! There are a few things you might want to consider doing to make sure that your article is as great as it can be. I think it would be helpful to create an entirely separate section for the specifics of the SRY gene, and another separate section for the specifics of the TDF protein. I think the second paragraph in the introduction can be removed from the introduction, and divided between these two new separate sections. It would be helpful to include more specifics for the SRY gene. For example, instead of just saying that it is on Chromosome Y, you could say that it is on chromosome Y at base pair position 2,654,896-2,655,792. It might also be helpful to include that the gene is 897 base pairs long (these specifics are for humans, but it would be nice to include other mammals too; perhaps you could include a table of the specifics for each species). The source for this information is the UCSC genome browser: http://genome.ucsc.edu/cgi-bin/hgTracks?position=chrY:2654896-2655792&hgsid=353663593&knownGene=pack&hgFind.matches=uc004fqg.1, It would also be nice to see specifics like this for the TDF protein. For example, how many base pairs long is the TDF protein? Is it found in all cells, or only some cells? I think that the “gene regulation” portion of the your page can be re-named Gene history/evolution, because it doesn’t really talk about gene regulation at all, it mostly focuses on the Gene history and evolution. Additionally, in the beginning paragraph, you say that TDF influences other transcription factors, but you don’t say what those other transcription factors are. As a reader, I was really curious about the specifics of those other transcription factors. On a similar note, you also say that some animals lack this gene entirely and use an alternative form of sex differentiation in the “evolution” section. For those animals, what alternative form of sex differentiation do they use? Just a few wiki links to other pages that describe these alternative forms of sex differentiation would be really nice. In the section "Influence on sex" you say “Crossing over during paternal meiosis prior to conception can cause SRY to be transferred from the Y chromosome to the X chromosome. The Y chromosome, now lacking an SRY gene, can no longer initiate testis development.” These sentences occur in two separate paragraphs, but they are directly related to each other. Instead of starting a new paragraph with “The Y chromosome”, you should start it with “crossing over during paternal..” With the current paragraph break, it’s really confusing that these two sentences are discussing the same phenomenon. Lastly, the section on Swyer syndrome is a little confusing. Why do both male and female organs develop? I think you are suggesting that X-inactivation leads to different genes on the X chromosome being active in different cells, but I’m not sure. It would be helpful if you clarified this, and maybe provided some background about X-inactivation and barr bodies. I really enjoyed reading your article, and I can't wait to see where you take it from here! I hope this review was helpful, let me know if you have any questions about it! Blairwal (talk) 22:03, 22 November 2013 (UTC)

Overall, your work here is impressive. This is a great improvement from how it was before (I was considering this as a choice at the beginning of the course) and the wikilines really helped with filling me in on info that I might have needed to check up on. Some helpful suggestions might be as follows. You could definitely talk about the SRY gene separately from TDF since it does provide the factor being talked about here and could help in increasing the flow of the page. You would first talk about the gene, and then the product, simple as that. Also, make sure to define TDF as simple as possible, within a sentence maybe so you can create the hook that will bring the reader deep within the page. And obviously you all have done that for the rest of the page. I think anyone could understand this page with a little time put into reading it in it's entirety. The section on Diseases and Defects seems to be lacking. You have the names, but you might want to add more info on the basics of how these problems stem from SRY. And I must say that the last section on the Olympics was very interesting. It might cause the reader to switch to researching more about the social aspect of it, but it is the end of the page so there you go. One final piece would have to be to change the name of the page to SRY since you clearly like to use that name better than TDF. Just switch it around in the beginning and you should be good. Well, thats it, if you have any questions, be sure to ask here or in class. Other than that, I really enjoyed reading your page and congratulate you all on a job well done! E9P2 (talk) 05:08, 25 November 2013 (UTC)

Response to Peer Reviews

Olympic testing: added additional information about the testing currently used for gender identification and more specifics of athletes that were found to have the SRY gene (it seems specific names of athletes are not available for privacy reasons) BASEDbio (talk) 22:19, 6 December 2013 (UTC)

Introduction: combined the (previously) 2 paragraphs into one and eliminated redundancies/simplified the material for better flow. Also specified the transcription factors TDF upregulates (mainly SOX9) and mentioned SF1 and its role with TDF, as suggested (so it doesn't come out of nowhere in later sections) BASEDbio (talk) 00:03, 7 December 2013 (UTC) I chose to make one paragraph (like the first review suggested) for ease of reading/better flow. I did not make a separate paragraph for SRY (as the last couple reviews mentioned) because the bulk of the page/info is on TDF, which is the same as SRY.p so there is little to say about SRY, other than what is mentioned in the intro. I did explain it first though, so it flows better into the introduction of TDF (as was suggested). I also started with a single sentence definition of TDF (suggested by several of the reviews) to make the intro easier to understand, and mentioned more of the topics discussed in later sections.BASEDbio (talk) 00:12, 7 December 2013 (UTC)

Gene Evolution and Regulation: as suggested this section was reformatted to talk about evolution first, regulation second. More details about the transcription factors Sp1, SF1 and WT1 and specifics about how they function in regulation SRY were added as suggested by T1d7. More details about DAX1 and why its interaction with SRY are problematic in developing a straightforward understanding of SRY regulation. A few more details were added about evidence of GATA4 and FOG2 regulation of SRY. In general these edits were in response to several reviewers asking us to clarify the details on how SRY is regulated at the promoter. Pitpeelorchard (talk) 20:24, 9 December 2013 (UTC)

Action in the nucleus: added specifics about the TDF protein, including some basic structure and sequence information. Also, the use of the abbreviation SF1 was clarified but giving the full name and linking to the article describing the protein Nia2993 (talk) 20:23, 7 December 2013 (UTC)

Influence on Sex: Expanded and clarified the information about X-inactivation in XX male syndrome. Also reformatted the paragraph break between the first and second paragraph for better flow. Changes the title of the subsection Diseases and Defects to Role in Other Diseases, due to some confusion as to what that meant. Heykei1 (talk) 20:44, 7 December 2013 (UTC)

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Wiki Education Foundation-supported course assignment

This article was the subject of a Wiki Education Foundation-supported course assignment, between 23 January 2019 and 17 May 2019. Further details are available on the course page. Student editor(s): Miyeak, Cluffa, Halleripon. Peer reviewers: Oimoenm, Mindiolam, Vohraa.

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Wiki Education Foundation-supported course assignment

This article was the subject of a Wiki Education Foundation-supported course assignment, between 24 March 2020 and 29 April 2020. Further details are available on the course page. Student editor(s): Alexis Skipper. Peer reviewers: Boucherdh, MDDominguez91.

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Requested move 15 November 2016

The following is a closed discussion of a requested move. Please do not modify it. Subsequent comments should be made in a new section on the talk page. Editors desiring to contest the closing decision should consider a move review. No further edits should be made to this section.

The result of the move request was: Moved. No opposition, and although it has never actually been moved, so "wrong previous move" is not warranted, the assertion that common name includes the comma is reasonable.  — Amakuru (talk) 09:42, 24 November 2016 (UTC)

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Testis determining factor → Testis-determining factor – Seems like a wrong previous move - Page itself uses hyphen as do External IDs and other refs including PMID Iztwoz (talk) 17:37, 15 November 2016 (UTC)

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The above discussion is preserved as an archive of a requested move. Please do not modify it. Subsequent comments should be made in a new section on this talk page or in a move review. No further edits should be made to this section.

Classwork

Hey, I've chosen this article to edit for a biology class. It looks like it's been a while since anyone edited the talk page, but I thought I would ask if there are any edits the community thinks need to be made? Otherwise, sources permitting, I would like to edit the sections about evolution, the role in the nucleus, and the role in other diseases. Thanks for any advice! Cluffa (talk) 21:59, 27 February 2019 (UTC)

Peer Review

This Wiki page seems pretty thorough already, so adding new parts to sections would be what I would focus on. There has to be more information out there for the weaker sections. The Evolution of the SRY gene could be a part to focus on since there seems to be a lack of information. There is probably studies you could find if you searched for the evolution of it. That would be useful information to have. Continuing on things you could add to, the part near the end talking about ongoing research could use help. Maybe adding new research to that area to explain what is specifically being looked at could be good information. — Preceding unsigned comment added by Oimoenm (talk • contribs) 20:13, 31 March 2019 (UTC)

Peer Review

Overall the article is thorough and has enough pertinent information on the topic for people to gain a general understanding of the testis determining factor. Some things that may need to be touched up is linking some of the forms of analysis like microarray and chromatin immunoprecipitation to the appropriate wiki pages. The last paragraph may need some more added to it if there is more out there, and the second sentence needs a citation attached to it I think. Those fixes are small, and I think otherwise the article is solid. It has a nice lead section and has most of the appropriate links and citations as well. Some more information could be added to the ongoing research section or possibly the role in other diseases, but I like the section on the olympic screening as it remained objective and gave a nice overview of the history of a semi-controversial topic. Mindiolam (talk) 19:28, 7 April 2019 (UTC)

Aafreen's Peer Review

This article overall does a well job of explaining what the testis determining factor (TDF) is. It goes into detail on how the process of TDF works to create testis through transcription and upregulation. Throughout this article, include some more information and sources about the evolution of the SRY gene because I would personally like to know more about it. Although the section about the SRY evolution is not too long, the information you do have is useful but I think there could be some more. It's really interesting to know that the SRY gene has been shown to have come from the original Y chromosome in humans and that it is only one of four genes on the Y chromosome. For this study, using mice was plausible since they are mammals and easy subjects to study and they reproduce easily. Overall this article has plenty of information but it could use a few edits. In the regulation section, include links to certain terms that may not be understood by the average person coming across this article. Also during the Action in the nucleus section include some links as well for terms such as nuclear localization, importin β, calmodulin, Amh gene, and prostaglandin D synthase (Ptgds) gene. In general, this article has a lot of useful information but could use some more in certain areas, especially after having links to certain terms. There is plenty of information to be understood so adding more sections that go into detail about these would be helpful. The lead section of the article is great and gives detailed information about how the testis determining factor works. There is a clear structure among this article but it could be improved by adding more sections. There is a balance between each section, it is broken down nicely into three sections with subsections under each. I believe that they are in the right order of importance when pertaining to the TDF. The content and the way it is written is in a neutral point of view. There is no overarching viewpoint over the other and the authors did a good job of keeping the paper neutral. The article does have plenty of reliable sources with useful information to further improve this article. Overall this article was an interesting read and I learned valuable information that will be useful throughout this class. Although there are some small grammar errors and not having links to certain terminology, this is a well written article. Vohraa (talk) 06:11, 10 April 2019 (UTC) Aafreen Vohra Vohraa (talk) 17:34, 10 April 2019 (UTC)Aafreen

Hatnote added

I think many will land at SRY looking for information about sex differentiation in general, perhaps believing that SRY is the only piece of the puzzle. So I have added a hatnote: "For a broader background, see Sexual differentiation in humans."

I hope y'all are okay with this, but revert me if I was wrong to add an unusual type of hatnote. Jruderman (talk) 23:02, 1 August 2024 (UTC)

Image is too pretty

This glowy illustration in the infobox looks great, but some parts of it are hard to see, as if they're in shadow. Should we use a different one? #A11Y Jruderman (talk) 23:10, 1 August 2024 (UTC)

I've asked @The Cheesedealer:, who created and uploaded the image, to weigh in. Jruderman (talk) 23:13, 1 August 2024 (UTC)

Was thinking the same actually but been lazy to re-render, imma do that tonight. I no longer have the model so the re-render won't be the same, I hope y'all fine with that — 🧀The Cheesedealer ^squeak!⚟ 23:17, 1 August 2024 (UTC)

@Jruderman we happy? — 🧀The Cheesedealer ^squeak!⚟ 13:36, 2 August 2024 (UTC)

Thanks, Cheesedealer. The new image is a little better, but it's still hard for me to see the dark parts on my computer screen. Is that an RNA molecule that it's binding with, or part of the SRY protein itself? I'd appreciate if you were to try again with different colors or lighting. A caption might also be in order. Jruderman (talk) 15:54, 2 August 2024 (UTC)