Wikipedia talk:USEP/Courses/JHU MolBio Ogg SP14/Group 82F

Initial article assessments from Klbarnhill

Alpha operon ribosome binding site

This article is rated stub class, as it is very short and can be confusing to the average reader. The mechanism implies that translations produces four products, one of which acts as a repressor that turns off translation^[1]. The encoded protein that acts as the repressor recognizes the initiation sequence on the RNA and binds to it, preventing further translation of the mRNA^[2]

Capping Enzyme

Also a stub class article, very short. RNA caps are positively charged bits of nucleic acid that are attached to the 5' end of growing RNA shortly after the initiation of transcription^[3] . It has been found that the enzyme responsible for the 5' capping is comprised of two separate chains^[4]

References

^ Schlax, PJ (October 19, 2001). "Translational repression of the Escherichia coli alpha operon mRNA: importance of an mRNA conformational switch and a ternary entrapment complex". The Journal of Biological Chemistry. PMID 11504736. Retrieved 25 February 2014. {{cite journal}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)
^ Tang, Careen K (May 19, 1989). "Unusual mRNA pseudoknot structure is recognized by a protein translational repressor". Cell. 57 (4): 531–536. doi:10.1016/0092-8674(89)90123-2. Retrieved 25 February 2014. {{cite journal}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)
^ Cho, Eun-Jung (1997). "mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polymerase II carboxy-terminal domain". Genes and Development (11). doi:10.1101/gad.11.24.3319. Retrieved 25 February 2014. {{cite journal}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)
^ Shibagaki, Yoshio (August 30, 1991). "mRNA Capping Enzyme" (PDF). The Journal of Biological Chemistry. 14. 267 (May): 9521–9528. Retrieved 25 February 2014. {{cite journal}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)

Group page

Hi Katherine, Yes, this is our group page. I believe we have to post our assessments under the Project page.SabFernMB (talk) 02:42, 26 February 2014 (UTC)[reply]

Awesome, I think I did it correctly :) Have you done the article assessments?? Klbarnhill (talk) 03:08, 26 February 2014 (UTC)[reply]

Yes, I posted it on the Project Page Tab.SabFernMB (talk) 03:18, 26 February 2014 (UTC)[reply]

Article Selection

Hi Katherine, Do you have a preference on the article you want to work on? I saw that we both assessed the capping enzymes article. We can work on this or pick another article. What do you think of reviewing all our options articles and making a decision by Thursday? SabFernMB (talk) 03:16, 26 February 2014 (UTC)[reply]

Hi Katherine, after researching a couple of additional articles, I liked the following: glycine riboswitch and capping enzymes. Both cover an aspect of gene expression, which I find very interesting. Glycine riboswitch regulates the expression of downstream gene, while capping enzymes are involved in mRNA processing. I don’t know a lot about either topic, and working on either article would be a great way to learn more about the RNA element or enzyme. Let me know if either article interests you or if you prefer to work on another article. I am not tied to either article and I am open to other topics. Thanks SabFernMB (talk) 03:38, 27 February 2014 (UTC)[reply]

Hi Katherine Have you had a chance to look over the articles? We have to pick up an article by tomorrow as well as provide a small summary on why we selected it. Please let me know what you would like to work on. Thanks.SabFernMB (talk) 01:48, 4 March 2014 (UTC)[reply]

Hey--I'm so sorry, I haven't received emails that you had posted here! Since we both looked at the capping enzyme article, let's do that one! Klbarnhill (talk) 20:48, 4 March 2014 (UTC)[reply]

Also, I went ahead and claimed Capping Enzymes, I will compose a summary and post it, feel free to go in and edit/add! Klbarnhill (talk) 20:54, 4 March 2014 (UTC)[reply]

Hi Katherine, No problem! Thanks for claiming the article and starting the summary. It's a great start! We have to have 200-300 words so I will add to it. SabFernMB (talk) 00:02, 5 March 2014 (UTC)[reply]

Hi Katherine, I modified the summary to include additional points and to make it flow. Would you please do the final review to make sure it is okay? Please feel free to edit it.SabFernMB (talk) 01:55, 5 March 2014 (UTC)[reply]

Transfer of references to a sandbox

Hi Katherine, I hope you are okay with using my sandbox for this week's assignment. I transferred our references to my sandbox as it so that we can build upon them as per this week's assignment.SabFernMB (talk) 03:31, 10 March 2014 (UTC)[reply]

Sounds good to me! Klbarnhill (talk) 20:45, 10 March 2014 (UTC)[reply]

Preliminary Outline

Hi Katherine, I drafted an outline for the article and posted it on my sandbox. Please feel free to edit/add to it. I found a couple of references and images. The images of the enzyme structure were obtained from Wiki commons. I hoping we could an image that illustrates the process. Do you know of other sites we can check for free images? SabFernMB (talk) 00:10, 12 March 2014 (UTC)[reply]

I know of a protein database that has 3D structures, I'll search for proteins involved! Klbarnhill (talk) 00:30, 12 March 2014 (UTC)[reply]

I've added some additional images and description in the outline. Klbarnhill (talk) 00:58, 12 March 2014 (UTC)[reply]

That's great! SabFernMB (talk) 01:07, 12 March 2014 (UTC)[reply]

Progress report

Please review the report on the Group page and feel free to edit it. Thanks. SabFernMB (talk) 02:50, 26 March 2014 (UTC)[reply]

[Schlax-1] Schlax, PJ (October 19, 2001). "Translational repression of the Escherichia coli alpha operon mRNA: importance of an mRNA conformational switch and a ternary entrapment complex". The Journal of Biological Chemistry. PMID 11504736. Retrieved 25 February 2014. {{cite journal}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)

[Tang-2] Tang, Careen K (May 19, 1989). "Unusual mRNA pseudoknot structure is recognized by a protein translational repressor". Cell. 57 (4): 531–536. doi:10.1016/0092-8674(89)90123-2. Retrieved 25 February 2014. {{cite journal}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)

[Cho-3] Cho, Eun-Jung (1997). "mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polymerase II carboxy-terminal domain". Genes and Development (11). doi:10.1101/gad.11.24.3319. Retrieved 25 February 2014. {{cite journal}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)

[4] Shibagaki, Yoshio (August 30, 1991). "mRNA Capping Enzyme" (PDF). The Journal of Biological Chemistry. 14. 267 (May): 9521–9528. Retrieved 25 February 2014. {{cite journal}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)

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