User:HlynurT/Discovery and development of thalidomide and its analogs
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Introduction
| Thalidomide | |
|---|---|
| Drug class | |
 Thalidomide, the parent drug of thalidomide analogs. | |
| Class identifiers | |
| ATC code | L04AX |
| Biological target | Immunosuppressant |
| Legal status | |
Thalidomide once marketed as a antiemetic and sedative drug in the late 1950s was banned in 1961 after it‘s teratogenic properties were observed. However thalidomide was later found out to be both antiangiogenetic and anti-inflammatory yielding a new way of fighting number of immunological and inflammatory diseases. Recently, thalidomide has been approved by the Food and Drug Administration (FDA) for use in newly diagnosed multiple myeloma (MM) under strict regulations.[1] Thalidomides teratogenic properties led to the development of analogs with fewer side effects and increased potency which include lenalidomide which is marketed, pomalidomide which has an orphan drug status and apremilast which is in clinical phase, all of which are manufactured by Celgene.
History
[edit]The problems with thalidomide which led to the development of its analogs were, aside from the teratogenic side effects, both high incidence of other adverse reactions along with poor solubility in water and absorption from the intestines. [2] Adverse reactions include peripheral neuropathy in large majority of patients, constipation, thromboembolism along with dermatological complications. [3] Thalidomide came back to the market due to the discovery of its ability to inhibit tumour necrosis factor-alpha (TNF-α), previously unknown. TNF-α is a cytokine produced by macrophages of the immune system, and also the primary mediator of inflammatory response. Thus the drug is effective against numerous inflammatory diseases. Now with new indication thalidomide gained FDA approval for treatment of erythema nodosum leprosum (ENL), an immuno-inflammatory disease in patients with leprosy.[2] Four years after thalidomide was withdrawn from the market its inflammatory properties were discovered when patients suffering from ENL used thalidomide as a sedative and it reduced both the clinical signs and symptoms of the disease. This discovery increased the interest of further research and synthesis of safer analogs of thalidomide along with establishing thalidomides use in the treatment of chronic inflammatory diseases and as an immunomodulary drug. [4]
Lenalidomide is the first analog of thalidomide which is marketed. It is considerably more potent than its parent drug with only two differences at a molecular level, with an added amino group at position 4 of the phthaloyl ring and removal of a carbonyl group from the phthaloyl ring. [5] Development of lenalidomide began in the late 1990’s and clinical trials of lenalidomide began in 2000. In October 2001 lenalidomide was granted orphan status for the treatment of MM. In mid-2002 it entered phase II and by early 2003 phase III. In February 2003 FDA granted fast-track status to lenalidomide for the treatment of relapsed or refractory MM.[4] In 2006 it was approved for the treatment of MM along with dexamethasone and in 2007 by European Medicines Agency (EMA). In 2008, phase II trial observed efficacy in treating Non-Hodgkin's lymphoma.[6] Pomalidomide (3-aminothalidomide) was the second thalidomide analog to enter the clinic being more potent than both of its predecessors. [7] First documented in 1999 pomalidomide was noted to directly inhibit myeloma cell proliferation and thus inhibiting MM both on the tumor and vascular compartments.[8] In vitro studies measuring anti-TNF-α demonstrated that pomalidomide is 5000 times more potent than thalidomide and 10 times more potent than lenalidomide. In addition to being more potent is has fewer side effects than thalidomide.[9] First mention of apremilast dates back to 2005.[10]
Pharmacokinetics
[edit]Thalidomide
[edit]| Thalidomide | ||
|---|---|---|
| Tmax [drug] | 4 - 6 hours in subjects with MM. [11] |
|
| Protein binding | 55 – 65% [12] | |
| Metabolites | Hydrolized metabolites [12] | |
| Half-life [t1/2] | 5,5 – 7,6 hours [12] | |
Lenalidomide
[edit]| Lenalidomide | ||
|---|---|---|
| Tmax [drug] | 0,6 – 1,5 hours in healthy subjects [13] 0,5 – 4 hours in subjects with MM [14] |
.png)
|
| Protein binding | ~30% [13] | |
| Metabolites | Has not yet been studied [13] | |
| Half-life [t1/2] | 3 hours in healthy subjects [13] 3,1 – 4,2 hours in subjects with MM [14] | |
Pomalidomide
[edit]| Pomalidomide | ||
|---|---|---|
| Tmax [drug] | 0,5 – 8 hours [15] |
.png)
|
| Protein binding | Unknown | |
| Metabolites | Unknown | |
| Half-life [t1/2] | 6,2 – 7,9 hours [15] | |
Apremilast
[edit]| Apremilast | ||
|---|---|---|
| Tmax [drug] | 1,5 – 2 hours in healthy subjects.[16] Median 2 hours in patients with severe-plaque type psorasis. [17] |
|
| Protein binding | ~90% [18] | |
| Metabolites | O-desmethyl apremilast glucuronide and demethylated apremilast along with hydrolyzed products. [16] | |
| Half-life [t1/2] | 8,2 hours [17] | |
Mechanism of action
[edit]Thalidomide and its analogs, lenalidomide and pomalidomide, are believed to act in a similar fashion even though their exact mechanism of action is not yet fully understood. It is believed that they work through different mechanisms in various diseases. The net effect is probably due to different mechanisms combined. Apremilast is, however, believed to work through a different mechanism. Mechanism of action will be explained in light of today's knowledge, mainly in MM (Figure 2).
Thalidomide, lenalidomide and pomalidomide
[edit]
Altering cytokine production
[edit]Thalidomide and its immune-modulating analogs alter the production of the inflammatory cytokines TNF-α, IL-1, IL-6, IL-12 and anti-inflammatory cytokine IL-10.[19] The drugs are believed to inhibit the production of TNF-α, where the analogs are up to 50.000 times more potent in vitro than the parent drug thalidomide.[20] The mechanism is believed to be through enhanced degradation of TNF-α mRNA, resulting in diminished amounts of this pro-inflammatory cytokine secreted.[21] This explains the effect of thalidomide when given to ENL patients, as they commonly have high levels of TNF-α in their blood and in dermatological lesions. [4] In contrast, in vitro assay demonstrated that TNF-α is actually enhanced in T-cell activation, where CD4+ and CD8+ T lymphocytes were stimulated by anti-CD3 [20][4] which was later confirmed in an early phase trials involving solid tumors and inflammatory dermatologic diseases. [21] IL-12 is another cytokine both suppressed and enhanced by thalidomide and its analogs. When monocytes are stimulated by lipopolysaccharides, IL-12 production is suppressed but during T-cell stimulation the production is enhanced.[20]
Lenalidomide is believed to be about 1000 times more potent in vitro than thalidomide in anti-inflammatory properties and pomalidomide about 10 times more potent than lenalidomide. It is worth noticing however that, when comparing lenalidomide and pomalidomide, clinical relevance of higher in vitro potency is unclear since maximum tolerated dose of pomalidomide is 2 mg daily compared to 25 mg for lenalidomide, leading to 10-100 times lower plasma drug concentration of pomalidomide. [22]
T-cell activation
[edit]Thalidomide and its analogs help with the co-stimulation of T-cells through the B7-CD28 complex by phosphorylating tyrosine on the CD28 receptor.[4] In vitro data suggests this co-stimulation leads to increased Th1 type cytokine release of IFN-γ and IL-2 that further stimulates clonal T cell proliferation and natural killer cell proliferation and activity. This enhances natural and antibody dependent cellular cytotoxicity.[23]Lenalidomide and pomalidomide are about 100-1000 times more potent in stimulating T-cell clonal proliferation than thalidomide. In addition, in vitro data suggests pomalidomide reverts Th2 cells into Th1 by enhancing transcription factor T-bet.[19]
Anti-angiogenesis
[edit]Angiogenesis or the growth of new blood vessels has been reported to correspond with MM progression where vascular endothelial growth factor (VEGF) and its receptor, TNF-α [19], bFGF [4] and IL-6 [20] appear to be required for endothelial cell migration during angiogenesis. Thalidomide and its analogs are believed to suppress angiogenesis through modulation of the above mentioned factors where potency in anti-angiogenic activity for lenalidomide and pomalidomide was 2-3 times higher than for thalidomide in various in vivo assays,[24] This is in contrast to in vitro data which showed thalidomide to be about 10 times more potent than its analogs in anti-angiogenesis.[22] Thalidomide has also been shown to block NF-κB activity through the blocking of IL-6, but NF-κB has been shown to be essential for angiogenesis.[20]
Anti-tumor activity
[edit]In vitro assays on apoptosis in MM cells have been shown, when treated with thalidomide and its analogs, to up-regulate the activity of caspase8. This causes cross talking of apoptotic signaling between caspase8 and caspase9 leading to indirect up-regulation of caspase9 activity.[19][21] Further anti-tumor activity is mediated through the inhibition of apoptosis protein-2 [24] and pro-survival effects of IGF-1, increasing sensitivity to FAS mediated cell death and enhancement of TNF-related apoptosis inducing ligand.[21]They have also been shown to cause dose dependent G0/G1 cell cycle arrest in leukemia cell lines [20] where the analogs showed 100 times more potency than thalidomide.[22]
Bone marrow environment
[edit]The inflammatory responses within the bone marrow are believed to foster many hematological diseases. The secretion of IL-6 by bone marrow stromal cells (BMSC) and the secretion of the adhesion molecules VCAM-1, ICAM-1 and LFA, is induced in the presence of TNF-α and the adhesion of MM cells to BMSC . In vitro proliferation of MM cell lines and inhibition of Fas-mediated apoptosis is promoted by IL-6 .[21] Thalidomide and its analogs directly decrease the up-regulation of IL-6 and indirectly through TNF-α, thereby reducing the secretion of adhesion molecules leading to fewer MM cells adhering to BMSC. Osteoclasts become highly active during MM, leading to bone resorption and secretion of various MM survival factors. They decrease the levels of adhesion molecules paramount to osteoclast activation, decrease the formation of the cells that form osteoclasts and down-regulate cathepsin K, an important cysteine protease expressed in osteoclasts. [24]
Apremilast
[edit]In vitro, Apremilast reduces PDE4 activity leading to an increase in cyclic-adenosine monophosphate (cAMP) concentrations in immune and non-immune cell types, partially inhibiting the production of many pro-inflammatory cytokines such as TNF-α, IFN-γ IL-2, IL-12 and IL-23 and elevating the production of the anti-inflammatory cytokine IL-10. [25][26] The inhibition potency of apremilast in TNF-α production is similar to lenalidomide.[27]
Teratogenicity
[edit]Thalidomide‘s teratogenicity has been a subject to much debate and over the years numerous hypothesis have been proposed. Two of the most famous one's have been the anti-angiogenesis hypothesis and oxidative stress model hypothesis with considerable experimental evidence linking these two hypothesis to thalidomide's teratogenicity.[28]
Recently, new findings have emerged that suggest a novel mechanism of teratogenicity. Cereblon is a 51 kDa protein localized in the cytoplasm, nucleus and peripheral membrane of numerous parts of the body.[19] It acts as a component of the E3 ubiquitin ligase, regulating various developmental processes, including embryogenesis, carcinogenesis and cell cycle regulation, through degradation (ubiquitination) of unknown substrates. Thalidomide has been identified to bind to cereblon, inhibiting the activity of the E3 ubiquitin ligase, resulting in accumulation of the ligase substrates and downregulation of fibroblast growth factor 8 (FGF8) and FGF10. This disrupts the positive feedback loop between the two growth factors, possibly causing multiple birth defects and anti-myeloma effects. Findings also support that cereblon is an essential element for the anti-myeloma effect of both lenalidomide and pomalidomide.[28] Cereblon expression was three times higher in responding patients compared to non-responders and higher cereblon expression was also associated with partial or full response while lower expression was associated with stable or progressive disease.[19]
Indications
[edit]Thalidomide
[edit]Thalidomide has been approved by the FDA for ENL and MM in combination with dexamethasone. EMA has also approved it to treat MM in combination with prednisone and/or melphalan. Orphan indications by the FDA include graft versus host disease, mycobacterial infection, recurrent aphthous ulcers, severe recurrent aphthous stomatitis, primary brain malignancies, HIV-associated wasting syndrome, Crohn's disease, Kaposi’s sarcoma, myelodysplastic syndrome and hematopoietic stem cell transplantantion.[12][29]
Lenalidomide
[edit]Lenalidomide is approved in nearly 70 countries, in combination with dexamethasone for the treatment of patients with MM who have received at least one prior therapy. Orphan indications include diffuse large B-cell lymphoma, chronic lymphocytic leukemia and mantle cell lymphoma. Lenalidomide is also approved for transfusion-dependent anemia due to low or intermediate-1-risk myelodysplastic syndromes associated with a deletion 5q cytogenetic abnormality with or without additional cytogenetic abnormalities in the US, Canada, Switzerland, Australia, New Zealand, Malaysia, Israel and several Latin American countries, while marketing authorization application is currently being evaluated in a number of other countries. [30] [31] Numerous clinical trials are already in the pipeline or being conducted to explore further use for lenalidomide, alone or in combination with other drugs. Some of these indications include acute myeloid leukemia , follicular lymphoma, MALT lymphoma, Waldenström macroglobulinemia, lupus erythematosus, Hodgkin's lymphoma, myelodysplastic syndrome and more. [32][33]
Pomalidomide
[edit]Pomalidomide was submitted for FDA approval on 26th of April 2012 [34] and on 21st of June it was announced that the drug would get standard FDA review. A marketing authorization application was filed to EMA 21st of June 2012, where a decision could come as soon as early 2013. EMA has already granted pomalidomide an orphan designation for primary myelofibrosis, MM, systemic sclerosis, post-polycythaemia and post-essential thrombocythaemia myelofibrosis.[35]
Apremilast
[edit]As of September 2012 apremilast is in phase III trials for psoriasis, phase III for psoriatic arthritis and phase II trials for rheumatoid arthritis. Efficacy in ankylosing spondylitis is also being tested.[25]
Development
[edit]The thalidomide molecule is a synthetic derivative of glutamic acid and consists of a glutarimide ring and a phthaloyl ring [18] [36] Its IUPAC name is (2-(2,6-dioxo-3-piperidyl)isoindoline-1,3dione) and it has one chiral center [18] After thalidomide‘s selective inhibiton of TNF-α had been reported a renewed effort was put in thalidomide's clinical development. The clinical development led to the discovery of new analogs which strived to have improved activities and decreased side-effects. [4][37] Clinically thalidomide has always been used as a racemate. Generally the S-isomer is associated with the infamous teratogenic effects of thalidomide and the R- isomer is devoid of the teratogenic properties but conveys the sedative effects.[4] This view is highly debated and it has been argued that the animal model that these different R- and S- effects were seen in was not sensitive to the thalidomide teratogenic effects. Later reports in rabbits, which is a sensitive species, unveiled teratogenic effects from both isomers.[4][37][18][36] Moreover the thalidomide enantiomers have been shown to be interconversed in vivo, due to the acidic chiral hydrogen in the asymmetric center [36] [37] , so the plan to administer a purified single enantiomer to avoid the teratogenic effects will most likely be in vain.[4][18][36]
Development of lenalidomide and pomalidomide
[edit]One of the analogs of interest was made by isoindolinone replacement of the phthaloyl ring. It was given the name EM-12 (Figure 3). This replacement was thought to increase the bioavailability of the substance because of increased stability. The molecule had been reported to be an even more potent teratogenic agent than thalidomide in rats, rabbits and monkeys. In a study to further improve the TNF-α inhibitory activity of both thalidomide and the EM-12 analog, amino-phthaloyl substitutions were performed. The analogs that showed the most promise were the 4-amino analog of thalidomide, which had 15.000 times more potent TNF-α inhibitory activity than thalidomide in vitro, and the 4-amino substituted analog of EM-12 which was also a potent inhibitor of TNF-α.[36] These two analogs later got the name lenalidomide, which is the EM-12 amino analog, and pomalidomide, the thalidomide amino analog.[4]
Development of Apremilast
[edit]After finding a novel set of analogs of thalidomide, namely 3-(1,3-dioxo-1,3 dihydroisoindol-2-yl)-3-(3,4-dimethoxyphenyl) propionic acid (Not shown), which had PDE4 inhibition activity the work began to optimize the activity. For that purpose the researchers used a known structure moiety, 3,4-dialkoxyphenyl, that is a recognized pharmacophore in PDE4 inhibitors such as rolipram(figure 5) and roflumilast and added it onto the structure of the previously mentioned analog series. After tweaking the structure and testing different substitution at the 4-position of the phthaloyl ring and at the carboxyl acid the researchers finally landed at a molecule that potently inhibits PDE4 and TNF-α, which they later named apremilast. The S-enantiomer of apremilast was chosen since it was the more active enantiomer and was therefore chosen.[18] Since the structure of apremilast lacks the acidic chiral hydrogen it should not racemate in vivo, unlike thalidomide, lenalidomide and pomalidomide.[37][36]
Structure activity relationship
[edit]
Since the mechanism of action of thalidomide and its analogs is not fully clear and the bioreceptor for these substances has not been identified, the insight into the relationship between the structure and activity of thalidomide and its analogs are mostly derived from molecular modelling and continued research investigation.[38][37] The information on SAR of thalidomide and it‘s analogs is still in process so any trends detailed here are observed during individual studies. Research has mainly focused on improving the TNF-α and PDE4 inhibition of thalidomide [4][18], as well as the anti-angiogenesis activity. [39][40]
TNF-α inhibitors (not via PDE4)
[edit]Research indicated that a substitution at the phthaloyl ring would increase TNF-α inhibition activity (Figure 4). An amino group substitution was tested at various locations on the phthaloyl ring (C4,C5,C6,C7) of thalidomide and EM-12 (previously described). Amino addition at the C4 location on both thalidomide and EM-12 resulted in much more potent inhibition of TNF-α. This also revealed that the amino group needed to be directly opposite the carbonyl group on the isoindolinone ring system for the most potent activity.[41] These analogs do not inhibit PDE4 and therefore do not act by PDE4 inhibition. Other additions of longer and bigger groups at the C4 and C5 position of the phthaloyl ring system of thalidomide, some with an olefin functionality, have been tested with various results. Increased inhibitory effect, compared to thalidomide, was noticed with the groups that had an oxygen atom attached directly to the C5 or C4 olefin. Iodine and bromide addition at C4 or C5 resulted in equal or decreased activity compared to thalidomide.[42] These groups were not compared with lenalidomide or pomalidomide.
PDE4 inhibitors
[edit]
.png)
The common structure for analogs that inhibit TNF-α via inhibition of PDE4 is prepared on the basis of hydrolysing the glutarimide ring of thalidomide. These analogs do not have an acidic chiral hydrogen, unlike thalidomide, and would therefore be expected to be chirally stable. [36]
On the phenyl ring a 3,4 dialkoxyphenyl moiety (Figure 5) is a known pharmacophore in PDE4 inhibitors such as rolipram. Optimal activity is achieved with a methoxy group at the 4-position (X2) and a bigger group, such as cyclopentoxy at the 3-position carbon (X3). However the thalidomide PDE4 inhibitory analogs do not follow the SAR of rolipram analogs directly. For thalidomide analogs an ethoxy group at X3 and a methoxy group at X2, with X1 being just a hydrogen, gave the highest PDE4 and TNF-α inhibition.[18] Substitutes larger than diethoxy at the X2-X3 position had decreased activity. The effects of these substitution seem to be mediated by steric effects.[36]
For the Y-position a number of groups have been explored. Substituted amides that were larger than methylamide (CONHCH3) decreases PDE4 inhibition activity.[36] Using a carboxylic acid as a starting point, an amide group has similar PDE4 inhibition activity but both groups were shown to be a considerably less potent than a methyl ester group which had about 6 fold increase in PDE4 inhibitory activity. Sulfone group had similar PDE4 inhibition as the methyl ester group. The best PDE4 inhibtion was observed when a nitrile group was attached which was has 32 times more PDE4 inhibitory activity than the carboxyl acid.[18]
Substitution on the phthaloyl ring have been explored and it was noticed that nitro groups at the 4C or 5C location decreased activity but 4C or 5C amino substitution increased it dramatically.[36] When the substitution at the 4 (Z) location on the phthaloyl ring was examined, hydroxyl and methoxy groups seem to make the analog a less potent PDE4 inhibitor. An increase in activity was observed with amino and dimethylamino to a similar extent but a methyl group improved the activity further than the aforementioned groups. A 4-N-acetylamino group had slightly lower PDE4 inhibitory activity, compared with the methyl group, but increased the compounds TNF-α inhibitory activity to a further extent.[18]
Angiogenesis inhibition
[edit]
For angiogenesis inhibition activity, an intact glutarimide ring seems to be required. Different groups were tested in the R position. The substances that had nitrogen salts as the R group showed good activity. The improved angiogenesis inhibitory activity could be due to increased solubility or that the positively charged nitrogen has added interaction with the active site. Tetrafluorination of the phthaloyl ring seems to increase the angiogenesis inhibition.[39]
Synthesis
[edit]Described here are ways of synthesising thalidomide, lenalidomide, pomalidomide and apremilast.
Thalidomide
[edit]
Synthesis of thalidomide has usually been performed as seen in scheme 1. This synthesis is a reasonably simplistic three step process. The downside of this process however is that the last step requires a high-temperature melt reaction which demands multiple recrystallizations and is not compliant with standard equipment.
Scheme 2 is the newer synthesis route which was designed to make the reaction more direct and to produce better yields. This route uses L-glutamine rather than L-glutamic acid as a starting material and by letting it react with N-carbethoxyphthalimide gives N-phthaloyl-L-glutamine (4), with 50-70% yield. The substance 4 is then stirred in a mixture with carbonyldiimidazole (CDI) with enough 4-dimethylaminopyridine (DMAP) in tetrahydrofuran (THF) to catalyze the reaction and heated to reflux for 15-18 hours. During the reflux thalidomide crystallizes out of the mixture. The final step gives 85-93% yield of thalidomide. Bringing the total yield to 43-63%[43]
Lenalidomide and pomalidomide
[edit]
Both of the amino analogs are prepared from the condensation of 3-aminopiperidine-2,6-dione hydrochloride (Compound 3) which is synthesized in a two step reaction from commercially available Cbz-L-glutamine. The Cbz-L-glutamine is treated with CDI in refluxing THF to yield Cbz-aminoglutarimide. To remove the Cbz protecting group hydrogenolysis, under 50-60 psi of hydrogen with 10% Pd/C mixed with ethyl acetate and HCl, was performed. The formulated hydrochloride (Compound 3 in Scheme 3) was then reacted with 3-nitrophthalic anhydride in refluxing acetic acid to yield the 4-nitro substituted thalidomide analog and the nitro group then reduced with hydrogenation to give pomalidomide.[41]
Lenalidomide is synthesized in a similar way using compound 3 treated with a nitro-substituted methyl 2-(bromomethyl) benzoate, and hydrogenation of the nitro group.[41]
Apremilast
[edit]
In the synthesis of apremilast, compound 3 in scheme 5 is produced in a 41% yield from compound 2 treated with lithium hexamethyldisilazide, in mixture with lithium dimethylsulfone and boron trifluoride etherate. The resolution of compound 3 was accomplished by treating it with N-acetyl-L-leucine yielding 3S. The final step then uses condensation of 3-N-acetylaminophthalic anhydride with 3S to afford apremilast (1S) in 75% yield.[18]
See also
[edit]References
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{{cite journal}}: Check|s2cid=value (help)CS1 maint: multiple names: authors list (link) - ^ a b Mazzoccoli, L.; Cadoso, S. H.; Amarante, G. W.; De Souza, M. V.; Domingues, R.; Machado, M. A.; De Almeida, M. V.; Teixeira, H. C. (2012 Jul). "Novel thalidomide analogues from diamines inhibit pro-inflammatory cytokine production and CD80 expression while enhancing IL-10". Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie. 66 (5): 323–9. doi:10.1016/j.biopha.2012.05.001. PMID 22770990.
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