User:Andrew Su/ITK (gene)

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IL2-inducible T-cell kinase
1LUI​
Available structures: 1lui​, 1luk​, 1lum​, 1lun​, 1sm2​, 1snu​, 1snx​, 2etz​, 2eu0​
Identifiers
Symbols	ITK; PSCTK2; EMT; LYK; MGC126257; MGC126258
External IDs	OMIM: 186973 MGI: 96621 HomoloGene: 4051
EC number	2.7.10.2
Gene ontology Molecular function: • nucleotide binding • non-membrane spanning protein tyrosine kinase activity • ATP binding • zinc ion binding • kinase activity • transferase activity • metal ion binding Biological process: • protein amino acid phosphorylation • cellular defense response • intracellular signaling cascade
RNA expression pattern
More reference expression data
Protein domains
Orthologs

Category:Human proteins

IL2-inducible T-cell kinase, also known as ITK, is a protein

which in humans is encoded by the ITK  gene.

Function

This gene encodes an intracellular tyrosine kinase

expressed in T-cell

s. The protein is thought to play a role in T-cell proliferation and differentiation.

Structure

This protein contains the following domains, which are often found in intracellular kinases:

• N-terminus – PH

(pleckstrin homology domain)

• BTK – Bruton's tyrosine kinase Cys-rich motif
• SH3

– (Src homology 3)

• SH2

– (Src homology 2)

• C-terminus – tyrosine kinase, catalytic domain

Protein interactions

The protein encoded by this gene has been found to interact with FYN , GRB2 , LAT , KHDRBS1 , KPNA2 , LCP2 , PPIA , PLCG1 , and WAS

in multiple protein interaction studies.

Further reading

• Kosaka Y, Felices M, Berg LJ (2006). "Itk and Th2 responses: action but no reaction". Trends Immunol. 27 (10): 453–60. doi:10.1016/j.it.2006.08.006. PMID 16931156.{{cite journal}}: CS1 maint: multiple names: authors list (link)
• Langhans-Rajasekaran SA, Wan Y, Huang XY (1995). "Activation of Tsk and Btk tyrosine kinases by G protein beta gamma subunits". Proc. Natl. Acad. Sci. U.S.A. 92 (19): 8601–5. doi:10.1073/pnas.92.19.8601. PMID 7567982.{{cite journal}}: CS1 maint: multiple names: authors list (link)
• Janis EM, Siliciano JD, Isaac DD; et al. (1995). "Mapping of the gene for the tyrosine kinase Itk to a region of conserved synteny between mouse chromosome 11 and human chromosome 5q". Genomics. 23 (1): 269–71. doi:10.1006/geno.1994.1492. PMID 7829087. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: multiple names: authors list (link)
• Gibson S, Leung B, Squire JA; et al. (1993). "Identification, cloning, and characterization of a novel human T-cell-specific tyrosine kinase located at the hematopoietin complex on chromosome 5q". Blood. 82 (5): 1561–72. doi:10.1182/blood.V82.5.1561.1561. PMID 8364206. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: multiple names: authors list (link)
• Tanaka N, Asao H, Ohtani K; et al. (1993). "A novel human tyrosine kinase gene inducible in T cells by interleukin 2". FEBS Lett. 324 (1): 1–5. doi:10.1016/0014-5793(93)81520-A. PMID 8504851. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: multiple names: authors list (link)
• Rawlings DJ, Scharenberg AM, Park H; et al. (1996). "Activation of BTK by a phosphorylation mechanism initiated by SRC family kinases". Science. 271 (5250): 822–5. doi:10.1126/science.271.5250.822. PMID 8629002. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: multiple names: authors list (link)
• Park H, Wahl MI, Afar DE; et al. (1996). "Regulation of Btk function by a major autophosphorylation site within the SH3 domain". Immunity. 4 (5): 515–25. doi:10.1016/S1074-7613(00)80417-3. PMID 8630736. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: multiple names: authors list (link)
• Bunnell SC, Henry PA, Kolluri R; et al. (1996). "Identification of Itk/Tsk Src homology 3 domain ligands". J. Biol. Chem. 271 (41): 25646–56. doi:10.1074/jbc.271.41.25646. PMID 8810341. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: multiple names: authors list (link)
• Andreotti AH, Bunnell SC, Feng S; et al. (1997). "Regulatory intramolecular association in a tyrosine kinase of the Tec family". Nature. 385 (6611): 93–7. doi:10.1038/385093a0. PMID 8985255. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: multiple names: authors list (link)
• King PD, Sadra A, Teng JM; et al. (1997). "Analysis of CD28 cytoplasmic tail tyrosine residues as regulators and substrates for the protein tyrosine kinases, EMT and LCK". J. Immunol. 158 (2): 580–90. doi:10.4049/jimmunol.158.2.580. PMID 8992971. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: multiple names: authors list (link)
• Heyeck SD, Wilcox HM, Bunnell SC, Berg LJ (1997). "Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity". J. Biol. Chem. 272 (40): 25401–8. doi:10.1074/jbc.272.40.25401. PMID 9312162.{{cite journal}}: CS1 maint: multiple names: authors list (link)
• De Sepulveda P, Okkenhaug K, Rose JL; et al. (1999). "Socs1 binds to multiple signalling proteins and suppresses steel factor-dependent proliferation". EMBO J. 18 (4): 904–15. doi:10.1093/emboj/18.4.904. PMID 10022833. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: multiple names: authors list (link)
• Kaukonen J, Savolainen ER, Palotie A (1999). "Human Emt tyrosine kinase is specifically expressed both in mature T-lymphocytes and T-cell associated hematopoietic malignancies". Leuk. Lymphoma. 32 (5–6): 513–22. doi:10.3109/10428199909058409. PMID 10048424.{{cite journal}}: CS1 maint: multiple names: authors list (link)
• Shan X, Wange RL (1999). "Itk/Emt/Tsk activation in response to CD3 cross-linking in Jurkat T cells requires ZAP-70 and Lat and is independent of membrane recruitment". J. Biol. Chem. 274 (41): 29323–30. doi:10.1074/jbc.274.41.29323. PMID 10506192.
• Su YW, Zhang Y, Schweikert J; et al. (1999). "Interaction of SLP adaptors with the SH2 domain of Tec family kinases". Eur. J. Immunol. 29 (11): 3702–11. doi:10.1002/(SICI)1521-4141(199911)29:11<3702::AID-IMMU3702>3.0.CO;2-R. PMID 10556826. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: multiple names: authors list (link)
• Perez-Villar JJ, Kanner SB (2000). "Regulated association between the tyrosine kinase Emt/Itk/Tsk and phospholipase-C gamma 1 in human T lymphocytes". J. Immunol. 163 (12): 6435–41. doi:10.4049/jimmunol.163.12.6435. PMID 10586033.
• Rajagopal K, Sommers CL, Decker DC; et al. (2000). "RIBP, a novel Rlk/Txk- and itk-binding adaptor protein that regulates T cell activation". J. Exp. Med. 190 (11): 1657–68. doi:10.1084/jem.190.11.1657. PMID 10587356. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: multiple names: authors list (link)
• Bunnell SC, Diehn M, Yaffe MB; et al. (2000). "Biochemical interactions integrating Itk with the T cell receptor-initiated signaling cascade". J. Biol. Chem. 275 (3): 2219–30. doi:10.1074/jbc.275.3.2219. PMID 10636929. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: multiple names: authors list (link)
• Shan X, Czar MJ, Bunnell SC; et al. (2000). "Deficiency of PTEN in Jurkat T cells causes constitutive localization of Itk to the plasma membrane and hyperresponsiveness to CD3 stimulation". Mol. Cell. Biol. 20 (18): 6945–57. doi:10.1128/MCB.20.18.6945-6957.2000. PMID 10958690. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: multiple names: authors list (link)
• Hawkins J, Marcy A (2001). "Characterization of Itk tyrosine kinase: contribution of noncatalytic domains to enzymatic activity". Protein Expr. Purif. 22 (2): 211–9. doi:10.1006/prep.2001.1447. PMID 11437596.

PDB gallery

• 
  1lui: NMR Structures of Itk SH2 domain, Pro287cis isoform, ensemble of 20 low energy structures
• 
  1luk: NMR Structure of the Itk SH2 domain, Pro287cis, Energy minimized average structure
• 
  1lum: NMR Structure of the Itk SH2 domain, Pro287trans, 20 low energy structures
• 
  1lun: NMR Structure of the Itk SH2 domain, Pro287trans, energy minimized average structure
• 
  1sm2: Crystal structure of the phosphorylated Interleukin-2 tyrosine kinase catalytic domain
• 
  1snu: Crystal structure of the unphosphorylated interleukin-2 tyrosine kinase catalytic domain
• 
  1snx: Crystal structure of apo interleukin-2 tyrosine kinase catalytic domain
• 
  2etz: The NMR minimized average structure of the Itk SH2 domain bound to a phosphopeptide
• 
  2eu0: The NMR ensemble structure of the Itk SH2 domain bound to a phosphopeptide