Ure2 internal ribosome entry site (IRES)
In molecular biology, the Ure2 internal ribosome entry site (IRES) is an RNA element present in the 5' UTR of the mRNA of Ure2. It allows 5' cap- and eIF4E-independent translation of an N-terminally truncated form of Ure2. This truncated form lacks the prion-forming domain.[1] It is a 104 nucleotide region, smaller than most viral IRES elements, which forms a stem-loop structure.[2] EIF2A represses this IRES resulting in an inhibition of translation of the N-terminally truncated Ure2.[3][4]
See also
[edit]References
[edit]- ^ Komar, AA; Lesnik, T; Cullin, C; Merrick, WC; Trachsel, H; Altmann, M (Mar 3, 2003). "Internal initiation drives the synthesis of Ure2 protein lacking the prion domain and affects [URE3] propagation in yeast cells". The EMBO Journal. 22 (5): 1199–209. doi:10.1093/emboj/cdg103. PMC 150336. PMID 12606584.
- ^ Reineke, LC; Komar, AA; Caprara, MG; Merrick, WC (Jul 4, 2008). "A small stem loop element directs internal initiation of the URE2 internal ribosome entry site in Saccharomyces cerevisiae". The Journal of Biological Chemistry. 283 (27): 19011–25. doi:10.1074/jbc.M803109200. PMC 2441550. PMID 18460470.
- ^ Komar, AA; Gross, SR; Barth-Baus, D; Strachan, R; Hensold, JO; Goss Kinzy, T; Merrick, WC (Apr 22, 2005). "Novel characteristics of the biological properties of the yeast Saccharomyces cerevisiae eukaryotic initiation factor 2A" (PDF). The Journal of Biological Chemistry. 280 (16): 15601–11. doi:10.1074/jbc.M413728200. PMID 15718232.
- ^ Reineke, LC; Cao, Y; Baus, D; Hossain, NM; Merrick, WC (2011). "Insights into the role of yeast eIF2A in IRES-mediated translation". PLOS ONE. 6 (9): e24492. Bibcode:2011PLoSO...624492R. doi:10.1371/journal.pone.0024492. PMC 3168509. PMID 21915340.
Further reading
[edit]- Reineke, LC; Merrick, WC (December 2009). "Characterization of the functional role of nucleotides within the URE2 IRES element and the requirements for eIF2A-mediated repression". RNA. 15 (12): 2264–77. doi:10.1261/rna.1722809. PMC 2779687. PMID 19861427.