Jump to content

Type I site-specific deoxyribonuclease

From Wikipedia, the free encyclopedia
Type I site-specific deoxyribonuclease
Identifiers
EC no.3.1.21.3
CAS no.37263-09-5
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Search
PMCarticles
PubMedarticles
NCBIproteins

Type I site-specific deoxyribonuclease (EC 3.1.21.3, type I restriction enzyme, deoxyribonuclease (ATP- and S-adenosyl-L-methionine-dependent), restriction-modification system, deoxyribonuclease (adenosine triphosphate-hydrolyzing), adenosine triphosphate-dependent deoxyribonuclease, ATP-dependent DNase, type 1 site-specific deoxyribonuclease) is an enzyme.[1] This enzyme catalyses the following chemical reaction

Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolysed

They have an absolute requirement for ATP (or dATP) and S-adenosyl-L-methionine.

See also

[edit]

References

[edit]
  1. ^ Roberts RJ (April 1990). "Restriction enzymes and their isoschizomers". Nucleic Acids Research. 18 Suppl: 2331–65. doi:10.1093/nar/18.suppl.2331. PMC 331877. PMID 2159140.
[edit]