Wikipedia:Featured picture candidates/Actin Filaments in a Cultured Cell

Actin Filaments in a Cultured Cell

Voting period is over. Please don't add any new votes. Voting period ends on 11 Jun 2015 at 00:25:04 (UTC)

Original – A z-projection of an osteosarcoma cell that was stained with phalloidin in order to visualise actin. Image was deconvoluted using an experimental point spread function and colour coded in the z-axis to better show the 3D position of the filaments.

Reason

Very high technical standard, high resolution image, gives insight into the complexity of cells (this is the network formed by just one of the cell's cytoskeletal proteins) aesthetically pleasing, has scale and metadata.

Articles in which this image appears

Deconvolution, Confocal microscope, Fluorescence microscope

FP category for this image

Wikipedia:Featured pictures/Sciences/Biology

Creator

methylated603

• Support as nominator – Methylated603 (talk) 00:25, 1 June 2015 (UTC)
• Question I used to make pictures like these! Yours is much better than my best. I'm just wondering about the colours. Presumably you used a single fluorescent protein, so what exactly are the colours showing? Are the red higher in the z-stack and purple lower? Mattximus (talk) 02:37, 1 June 2015 (UTC)

• @Mattximus: Thanks, yeah I used phalloidin (it would have conjugated to a 488 fluorophore) and took images every 80nm from the bottom to the top to get what the filament distribution is like in the whole cell. To show it in 2D I've depth coded it then merged it so each slice in 'z' is a slightly different colour allowing you to see where abouts the filaments are with regards to the cell height. If you look closely at the full resolution image you'll see that there is posterization in some places, this is intentionally left as thats where one slice ends and the next begins. I took this on an inverted microscope, the orange/red is at the cell base (what was attached to the coverslip) and the purple is at the very top of the cell. Methylated603 (talk) 05:20, 1 June 2015 (UTC)

• Support - I'll just pretend I understood a word of the above. Tra la la, pretty! — Chris Woodrich (talk) 07:12, 1 June 2015 (UTC)

What's the measurement – mm.? Sca (talk) 15:27, 1 June 2015 (UTC)

Micrometre Mattximus (talk) 23:18, 1 June 2015 (UTC)

• Support -Jobas (talk) 01:00, 5 June 2015 (UTC)
• Support Impressive. -- Slaunger (talk) 07:12, 5 June 2015 (UTC)
• Support But please add it to the "Nightmarish chicken monster" article (I'm definitely not eating cheese before I go to bed tonight or that will be chasing me through my dreams) Belle (talk) 14:28, 5 June 2015 (UTC)

Promoted File:Depth Coded Phalloidin Stained Actin Filaments Cancer Cell.png --Armbrust ^{The Homunculus} 02:52, 11 June 2015 (UTC)