User talk:Winter20jb/sandbox

Review comments: I think you made some good changes to the article, although you did not add very much content. Your images were good. You added information about how to break the patch, the advantage of cell-attached patches, and about the diameter of a pipette tip.

As far as I can tell, these are the changes you have made:

ADDED: "to obtain a gigaseal...By only attaching to the exterior of the cell membrane, there is very little disturbance to the structure and environment of the cell....or sometimes multiple...any intracellular activity normally influenced by the channel will still be able to function as it would physiologically. Using this method it is also relatively easy to obtain the right configuration and once obtained it is fairly stable"
minor edits made to sections
ADDED: "and another electrode in the bath around the cell. The diameter of the pipette tip can change depending on what is trying to be measured, but it is usually in the micrometer range,"
ADDED: "Once the pipette is attached to the cell membrane, there are two methods of breaking the patch. The first is by applying more suction. The amount and duration of this suction depends on the type of cell and size of the pipette. The other method requires a large current pulse to be sent through the pipette. Again how much current and for how long depend on the situation"

Suggestions:
1. Fix this sentence - spelling error and make it less of a fragment:

"Althought it is usually not possible to then change the drug concentration."

2. You wrote: "The diameter of the pipette tip can change depending on..."
My suggestion: this sounds like the diameter of each pipette tip is variable and adjustable. I recommend changing it to "can be changed" or something like that.
3. The way this section was changed makes it less understandable for those seeking to learn about patch clamps.
Your version:
Whole-cell recordings involve recording currents through multiple channels simultaneously, over the membrane of the entire cell. Once the pipette is attached to the cell membrane, there are two methods of breaking the patch. The first is by applying more suction. The amount and duration of this suction depends on the type of cell and size of the pipette. The other method requires a large current pulse to be sent through the pipette. Again how much current and for how long depend on the situation.[5]This provides access to the intracellular space of the cell.
Old version:
Whole-cell recordings, in contrast, involve recording currents through multiple channels at once, over the membrane of the entire cell. The electrode is left in place on the cell, but more suction is applied to rupture the membrane patch, thus providing access to the intracellular space of the cell.
In the original version, they gave the reason why they were breaking the patch right at the beginning, but you have inserted some information about how to break the patch before this. I recommend placing your new information AFTER the reason why the patch is being broken, otherwise this section becomes too confusing.
4. Fix this spelling error: "There is also a large input resistance which allows for clearer measurments"
5. Old Version: "A disadvantage of this technique is that the volume..."
Your Version: "A factor that may be a disadvantage to this technique is that because the volume..."
Your version makes it a lot more wordy. I recommend staying with the original wording.
6. You changed: "Thus, any properties of the cell..."
To: "After a while, any properties of the cell..."
This change makes it sound like it is a slower, gradual process. Is it a slower process? If not, consider changing it back.
7. You added: "Again how much current and for how long depend on the situation" This sentence seems kindof awkward, and like unnecessary detail.I recommend taking it out or re-wording it. 8. In the first paragraph of "Cell-attached patch" you basically say the same thing twice, about how the interior is not disturbed, but separated by another sentence. I recommend either removing of the extra sentences or putting them together.
9. You wrote: "depending on what is trying to be measured" which sounds like the cell is actively trying to be measured by the researcher. I recommend rewording this.

My responses to the review questions:

Is the web page suitable for first-time/general users as well as for those looking to understand the topic in more detail?
1. Yes, although I do have some suggestions (as listed above)
Is there a logical flow to the page?
1. Yes
Do the contents of each section justify its length?
1. Yes
Has a particular section been over-emphasized or under-emphasized compared to others?
1. No
Does the sandbox satisfy the aims/objectives listed in their outline?
Are all the important terms linked to their respective Wikipedia pages for further reference?
1. I don't think you added any links to other articles, but there weren't really any places to add them. You could try linking somewhere to explain what different cell types you are referring to in this statement: "The amount and duration of this suction depends on the type of cell and size of the pipette."
Do the images add to the educational value of the article?
1. yes
Are the references relevant and integrated well into the article?
1. yes
Rate the overall presentation of the webpage. Check for typos, hard-to-read images and equations or syntax errors.
1. see suggestions made above
Does the website satisfy all the assigned criteria (a minimum of one section, one figure, and three references per team member)?
1. yes

OLD COMMENTS I am trying to review your article, but it appears you have made almost no changes, or have not started working on it yet. The changes you made:
1. Removed two pictures
2. Added two references to existing content on the page

Neither of these are related to the assignment.

The assignment is:
1. Select one section (just one section, not an entire article) to improve.
2. Study three primary sources, and collect information from them about the section you are editing. This will take a lot of time, because you need to read three papers and study them until you understand how they can help you add to your wiki page.
3. Integrate this information into the section. You should add to/change the content significantly.
4. Add an image to your section. Make sure it is not copyrighted. You can create your own image if you wish. This image should help clarify the section's content for readers.

To give you an idea how much time this should take, my section took about 10-12 hours of work. I assume you are either working on your article somewhere else, or you haven't started editing it yet. When you do begin working on it, please make it easier to edit by only having the section you are working on in your sandbox instead of the whole article.

I hope the information I provided was useful. Stan.Mikita (talk) 23:49, 10 November 2014 (UTC)[reply]