User talk:Smithtyl/sandbox

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Tlefevre (talk) 13:13, 10 October 2017 (UTC)

Chi-Wen's Peer Review [CHEM 455]

Overall, I think you did a good job on the new version of this page.

One thing that I would recommend is in the section of application.

1. To make this paragraph more organized, I would recommend adding subtopics and talk different applications separately (experimental methods and clinical treatments).

2. This paragraph has less information, I would recommend finding more review papers or reports regarding the techniques (introducing in vivo and in vitro Protein-DNA crosslinking) and clinical treatments (reference any news regarding the clinical applications).

Sam Horrocks Chem 455 Peer Review

Like Chin Wen, I agree that overall you've done a very good job adding to this page.

I thought the breakdown of each exogenous and endogenous agent was done especially well.

Also though, I have a few suggestions in the Applications section:

1. What exactly is photocrosslinking? You mentioned it in the first paragraph, but never explained it? 2. Also, there are a few grammar things that would need to be touched up. For example, in the second sentence of the second paragraph, you say, "Cancer treatment have been..." but it should be "Cancer treatments have been..."

Mohamad Bazzi Peer Review

In the introduction section, I believe the contents are accessible to non-experts. The author introduces what they will be talking about, in this case, a topic in genetics, and hyperlinks important points with pages that will help the readers understand the topic better. Throughout the paper, the highlighted examples are useful because they provide more discussions about the exogenous agents they are talking about. However, I would appreciate if some of the important topics were explained throughout the paper, such as what is exogenous linking and the impact it can have on DNA mutations or diseases.

In the paper, the author the author talks about all various exogenous agents and their impact with DNA damage and repair; however, there aren’t any figures or resources that can help solidify the information the author talked about the agents. The information, in my opinion, they are very detailed and easily understood, but I would appreciate a figure or diagram to help solidify important points. For instance, in the following excerpt, the author mentions “When cisplatin generates DNA crosslinks, it more frequently forms 1,2-intrastrand crosslinks (5'-GG), but also forms 1,3-intrastrand crosslinks (5-GNG) at lower percentages.” For someone with limited scientific knowledge, I would like to see diagrams and detailed explanation in the diagrams about what crosslinks are, the types of crosslinks, and the method how cisplatin generates the DNA crosslinks.

Through the article, the author talks about the Repair of the DNA crosslinks, and I believe the descriptions and references are very descriptive and organized. It talks about the how the damage occurs, what is done to repair the crosslinks (homologous recombination pathway). This section of the Wikipedia page is well thought out, as well as they make the topic relevant to discussing it in human diseases; however, I would like to see more diagrams and figures with the repair, especially with homologous recombination pathway. I think the conclusion ties the paper together by giving the topic relevance to future studies and disease treatments.

Mabazzi8 (talk) 19:13, 24 October 2017 (UTC)

Essam Al-Snayyan Crosslinking of DNA [Peer Review]

The new version of this article does a very good job of introducing crosslink of DNA and discussing its multiple aspects. The information within the articles comes from numerous scientific articles. As a result, the language used is not directed toward a general audience can be improved upon. Additionally, the organization of certain sections of the article need work and certain topics, such as photocrosslinking, are introduced without real discussion.

In regards to organization, the following statement should be moved to the applications section of the article: “In humans, the leading cause of cancer deaths worldwide is lung cancer, including non small cell lung carcinoma (NSLC) which accounts for 85% of all lung cancer cases in the United States.[21] Individuals with NSLC are often treated with therapeutic platinum compounds (e.g. cisplatin, carboplatin or oxaliplatin) (see Lung cancer chemotherapy) that cause inter-strand DNA crosslinks” The reason why this should be move to applications is because it provides a good background on what crosslinking DNA might used for as a treatment.

Overall, the article does a good job of surveying crosslinking of DNA. I would suggest to work on the organization of the article, simplify the language used, and use more recent publications on the topic especially in the applications section.

Esnayyan1996 (talk) 19:38, 24 October 2017 (UTC)

Nils' Comments

This is a great start. Keep going, especially making the article adopt more laymen terms, figures and explanations (as the technical comment suggests); this should start in the first paragraph, trying not to lose the audience throughout.

MLibrarian's Topic Review

Great job on expanding the topic! And it is good you are planning on inserting some figures. Hope, it will work well now. In general, try explaining things as if to non-experts.

1. I would additionally try hyperlinking as many concepts as possible, e.g. molecule, DNA, chemicals, compounds
2. "These compounds intercalate into DNA at 5'-AT sequence sites and form thymidine adducts when activated in the presence of UV - A" - I would recommend spelling out UV-A
3. "H2O2 reactions" please use Wiki guide on chemical formulas https://en.wikipedia.org/wiki/Wikipedia:Manual_of_Style/Chemistry#Structure_drawing. We discussed this already but this guide also contains information on how to upload ChemDraw structure
4. "Summary Table of Crosslinking Agents" - please fill it in or remove unused columns MLibrarian (talk) 19:46, 30 October 2017 (UTC)

Gillian's Comments

Great job! I would try to include some figures that connect with your text. You could show the cross-linking of DNA or perhaps a figure with a cross-linking repair mechanism.