User talk:Mlee17/sandbox

Peer review by Renhe

Grammar and language

Nitrogenase is an enzyme responsible for catalyzing nitrogen fixation: the reduction of N2 to NH3 and a process vital to sustaining life on Earth.

• Better to use nitrogen and ammonium instead of N2 and NH3 for the first time.

Component I is a MoFe protein in molybdenum nitrogenase, a VFe protein in vanadium nitrogenase, and a Fe protein in iron-only nitrogenase.

• It would be better to say

Component II is aFe protein that contains Fe-S cluster

• Should have a space between a and Fe.

the the P-cluster (Figure 4), and the FeMo-cofactor (Figure 5).

• Should only have one "the"

Linkage and references

• It would be a good idea to link the other mentioned concepts to their wikipedia pages, like "diazotrophs" and "Iron–sulfur cluster".
• You don't have to cite one source for several times in one paragraph. You can cite it once at the end of this paragraph.

Figures and Structure

• It would be better to mark all the figures at the bottom of each picture/structure.
• Besides 3-D structure, it would be better to show the specific atoms like Fe and S in those clusters.

Other Topic

• For the FeMo-Co, can you show what does the spin mean in detail?
• It would be better to show some structure of isolated intermediate in the model complex to further support two N2 fixation pathways.
• If it is possible, can you show some recent application of nitrogenase (or some new discovery and mechanism study) ?

Summary

This is a very good article with clear structure. It covers all the important mechanism and also gives reliable citation.

--Rhli (talk) 05:14, 4 March 2017 (UTC)

Peer review by Chang Liu The new wiki page seems much more organized with sufficient information. I really like the parts of mechanism and the figures you have attache. And I only have several minor suggestions: For the first part, some figures seem a little repetitive, and the pattern may also need rearrangement. I would also suggest write about the aton label in the notes of each figures. For "intermediate from E0 to E4", I just wonder whether there is any structural change for these intermediates with the reaction goes. If is, are there any papers with relevant results. Anyway, the potential wiki page in your sandbox is really great! Lcaudrey (talk) 05:43, 4 March 2017 (UTC)

Peer Review by Vlad

Overall very good job, with a lot of details and sources. I would zoom in on both the pymol images and the cluster images. Actually, can the "cluster" figures be excluded from the article? You can lable them accordingly on the "active site" figure so the reader can easily zoom in and see what's going on. I would also suggest to delete either “Nitrogenase with active sites highlighted” or “Nitrogenase with Active Site Magnified”. The first sentence should be edited. Perhaps: “Nitrogenase is an enzyme responsible for catalyzing nitrogen fixation, which is the reduction of nitrogen (N2) to ammonia (NH3) and a process vital to sustaining life on Earth”. You link to nitrogen fixation more than once throughout the article, but it should only be done once (in your very first sentence). You refer to some figures, but they are not numbered in your article. I would also suggest using either "FeMo-co" or "FeMoco", but it must be consistent.

Vlisnyak (talk) 04:56, 5 March 2017 (UTC)