User talk:GomezChristian/sandbox

The mechanism has been expanded on quite well considering the exact mechanism has been the subject of intense debate. I like the "Some questions that remain..." statement, it is a good way to end the article. I would make the minor change of changing the first image caption to "Crystal Structure of Chorismate Mutase with a Bound Transition State Analog" AtreyiB (talk) 00:27, 3 March 2017 (UTC)atreyib[reply]

Agreed that this gives the study of chorismate mutase a good treatment, while signaling the open questions remaining. One edit: the list of active site residues at the end of "Protein Family" would probably be better placed somewhere in mechanism with a little explanation of what those residues are doing. Beyond that, maybe clarifying or simplifying some of the language in the "Protein Family" section --- for example: explain that e coli's CM is an archetypal example of the AroQ family which are characterized by a homodimeric three-helix bundle --- instead of chopping explanation up into fragments. Overall: solid addition to a far-too-brief article on a magnificent enzyme. You three are stars. Jmont31 (talk) 01:18, 3 March 2017 (UTC)[reply]

I think that the mechanism is defined well but I think that breaking up the mechanism into parts (like perhaps a subheading on the NAC) would make it more readable. I also think that a figure showing residues from the active site would really add to the article. http://www.pnas.org/content/94/16/8491/F1.expansion.html This was what I came up with from a quick search. If you guys wanted to look into adding something like this, I think it would be much more helpful than simply the reaction alone. I agree that your language could be simplified in parts. Overall the additions are good and most of the edits I see would be in favor of editing for clarity and accessibility. — Preceding unsigned comment added by Kitaylor (talk • contribs) 06:43, 3 March 2017 (UTC)[reply]

Jared notes====

1. should include a pymol image rather than only chemdraw 2. "The presence of chorismate mutase, increases" remove comma 3. "Additional studies have been done on the near attack conformer (NAC) of the reaction catalyzed by chorismate mutase." I think this needs to be written so that it emphasizes how studies were conducted to support the importance a putative NAC on the chorismate mutase reaction. the remainder of this paragraph is fine. 4. "Overall, there have been extensive studies on the exact mechanism of this reaction, but the rate-determining step has yet to be uncovered." this is not true. we know the RDS; there is just argument over why, exactly, the enzyme enhances rate. — Preceding unsigned comment added by Jared Lewis (talk • contribs) 19:25, 13 March 2017 (UTC)[reply]

The PyMOL image that is in the summary table is ours. The original one was a chemdraw figure showing the enzymatic reaction. GomezChristian (talk) 19:35, 13 March 2017 (UTC)[reply]

Altered last paragraph to account for comment 4. Charles Cole (talk) 19:58, 13 March 2017 (UTC)[reply]