User talk:AndrewHires

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Oh, and as you will agree, great minds know that they know nothing, that reality is relative, and that we aren’t very much without those that we trust in. But natural selection is rewarding those who grab power, like a law of nature. In the whole Universe. No exceptions. Which means that power corrupts. Everyone. So please don’t become a Wikinazi with a god complex. ^^ Seriously! :) — 88.77.189.202 (talk) 23:46, 15 September 2010 (UTC)[reply]

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In addition to the welcome, I like to thank you for outstanding work on the GFP article - much appreciated. -- MarcoTolo 22:38, 13 February 2007 (UTC)[reply]

Thanks! There is a lot more to do... Coral protein derivatives, genetically encoded sensors... This is getting addictive! AndrewHires 21:50, 15 February 2007 (UTC)[reply]
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Andrew, wanted to thank you for your "afterburn" and 2008 financial figures URL. Here's the link http://en.wikipedia.org/wiki/Talk:Burning_Man#Why_no_controversies.3F thanks again!! Signed, (Peter/SanDiego) Vid2vid (talk) 07:01, 1 October 2009 (UTC)[reply]
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More of those bacterial colony drawings?

Hey, do you have more of those pictures like the one below?

Because that would be very impressive, an should go to epicwinftw.com!
How about it? :)
— 88.77.189.202 (talk) 23:46, 15 September 2010 (UTC)[reply]

Wikipedia:Citing sources

Could you put in a source or two for that new paragraph at GFP? If you would prefer not to mess with the formatting, just reply here with enough information to track it down and I can take care of it. Regards, - Eldereft (cont.) 18:35, 13 November 2008 (UTC)[reply]

All that info was from a private lecture by Tsien. I couldn't find some of the data he showed in published papers, likely some figures were never included in publications. Here's an attempt at some proper cites.

The serine 65 residue of the GFP chromophore is responsible for the dual peaked excitation spectra of wild type GFP. It is conserved in all three GFP isoforms originally cloned by Prasher. ^ Prasher D, Eckenrode V, Ward W, Prendergast F, Cormier M (1992). "Primary structure of the Aequorea victoria green-fluorescent protein". Gene 111 (2): 229–33. doi:10.1016/0378-1119(92)90691-H. PMID 1347277.

Nearly all mutations of this residue consolidate the excitation spectra to a single peak at either 395nm or 480nm. Since a single mutation can make dramatically enhance the 480nm excitation peak,

Heim R, Cubitt AB, Tsien RY. 1995. Nature 373:663–64 Delagrave S, Hawtin RE, Silva CM, Yang MM, Youvan DC. 1995. Bio- Technology 13:151–14 Cormack BP, Valdivia RH, Falkow S.1996. Gene 173:33–38

Further discussion in ^ a b Tsien R (1998). "The green fluorescent protein" (PDF). Annu Rev Biochem 67: 509–44. doi:10.1146/annurev.biochem.67.1.509. PMID 9759496, http://tsienlab.ucsd.edu/Publications/Tsien%201998%20Annu.%20Rev.%20Biochem%20-%20GFP.pdf.

The speculations are from a lecture at Janelia Farm. Here is a video of his nobel lecture where he touches on similar points.

http://nobelprize.org/mediaplayer/index.php?id=1070

The population collapse at Friday Harbor is mentioned in this article by Miyawaki in Cell doi:10.1016/j.cell.2008.11.025

AndrewHires (talk) 04:05, 1 December 2008 (UTC)[reply]