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The blue highlighted line shows the location of the c10orf35 gene on Chromosome 10.

Chromosome 10 open reading frame 35 (c10orf35) is a gene that in humans, encodes for a protein-binding^[1], transmembrane^[2] protein. The protein contains the domain of unknown function 4605 (DUF4605)^[1] which belongs to the protein family pfam15378^[3]. This gene is located at locus 10q22.1^[1].

General Characteristics

The physical properties of the c10orf35 protein were analyzed, and the molecular weight was predicted to be 13.2 kdal^[4]. The isoelectric point was also predicted to be 11.5^[4]. The properties of DUF4605 were also analyzed, and the molecular weight was predicted to be 6.0 kdal^[4]. The isoelectric point was predicted to be 6.9^[4], which is significantly less basic than the full protein.

Protein Properties and Structure

This image gives a predicted image of the main secondary structure features, conserved regions, and the post-translational modification site.

This protein includes a higly conserved region between amino acids 1 and 19 discovered by MSA^[5]. DUF 4605 is located between amino acids 62 and 92 ^[1]. The secondary structure of the c10orf35 protein was predicted to consist of a β-sheet between amino acids 3 and 5^[6], an α-helix between 61 and 72^[6], and a transmembrane α-helix between 92 and 112^[7]. The serine in position 62 can be phosphorylated to form a phosphoserine group^[7].

Homology

Paralogs

The protein of interest has a similar, paralogous domain found in c4orf32^[8], which is conserved in mammals, birds, very few reptiles, and one amphibian^[9].

Orthologs

The protein coded for by c10orf35 has many orthologs found mainly in mammals, reptiles, few amphibians, one fish, and one invertebrate^[10]. Based on a multiple sequence alignment between representative orthologs, DUF 4605 and a second, highly conserved region was determined^[5].

Clinical Significance

Expression

The c10orf35 protein has expression over the 75th percentile in the brain, spinal chord, and male reproductive organs^[12]. Within the male reproductive system, the RNA is found within the testis and prostate, while the protein is expressed in the epididymis^[13]. In the brain, the RNA is found in the cerebral cortex, while the protein is expressed ubiquitously throughout the brain^[13].

Transcription Regulation

Protein Interactions

The c10orf35 protein has been experimentally shown to interact with 7 other proteins^[1] as shown below.

Interacting Protein Symbol	Definition
ATP1B3	ATPase, Na⁺/K⁺ Transporting, Beta 3 Polypeptide
CD3E	CD3e molecule, epsilon (CD3-TCR complex)
GABRE	Gamma-aminobutyric acid (GABA) A receptor, epsilon
HTR3C	5-hydroxytryptamine (serotonin) receptor 3C, ionotropic
LPAR1	Lysophosphatidic acid receptor 1
SGCD	Sarcoglycan, delta (35kDa dystrophin-associated glycoprotein)
ZACN	Zinc activated ligand-gated ion channel

References

^ ^a ^b ^c ^d ^e "c10orf35". National Center For Biotechnology Information (NCBI) Gene.
^ "SOSUI". Classification and Secondary Structure Prediction of Membrane Proteins. Retrieved 6 May 2016.
^ "Conserved Protein Domain Family DUF4605". National Center For Biotechnology Information (NCBI) Structure. Retrieved 6 May 2016.
^ ^a ^b ^c ^d "Compute pI/Mw". ExPASy Bioinformatics Research Portal. Retrieved 6 May 2016.
^ ^a ^b "CLUSTALW Alignment Tool". San Diego Supercomputer Ceter (SDSC). Retrieved 6 May 2016.
^ ^a ^b "A Protein Secondary Structure Prediction Server". JPred. Retrieved 9 May 2016.
^ ^a ^b "c10orf35". UniProt. Retrieved 9 May 2016.
^ "c4orf32". National Center For Biotechnology Information (NCBI) Gene. Retrieved 6 May 2016.
^ "Standard Nucleotide BLASTN queried for c4orf32". National Center For Biotechnology Information (NCBI) Basic Local Alignment Searth Tool (BLAST). Retrieved 6 May 2016.
^ "Standard Nucleotide BLAST queried for c10orf35". National Center For Biotechnology Information (NCBI) Basic Local Alignment Search Tool (BLAST). Retrieved 6 May 2016.
^ "Gene Expression Omnibus (GEO) Profile For GDS3834". National Center For Biotechnology Information (NCBI). Retrieved 9 May 2016.
^ "Gene Expression Omnibus (GEO) Profile". National Center For Biotechnology Information. Retrieved 6 May 2016.
^ ^a ^b "c10orf35". The Human Protein Atlas. Retrieved 6 May 2016.