User:Yohhans
BRTP 822
[edit]Lu
[edit]- Mechanism of Transcription
- Transcription
- Structure of RNA
- RNA Synthesis
- Three stages in transcription
- RNA polymerase
- E. coli RNA polymerase
- Sigma factors
- Lac operon
- Control of elongation in E. coli
- Eukaryotic RNA polymerases
- Three Eukaryotic RNA polymerases are multi-protein complexes that share many common subunits
- Promoter
- TATA box
- Initiator sequences (INR)
- CpG island promoter
- General transcription factors
- Transcription initiation
- Regulation of transcription elongation
Trujillo
[edit]- Part 1
- Part 2
- Part 3
- Part 4
Bakhat
[edit]Misc. intro stuff
- Central problem is to figure out how genes are selected for expression during growth, differentiation, or in response to the environment
- Need to determine how a gene is specified by cis-DNA
- cis-elements are promoters, enhancers, silencers and insulators
Gene regulation
[edit]First we are going to look at core promoter elements. The core promoter is a region that is upstream of the transcription start site (TSS +1), and it contains several elements that facilitate the binding of regulatory elements. These elements form the pre-initiation complex (PIC).
Core promoters are divided into two different classes: TSS is focused over a narrow region, or it is dispersed over a broad region (50-100bp). Some core promoter elements include BRE (GC rich region that is the TFIIB recognition site. CpG dinucleotide is very important here: [GC][GC][GA]CGCC, the TATA box (TATA[AT]A[AT][AG]), and the Inr. A promoter element downstream of the TSS is called a downstream core promoter element.
Despite the TATA box being the
Mott
[edit]Godfrey
[edit]Caplan
[edit]Gould
[edit]Wang
[edit]Old
[edit]
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