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The Ski complex is a multi-protein complex involved in the 3' end degradation of messenger RNAs in yeast.[1] The complex consists of three main proteins, the RNA helicase Ski2 and the proteins Ski3 and Ski8. This tetramer contains a 370 kDa core complex, containing N-terminal arms and C-terminal arms from Ski3. The helicase core of Ski2 is positioned by both the C-terminal of Ski3 and two subunits of Ski8. Helicase activities are initiated by the N-terminal arm and the Ski2 insertion domain.Cite error: A <ref> tag is missing the closing </ref> (see the help page). In yeast, the complex guides RNA molecules to the exosome complex for degradation via a fourth protein, called Ski7, which contains a GTPase-like protein.[2] Ski7 involves the 3’ to 5’ degradation of RNA through two different pathways, 3’ poly(A) tail shortening and the binding of the Ski2, Ski3, and Ski8 tetramer and the exosome.[3] The complex consists of three main proteins, the RNA helicase Ski2 and the proteins Ski3 and Ski8. This tetramer contains a 370 kDa core complex, containing N-terminal arms and C-terminal arms from Ski3. The helicase core of Ski2 is positioned by both the C-terminal of Ski3 and two subunits of Ski8. Helicase activities are initiated by the N-terminal arm and the Ski2 insertion domain.[4] Biochemical studies also show that the Ski complex can thread RNA through the exosome complex, thereby coupling the Ski2 protein helicase function with the exoribonuclease activity, leading to degradation of the RNA strand. [5]
See also
[edit]References
[edit]- ^ Araki; Takahashi, S; Kobayashi, T; Kajiho, H; Hoshino, S; Katada, T; et al. (2001). "Ski7p G protein interacts with the exosome and the Ski complex for 3′-to-5′ mRNA decay in yeast". EMBO Journal. 20 (17): 4684–93. doi:10.1093/emboj/20.17.4684. PMC 125587. PMID 11532933.
- ^ Wang; Lewis, MS; Johnson, AW; et al. (2005). "Domain interactions within the Ski2/3/8 complex and between the Ski complex and Ski7p". RNA. 11 (8): 1291–302. doi:10.1261/rna.2060405. PMC 1370812. PMID 16043509.
- ^ Araki; Takahashi, S; Kobayashi, T; Kajiho, H; Hoshino, S; Katada, T; et al. (2001). "Ski7p G protein interacts with the exosome and the Ski complex for 3′-to-5′ mRNA decay in yeast". EMBO Journal. 20 (17): 4684–93. doi:10.1093/emboj/20.17.4684. PMC 125587. PMID 11532933.
- ^ Halbach, Felix; Reichelt, Peter; Rode, Michaela; Conti, Elena (AUGUST 15, 2013). "The Yeast Ski Complex: Crystal Structure and RNA Channeling to the Exosome Complex". Cell. doi:https://doi.org/10.1016/j.cell.2013.07.017.
{{cite journal}}: Check|doi=value (help); Check date values in:|date=(help); External link in|doi= - ^ Halbach, Felix; Reichelt, Peter; Rode, Michaela; Conti, Elena (AUGUST 15, 2013). "The Yeast Ski Complex: Crystal Structure and RNA Channeling to the Exosome Complex". Cell. doi:https://doi.org/10.1016/j.cell.2013.07.017.
{{cite journal}}: Check|doi=value (help); Check date values in:|date=(help); External link in|doi=
Category:Protein complexes Category:Gene expression Category:Saccharomyces cerevisiae genes
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