User:NBonawitz/sandboxShikimateKinase

shikimate kinase
Identifiers
EC no.	2.7.1.71
CAS no.	9031-51-0
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

Shikimate kinase
Shikimate kinase of Erwinia chrysanthemi
Identifiers
Symbol	SKI
Pfam	PF01202
Pfam clan	CL0023
InterPro	IPR000623
PROSITE	PDOC00868
SCOP2	2shk / SCOPe / SUPFAM
OPM protein	1e6c
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary PDB 1l4yA:11-167 1we2A:11-167 1l4uA:11-167 1u8aA:11-167 2shkB:11-169 1shkA:11-169 1e6cB:11-169 1kagA:13-171 2IYQ 2IYR 2IYS 2IYT 2IYU 2IYV 2IYW 2IYX 2IYY 2IYZ

Shikimate kinase (EC 2.7.1.71) is an enzyme that catalyzes the ATP-dependent phosphorylation of shikimate to form shikimate 3-phosphate. This reaction is the fifth step of the shikimate pathway, which is used by plants and bacteria to synthesize the common precursor of aromatic amino acids and secondary metabolites. The systematic name of this enzyme class is ATP:shikimate 3-phosphotransferase. Other names in common use include shikimate kinase (phosphorylating), and shikimate kinase II.

Background

The shikimate pathway consists of seven enzymatic reactions by which phosphoenolpyruvate and erythrose 4-phosphate are converted to chorismate, the common precursor of the aromatic amino acids phenylalanine, tyrosine, and tryptophan. The aromatic amino acids are used in the synthesis of proteins and, in plants, fungi, and bacteria, give rise to a number of other specialized metabolites, such as phenylpropanoids and alkaloids. Chorismate and several other intermediates of the pathway serve as precursors for a number of other metabolites, such as folates, quinates, and quinones. The four enzymes that precede shikimate kinase in the pathway are DAHP synthase, 3-dehydroquinate synthase, 3-dehydroquinate dehydratase, and shikimate dehydrogenase, and the two that follow it are EPSP synthase and chorismate synthase. The shikimate pathway is not found in humans and other animals, which must obtain the aromatic amino acids from their food.

Activity

The reaction catalyzed by shikimate kinase is shown below:

reaction catalyzed by shikimate kinase

This reaction involves the transfer of a phosphate group from ATP to the 3-hydroxyl group of shikimate. Shikimate kinase thus has two substrates, shikimate and ATP, and two products, shikimate 3-phosphate and ADP.

Structure

A cartoon representation of shikimate kinase from Mycobacterium tuberculosis. α-Helices are shown in red, the central β-sheet in yellow, and loops in green

A space-filling model of shikimate kinase with ADP and shikimate 3-phosphate bound.

As of late 2007, 26 structures have been solved for this class of enzymes, with PDB accession codes 1E6C, 1KAG, 1L4U, 1L4Y, 1SHK, 1U8A, 1VIA, 1WE2, 1ZUH, 1ZUI, 1ZYU, 2DFN, 2DFT, 2G1J, 2G1K, 2IYQ, 2IYR, 2IYS, 2IYT, 2IYU, 2IYV, 2IYW, 2IYX, 2IYY, 2IYZ, and 2SHK.

References

Category:EC 2.7.1 Category:Enzymes of known structure