User:Mortenenghave/sandbox

This is the user sandbox of Mortenenghave. A user sandbox is a subpage of the user's user page. It serves as a testing spot and page development space for the user and is not an encyclopedia article. Create or edit your own sandbox here.

Other sandboxes: Main sandbox | Template sandbox

Finished writing a draft article? Are you ready to request review of it by an experienced editor for possible inclusion in Wikipedia? Submit your draft for review!

Flow Induced Dispersion Analysis (FIDA) is an immobilization-free technology used for characterization and quantification of biomolecular interaction and protein concentration under native conditions^[1]. In the FIDA assay, the size of a ligand (indicator) with affinity to the target analyte is measured. When the indicator interacts with the analyte the apparent size increases and this change in size can be used to determine the analyte concentration and interaction^[1]^[2]. Additionally, the hydrodynamic radius of the analyte-indicator complex is obtained. A FIDA assay is typically completed in minutes and only requires a modest sample consumption of a few µL^[1].

Applications

Quantification of analytes (e.g. proteins, peptides and DNA) in complex solutions (e.g. plasma ^[3])
Determination of
- affinity constants
- binding kinetics
- molecular size (hydrodynamic radius)
- diffusion coefficient

Principle

The FIDA principle is based on measuring the change in the apparent size (diffusivity) of a selective indicator interacting with the analyte molecule^[1]^[2]. The apparent indicator size is measured by Taylor Dispersion Analysis in a capillary under hydrodynamic flow^[4].

References

^ ^a ^b ^c ^d ^e Poulsen, Nicklas N.; Andersen, Nina Z.; Østergaard, Jesper; Zhuang, Guisheng; Petersen, Nickolaj J.; Jensen, Henrik (2015-06-15). "Flow induced dispersion analysis rapidly quantifies proteins in human plasma samples". The Analyst. 140 (13): 4365–4369. doi:10.1039/c5an00697j. ISSN 1364-5528.
^ ^a ^b Jensen, Henrik; Østergaard, Jesper (2010-03-31). "Flow Induced Dispersion Analysis Quantifies Noncovalent Interactions in Nanoliter Samples". Journal of the American Chemical Society. 132 (12): 4070–4071. doi:10.1021/ja100484d. ISSN 0002-7863.
^ Poulsen, Nicklas N.; Pedersen, Morten E.; Østergaard, Jesper; Petersen, Nickolaj J.; Nielsen, Christoffer T.; Heegaard, Niels H. H.; Jensen, Henrik (2016-09-20). "Flow-Induced Dispersion Analysis for Probing Anti-dsDNA Antibody Binding Heterogeneity in Systemic Lupus Erythematosus Patients: Toward a New Approach for Diagnosis and Patient Stratification". Analytical Chemistry. 88 (18): 9056–9061. doi:10.1021/acs.analchem.6b01741. ISSN 0003-2700.
^ Taylor, Sir Geoffrey; S, F. R. (1953-08-25). "Dispersion of soluble matter in solvent flowing slowly through a tube". Proc. R. Soc. Lond. A. 219 (1137): 186–203. doi:10.1098/rspa.1953.0139. ISSN 0080-4630.

[:0-1] Poulsen, Nicklas N.; Andersen, Nina Z.; Østergaard, Jesper; Zhuang, Guisheng; Petersen, Nickolaj J.; Jensen, Henrik (2015-06-15). "Flow induced dispersion analysis rapidly quantifies proteins in human plasma samples". The Analyst. 140 (13): 4365–4369. doi:10.1039/c5an00697j. ISSN 1364-5528.

[:1-2] Jensen, Henrik; Østergaard, Jesper (2010-03-31). "Flow Induced Dispersion Analysis Quantifies Noncovalent Interactions in Nanoliter Samples". Journal of the American Chemical Society. 132 (12): 4070–4071. doi:10.1021/ja100484d. ISSN 0002-7863.

[3] Poulsen, Nicklas N.; Pedersen, Morten E.; Østergaard, Jesper; Petersen, Nickolaj J.; Nielsen, Christoffer T.; Heegaard, Niels H. H.; Jensen, Henrik (2016-09-20). "Flow-Induced Dispersion Analysis for Probing Anti-dsDNA Antibody Binding Heterogeneity in Systemic Lupus Erythematosus Patients: Toward a New Approach for Diagnosis and Patient Stratification". Analytical Chemistry. 88 (18): 9056–9061. doi:10.1021/acs.analchem.6b01741. ISSN 0003-2700.

[4] Taylor, Sir Geoffrey; S, F. R. (1953-08-25). "Dispersion of soluble matter in solvent flowing slowly through a tube". Proc. R. Soc. Lond. A. 219 (1137): 186–203. doi:10.1098/rspa.1953.0139. ISSN 0080-4630.

[1]

[2]

[3]

[4]