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Chromatography (/ˌkroʊməˈtɒɡrəfi/; from Greek χρῶμα chroma which means "color" and γράφειν graphein "to write"[1]) is a laboratory technique for the separation of a mixture of molecules. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture elute at different speeds, causing them to separate. The separation is based on differential partitioning between the mobile and stationary phases. Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus affect the separation.[2]
Chromatography may be preparative or analytical. The purpose of preparative chromatography is to separate the components of a mixture for later use, and is thus a form of purification. Analytical chromatography is done normally with smaller amounts of material and is for establishing the presence or measuring the relative proportions of analytes in a mixture. The two are not mutually exclusive.[3]
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- ^ "chromatography". Online Etymology Dictionary.
- ^ McMurry, John (2011). Organic chemistry: with biological applications (2nd ed.). Belmont, CA: Brooks/Cole. p. 395. ISBN 9780495391470.
- ^ Hostettmann, K; Marston, A; Hostettmann, M (1998). Preparative Chromatography Techniques Applications in Natural Product Isolation (Second ed.). Berlin, Heidelberg: Springer Berlin Heidelberg. p. 50. ISBN 9783662036310.