User:Kagaruno/sandbox
 | This is a user sandbox of Kagaruno. You can use it for testing or practicing edits. This is not the sandbox where you should draft your assigned article for a dashboard.wikiedu.org course. To find the right sandbox for your assignment, visit your Dashboard course page and follow the Sandbox Draft link for your assigned article in the My Articles section. |
Alkaline Phosphatase is a Zinc containing dimeric enzyme with the MW: 86,000 kDa. It is heat stable and its function is to remove phosphate groups from phosphorylated compounds facilitating transport across membranes and providing the cell with a source of inorganic phosphate. Alkaline Phosphatase in E.coli is located in the periplasmic space and we can thus, selectively release it with a technique that weakens the cell wall and releases the proteins. Due to the location of the enzyme, and the protein layout of the enzyme the enzyme is in solution with a small amount of proteins than there are in another portion of the cell.