User:JustinCHEM446/Outline
Protein Adsorption
Trevor Shepherd, Justin Hui, Jessica Cho
Methods to investigate protein adsorption
[edit]1. Fluorescent labeling / Radio labeling
2. Atomic Force Microscopy (AFM)
- Measures the force of adhesion between a protein-coated probe and the arrayed polymers.
3. Ceramics: 2D PAGE
4. Surface-Matrix Assisted Laser Desorption Ionization Mass Spectrometry (Surface-MALDIMS)[1]
- Instrument uses a pulsed UV laser for protein ionization and delayed extraction combined with time-of-flight analysis for mass separation
Metals
[edit]Relevance
[edit]1. Sterilization of implants and medical devices (reduced risk of disease transmission
- a. Surgical tool design
2. Reduced risk of corrosion
3. Engineer devices to have a lower risk of rejection after implantation
- a. Incorporating native proteins will reduce the host immune and inflammatory response
Chemical composition
[edit]1. Crystalline structure
- a. Crystal packing exposes potential metal-ion-dependent adhesion site (MIDAS) on one edge of the beta-sandwich and may serve as a binding site for collagens and other ligands. [2]
Coordination number
[edit]IMFs
[edit]Polymers
[edit]Chemical composition
[edit]Coordination number
[edit]IMFs
[edit]1. Dispersion
2. Dipole-dipole
3. Hydrogen Bonding
Ceramics
[edit]Chemical composition
[edit]Coordination number
[edit]IMFs
[edit]Effect of surface properties on adsorption
[edit]Surface wettability
[edit]1. Contact angle
2. Surface energy