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Multiview light sheet microscopy, also called multiview selective-plane illumination microscope (MuVi-SPIMIM), is a light sheet technology that utilizes more than one illumination or detection arms to realize imaging samples from different perspective. Typically, multi-view microscopy can be used to diminish the loss of signal induced by the absorption or the scattering effects^[1], thus this technology enables higher axial resolution and bigger field of view^[2]. The common multi-view light sheet microscopy includes two illumination one detection^[1], one illumination two detection, and two illumination two detection arms^[2].

Advantages

Compared to mono-view imaging without spinning, the imaging quality of which is not high due to extinction, the multiview imaging contains more spatial information of the sample. Compared to mono-view imaging with spinning, the multi-view imaging reduces the needs to spin the sample, thus it is quicker, considering the ratio of time of mechanical spinning and time of imaging is high. Multiview light-sheet microscopy eliminates the need for specimen-specific data fusion algorithms, streamlines image post-processing, and thereby eases data handling and storage.^[3]

Principles

If the sample is absorber and two illumination arms are used, the two colliding beams can compensate so that the image will not be dim on one side due to absorption of light. If two illumination and two detection arms are used, by setting different open time for two illumination arms, the 2-2 illu-detection can enable four imaging branches. By fusing the 4 branches, the spatial resolution will be higher as the two symmetrical illumination sources decrease the extinction of signal on the sheet plane when the sheet travels inside the sample.

^ ^a ^b Huisken, Jan; Didier Y. R. Stainier (September 2007). "Even Fluorescence Excitation by Multidirectional Selective Plane Illumination Microscopy (MSPIM)". Optics Letters. 32 (17). doi:10.1364/OL.32.002608.
^ ^a ^b Krzic, Uros; Stefan Gunther; Timothy E. Saunders; Sebastian J. Streichan; Lars Hufnagel (July 2012). "Multiview Light-Sheet Microscope for Rapid in Toto Imaging". Nature Methods. 9 (7). doi:10.1038/nmeth.2064. Cite error: The named reference "doi.org/10.1038/nmeth.2064" was defined multiple times with different content (see the help page).
^ Medeiros, Gustavo de; Nils Norlin; Stefan Gunther; Marvin Albert; Laura Panavaite; Ulla-Maj Fiuza; Francesca Peri; Takashi Hiiragi; Uros Krzic; Lars Hufnagel (November 2015). "Confocal Multiview Light-Sheet Microscopy". Nature Commnications. 67. doi:10.1038/ncomms9881.

[doi.org/10.1364/OL.32.002608-1] Huisken, Jan; Didier Y. R. Stainier (September 2007). "Even Fluorescence Excitation by Multidirectional Selective Plane Illumination Microscopy (MSPIM)". Optics Letters. 32 (17). doi:10.1364/OL.32.002608.

[doi.org/10.1038/nmeth.2064-2] Krzic, Uros; Stefan Gunther; Timothy E. Saunders; Sebastian J. Streichan; Lars Hufnagel (July 2012). "Multiview Light-Sheet Microscope for Rapid in Toto Imaging". Nature Methods. 9 (7). doi:10.1038/nmeth.2064. Cite error: The named reference "doi.org/10.1038/nmeth.2064" was defined multiple times with different content (see the help page).

[doi.org/10.1038/ncomms9881-3] Medeiros, Gustavo de; Nils Norlin; Stefan Gunther; Marvin Albert; Laura Panavaite; Ulla-Maj Fiuza; Francesca Peri; Takashi Hiiragi; Uros Krzic; Lars Hufnagel (November 2015). "Confocal Multiview Light-Sheet Microscopy". Nature Commnications. 67. doi:10.1038/ncomms9881.

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