User:Danica319/sandbox/cell mechanics

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Eukaryotic Cell Mechanics

Eukaryotic cells are cells that consist of membrane-bound organelles, a membrane-bound nucleus, and more than one linear chromosome. Being much more complex than prokaryotic cells, cells without a true nucleus, eukaryotes must protect its organelles from outside forces.

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Animal Cell Mechanics

Because animal cells do not have cell walls to protect them like plant cells, they require other specialized structures to sustain external mechanical forces. All animal cells are encased within a cell membrane made of a thin lipid bilayer that protects the cell from exposure to the outside environment. Using receptors composed of protein structures, the cell membrane is able to let selected molecules within the cell. Inside the cell membrane includes the cytoplasm, which contains the cytoskeleton. A network of filamentous proteins including microtubules, intermediate filaments, and actin filaments makes up the cytoskeleton and helps maintain the cell’s shape. By working together, the three types of polymers can organize themselves to counter the applied external forces and resist deformation. However, there are differences between the three polymers.

The primary structural component of the cytoskeleton is actin filaments. Being the narrowest with a diameter of 7nm and most flexible out of the three types of polymers, actin filaments are typically found at the very edge of the cytoplasm in animal cells. Formed by the linking of polymers of a protein called actin, they help give cells shape and structure and are able to transport protein packages and organelles. Furthermore, actin filaments have the ability to be assembled and disassembled quickly, allowing them to take part in cell mobility.

On the other hand, intermediate filaments are more permanent structures with a diameter of 8 to 10 nm. Composed of numerous fibrous protein strands wound together, intermediate proteins’ main role is bearing tension and retaining the shape and structure of the cell by securing the nucleus and other organelles in their designated areas.

The largest cytoskeletal structure of the three types of polymers is the microtubules with a diameter of 25nm . Unlike actin filaments, microtubules are stiff, hollow structures that radiate outwards from the microtubule organizing center (MTOC). Comprised of tubulin proteins, microtubules are dynamic structures that allows them to shrink or grow with the addition or removal of tubulin proteins. In terms of cell mechanics, microtubules’ main purpose is to resist compressive cellular forces and act as a transportation system for motor proteins.

Experimental Methods for Measuring Cell Mechanics

Because cells are tiny, soft objects that must be measured differently than materials like metal, plastic, and glass, new techniques have been developed for the accurate measurement of cell mechanics. The variety of techniques can be divided into two categories: force application techniques and force sensing techniques .

Force Application Techniques

Force application techniques uses the cell’s response of deformation to force applied onto the cell as a way to measure traction forces. There are several different types of force application techniques including:

1. Micropipette aspiration uses applied suction pressure with a small diameter glass pipet. The measurement of the length of aspiration caused by the suction pressure can reveal several cell mechanical properties.
2. Cantilever manipulation operates through an magnetic, electrical, or mechanical interaction between a probe and the surface of the cell that gives off a signal that can be used to measure mechanical properties.
3. Optical techniques involves the usage of trapped photons to manipulate cells. The photons will change in direction based on the cell’s refractive index, which will cause a change in momentum, leading to a force applied upon the cell.
4. Mechanical techniques utilizes the incorporation of ferromagnetic beads into the cell or attached to specific receptors on the cell. When a magnetic force is applied, the stretch of the membrane can be measured to calculate mechanical properties.
5. Substrate strain measures elasticity through stretching the cell. The elasticity of the cell provides information that can determine motility and adhesion.
6. Compression requires the usage of applied pressure onto the entire cell. By calculating the changes of the cell’s shape, compression is a way to measure mechanical responses to force.
7. Flow Techniques uses Reynold’s number, a dimensionless number in fluid mechanics, to distinguish whether the cell is subject to laminar, transitional, or turbulent flow.

Force Sensing Techniques

1. Wrinkling membranes requires putting the cell into a flexible silicon envelope. As the cell contracts, the magnitude of the forces can be estimated by utilizing the length and number of wrinkles.
2. Traction force microscopy detects deformations through comparison of images the movement of fluorescent beads that have been adhered to the cell.
3. Cantilever sensing can detect surface stresses with the attachment of micromechanical beams on one end of the cell.
4. Bioreactors allow the measurement of multicellular forces in a three-dimensional system, while external forces are applied at the same time. This enables better results and more accurate data from complex experiments.