User:Amarchais/CsfG RNA

CsfG in *Bacillus subtilis*. Highly conserved bases are in red.

CsfG is a non-coding RNA that is transcribed only during sporulation, specifically in the forespore. It was discovered in Bacillus subtilis using a large scale computational screening based on phylogenetic profiling.^[1] In vivo analysis confirmed that csfg transcription is regulated by SigG and SigF.^[2] In competition with a wild-type strain, the csfG deleted strain show a loss of about 29 % at each cycle of growth, sporulation and germination, presumably as a consequence of a slight germination delay of the mutant spores. CsfG is present in many endospore formers, mostly Bacilli and some Clostridia, whereas it is absent from bacteria that do not produce endospores.This locus was originally reported on the reverse strand as a cis-regulatory element under the name "ylbH leader".^[3] CsfG completely overlaps the ylbH element, with a 28-nt extension on the 5' end of CsfG (in B. subtilis).

References

^ Marchais A, Naville M, Bohn C, Bouloc P, Gautheret D (April 2009). "Single-pass classification of all noncoding sequences in a bacterial genome using phylogenetic profiles". Genome Res. 19 (6): 1084–1092. doi:10.1101/gr.089714.108. PMC 2694484. PMID 19237465.{{cite journal}}: CS1 maint: date and year (link) CS1 maint: multiple names: authors list (link)
^ Marchais A, Duperrier S, Durand S, Gautheret D, Stragier P (May 2011). "CsfG, a sporulation-specific, small non-coding RNA highly conserved in endospore formers". RNA Biol. 8 (3): 358–364. doi:10.4161/rna.8.3.14998. PMC 3218505. PMID 21532344.{{cite journal}}: CS1 maint: date and year (link) CS1 maint: multiple names: authors list (link)
^ Barrick JE, Corbino KA, Winkler WC; et al. (April 2004). "New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control". Proc. Natl. Acad. Sci. U.S.A. 101 (17): 6421–6. doi:10.1073/pnas.0308014101. PMC 404060. PMID 15096624. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: date and year (link) CS1 maint: multiple names: authors list (link)

External links

CsfG Alignment [1]

[pmid19237465-1] Marchais A, Naville M, Bohn C, Bouloc P, Gautheret D (April 2009). "Single-pass classification of all noncoding sequences in a bacterial genome using phylogenetic profiles". Genome Res. 19 (6): 1084–1092. doi:10.1101/gr.089714.108. PMC 2694484. PMID 19237465.{{cite journal}}: CS1 maint: date and year (link) CS1 maint: multiple names: authors list (link)

[pmid21532344-2] Marchais A, Duperrier S, Durand S, Gautheret D, Stragier P (May 2011). "CsfG, a sporulation-specific, small non-coding RNA highly conserved in endospore formers". RNA Biol. 8 (3): 358–364. doi:10.4161/rna.8.3.14998. PMC 3218505. PMID 21532344.{{cite journal}}: CS1 maint: date and year (link) CS1 maint: multiple names: authors list (link)

[pmid15096624-3] Barrick JE, Corbino KA, Winkler WC; et al. (April 2004). "New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control". Proc. Natl. Acad. Sci. U.S.A. 101 (17): 6421–6. doi:10.1073/pnas.0308014101. PMC 404060. PMID 15096624. {{cite journal}}: Explicit use of et al. in: |author= (help)CS1 maint: date and year (link) CS1 maint: multiple names: authors list (link)

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