Jump to content

UBE2V2

From Wikipedia, the free encyclopedia
UBE2V2
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesUBE2V2, DDVIT1, DDVit-1, EDAF-1, EDPF-1, EDPF1, MMS2, UEV-2, UEV2, ubiquitin conjugating enzyme E2 V2
External IDsOMIM: 603001; MGI: 1917870; HomoloGene: 55739; GeneCards: UBE2V2; OMA:UBE2V2 - orthologs
Orthologs
SpeciesHumanMouse
Entrez

7336

70620

Ensembl

ENSG00000169139

ENSMUSG00000022674

UniProt

Q15819

Q9D2M8

RefSeq (mRNA)

NM_003350

NM_001159351
NM_023585

RefSeq (protein)

NP_003341

NP_001152823
NP_076074

Location (UCSC)Chr 8: 48.01 – 48.06 MbChr 16: 15.37 – 15.41 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Ubiquitin-conjugating enzyme E2 variant 2 is a protein that in humans is encoded by the UBE2V2 gene.[5][6] Ubiquitin-conjugating enzyme E2 variant proteins constitute a distinct subfamily within the E2 protein family.

Structure

[edit]

UBE2V2 has sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. The protein encoded by this gene also shares homology with ubiquitin-conjugating enzyme E2 variant 1 and yeast MMS2 gene product.[7]

Function

[edit]

UBE2V2 has also been implicated as an intracellular sensor of reactive electrophilic species, which are present in high levels during periods of pathogenic and/or environmental stress.[8] The C69 residue of UBE2V2 is capable of binding with various RES. It has been shown that binding of RES to UBE2V2 promotes UBE2V2-mediated activation of Ube2N, another E2 protein that complexes with UBE2V2. Activated Ube2N has been shown to play a major role in promoting DNA-damage responses. Thus, UBE2V2 may promote genome integrity by directly sensing RES and effecting DNA damage responses.[9] It may also be involved in the differentiation of monocytes and enterocytes.[7]

Interactions

[edit]

UBE2V2 has been shown to interact with HLTF.[10] Although UBE2V2 itself lacks ubiquitin-conjugating activity, it can interact with different Ubiquitin-conjugating enzymes to facilitate their catalytic activities.[11] For instance, UBE2V2 can complex with UBE2N to form a heterodimer capable of synthesizing Lys-63 linked polyubiquitin chains.[12] UBE2V2 may facilitate UBE2N activity by coordinating UBE2N's positioning to promote ubiquitin chain formation specifically at Lys-63, as the ubiquitin molecule has multiple potential Lysine binding sites.[13] Similarly, it has been shown that UBE2V2 interact with the ubiquitin-conjugating enzyme, Ubc13, to induce Ubc13 to adopt an active conformation that can create Lys-63 polyubiquitin chains on various substrates.[14]

Addition of Lys-63 polyubiquitin chains to intracellular targets is distinct from the canonical Lys-48 polyubiquitin chains in that Lys-63 chains do not mediate proteasomal degradation of its substrate.[15] Although their function remains poorly characterized, Lys-63 chains have been shown to regulate signaling pathways by either activating or inhibiting its target protein function.[16] For example, TRIM5alpha restriction of retroviral reverse-transcription is dependent on UBE2V2/UBE2N-mediated poly-ubiquitination.[17] UBE2V2 has been shown to regulate TRIM21 antiviral activity in an analogous manner.[18]

References

[edit]
  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000169139 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000022674 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Sancho E, Vilá MR, Sánchez-Pulido L, Lozano JJ, Paciucci R, Nadal M, Fox M, Harvey C, Bercovich B, Loukili N, Ciechanover A, Lin SL, Sanz F, Estivill X, Valencia A, Thomson TM (January 1998). "Role of UEV-1, an inactive variant of the E2 ubiquitin-conjugating enzymes, in in vitro differentiation and cell cycle behavior of HT-29-M6 intestinal mucosecretory cells". Molecular and Cellular Biology. 18 (1): 576–89. doi:10.1128/mcb.18.1.576. PMC 121525. PMID 9418904.
  6. ^ Fritsche J, Rehli M, Krause SW, Andreesen R, Kreutz M (June 1997). "Molecular cloning of a 1alpha,25-dihydroxyvitamin D3-inducible transcript (DDVit 1) in human blood monocytes". Biochemical and Biophysical Research Communications. 235 (2): 407–12. doi:10.1006/bbrc.1997.6798. PMID 9199207.
  7. ^ a b "Entrez Gene: UBE2V2 ubiquitin-conjugating enzyme E2 variant 2".
  8. ^ Farmer EE, Davoine C (August 2007). "Reactive electrophile species". Current Opinion in Plant Biology. 10 (4): 380–6. doi:10.1016/j.pbi.2007.04.019. PMID 17646124.
  9. ^ Zhao Y, Long MJ, Wang Y, Zhang S, Aye Y (February 2018). "Ube2V2 Is a Rosetta Stone Bridging Redox and Ubiquitin Codes, Coordinating DNA Damage Responses". ACS Central Science. 4 (2): 246–259. doi:10.1021/acscentsci.7b00556. PMC 5833000. PMID 29532025.
  10. ^ Unk I, Hajdú I, Fátyol K, Hurwitz J, Yoon JH, Prakash L, Prakash S, Haracska L (March 2008). "Human HLTF functions as a ubiquitin ligase for proliferating cell nuclear antigen polyubiquitination". Proceedings of the National Academy of Sciences of the United States of America. 105 (10): 3768–73. Bibcode:2008PNAS..105.3768U. doi:10.1073/pnas.0800563105. PMC 2268824. PMID 18316726.
  11. ^ Stewart MD, Ritterhoff T, Klevit RE, Brzovic PS (April 2016). "E2 enzymes: more than just middle men". Cell Research. 26 (4): 423–40. doi:10.1038/cr.2016.35. PMC 4822130. PMID 27002219.
  12. ^ Fletcher AJ, Christensen DE, Nelson C, Tan CP, Schaller T, Lehner PJ, Sundquist WI, Towers GJ (August 2015). "TRIM5α requires Ube2W to anchor Lys63-linked ubiquitin chains and restrict reverse transcription". The EMBO Journal. 34 (15): 2078–95. doi:10.15252/embj.201490361. PMC 4551353. PMID 26101372.
  13. ^ Eddins MJ, Carlile CM, Gomez KM, Pickart CM, Wolberger C (October 2006). "Mms2-Ubc13 covalently bound to ubiquitin reveals the structural basis of linkage-specific polyubiquitin chain formation". Nature Structural & Molecular Biology. 13 (10): 915–20. doi:10.1038/nsmb1148. PMID 16980971. S2CID 6757737.
  14. ^ Branigan E, Plechanovová A, Jaffray EG, Naismith JH, Hay RT (August 2015). "Structural basis for the RING-catalyzed synthesis of K63-linked ubiquitin chains". Nature Structural & Molecular Biology. 22 (8): 597–602. doi:10.1038/nsmb.3052. PMC 4529489. PMID 26148049.
  15. ^ Fletcher AJ, Christensen DE, Nelson C, Tan CP, Schaller T, Lehner PJ, Sundquist WI, Towers GJ (August 2015). "TRIM5α requires Ube2W to anchor Lys63-linked ubiquitin chains and restrict reverse transcription". The EMBO Journal. 34 (15): 2078–95. doi:10.15252/embj.201490361. PMC 4551353. PMID 26101372.
  16. ^ "UBE2N - Ubiquitin-conjugating enzyme E2 N - Homo sapiens (Human) - UBE2N gene & protein". www.uniprot.org. Retrieved 2018-04-06.
  17. ^ Fletcher AJ, Christensen DE, Nelson C, Tan CP, Schaller T, Lehner PJ, Sundquist WI, Towers GJ (August 2015). "TRIM5α requires Ube2W to anchor Lys63-linked ubiquitin chains and restrict reverse transcription". The EMBO Journal. 34 (15): 2078–95. doi:10.15252/embj.201490361. PMC 4551353. PMID 26101372.
  18. ^ Fletcher AJ, Mallery DL, Watkinson RE, Dickson CF, James LC (August 2015). "Sequential ubiquitination and deubiquitination enzymes synchronize the dual sensor and effector functions of TRIM21". Proceedings of the National Academy of Sciences of the United States of America. 112 (32): 10014–9. Bibcode:2015PNAS..11210014F. doi:10.1073/pnas.1507534112. PMC 4538650. PMID 26150489.

Further reading

[edit]
Stub icon

This article on a gene on human chromosome 8 is a stub. You can help Wikipedia by expanding it.

Retrieved from "https://en.wikipedia.org/w/index.php?title=UBE2V2&oldid=1081691081"