Talk:Stable nucleic acid lipid particle

Peer Review from Group 81C

Hi All. My name is Meredith and my group (81C) has been assigned to peer-review your wikipedia article. It looks like you have made some great progress on the article so far. My fellow group member Jea has made some suggestions for the backgound section of your article. I'd like to say that I really like the addition of the image. It was really helpful to visualize exactly what an SNALP was. From the image it looks like the lipid by-layer that encapsulates the siRNA is in the structure of a liposome. It might be beneficial to add some information about what a liposome is or a link to the Liposome wikipedia page in case people reading the article are curious about this molecular structure. Here are a couple other suggestions/questions/comments I had when reading your article:

• Add a link to the RISC wikipedia page
• The second sentence of the second paragraph of the background section currently reads "Pattern recognition receptors(PRRs), which can be grouped as endocytic PRRs or signaling PRRs, are expressed in all cells of he innate immune system." Should the "he" in this sentence be "the"?
• Have there been any applications of SNALPs used as cancer treatments or for genetic diseases?

The progress on the article is looking great! MHN2785 (talk) 03:37, 29 November 2012 (UTC)

Suggestion on Background Section

Hey guys, Jea from Group 81C here. Nice start on your article. In your intro, you state that SNALPs are microscopic particles, but my understanding is that particle sizes larger than atoms or small molecules do not classify as microscopic. Wondering if it could be classified as nanoparticles as this glossary states?

http://www.nature.com/nrg/journal/v12/n5/glossary/nrg2968.html#df9

Your Background section has alot of good info but lacks explanation on some of the concepts that you introduce. For instance, I like how you begin the article with the breakdown of RNA interference and how siRNA can be used to achieve regulation, but your sudden jump into siRNA delivery in your last sentence without explaining what it is creates discontiuity. Maybe supplement background on siRNA delivery system? The following ariticle has good material you can use under the heading "siRNA delivery options."

http://www.nature.com/nrg/journal/v12/n5/full/nrg2968.html - Jpark623 (talk) 16:25, 12 November 2012 (UTC)

Hello Jea, from my understanding a microscopic element is one that is too small to be seen by the naked eye and need the use of a microscope to be visible. SNALPs are extremely small, smaller than the visible light spectrum, and therefore are only visible through the use of electron microscopes. Check out this article for some pretty neat images: http://pubs.acs.org/doi/full/10.1021/ja302930b

Nanoparticle are merely a subcategory of particles. Nanoparticles usually range from 1 to about 100 nanometers which would correctly categorize SNALPs as nanoparticles. The keyword 'particle' was used in this context to be consistent with the SNALP terminology and acronym.

We plan to expand on the article in a number of ways. Within the next couple weeks we hope to add substantial content to the Applications section as well as other areas of the article to help aid in the reader's understanding. To see a break down of what we plan to accomplish check out our group page. ShanSabri (talk) 17:37, 12 November 2012 (UTC)

However you'd like to call it is up to you. Just wanted to provided feedback per our assignment guidelines. I hope I didn't come off as being critical... - Jpark623 (talk) 21:00, 12 November 2012 (UTC)

Absolutely not! That's the whole point of peer-review. Critiques are always needed. Uplifted1 and I will most definitely keep these suggestions in mind. ShanSabri (talk) 21:16, 12 November 2012 (UTC)

Final week mini-review

Hi, guys. Here's a final-week mini-review of your page. It is just a few random notes, that I jotted down while skimming the page, and is not intended as a comprehensive review.

• Figures. Please see some suggestions here, under "Figures and Images".
• "endocytic PRRs" and "signaling PRRs" are redlinks, which means the pages they link to do not exist. You should change these to normal text, since it's not likely that these specific pages will be created soon (they are already covered in the PRR page).
• Why are there redlinks in your references? Did you use User:Diberri's Wikipedia template filling tool (instructions) that was described on the project page? Please clean these up.
• In the first paragraph, you switch abruptly from describing something that "occurs naturally" to a laboratory technique. I think you could start talking about the lab technique in a separate paragraph, and make it clear that that's what you are doing. For example, do SNALPs themselves occur naturally? After skimming through this, I'm still not sure.
• The second paragraph of the introduction is too technical and difficult to understand. You need to introduce things more slowly. This goes hand-in-hand with my previous comment. You are talking about a lab technique. Guide the reader step-by-step. There is a problem applying this technique in living systems (avoid jargony "in vivo" unless you introduce it first). Why is it a problem? What is the mechanims? Go slow, and introduce technical terms before you use them. In this regard, the detailed section, "Development of SNALP delivery of siRNA", is actually easier to read than the introduction -- but it should be the other way around.
• In "Development of SNALP delivery of siRNA", in the wikitext, you wrote, "[[sense (molecular biology)]]". This should be "[[sense (molecular biology)|sense]]", so that the "molecular biology" doesn't appear in the text, like this: sense.
• I think the text box under "Zaire Ebola virus (ZEBOV)", that provides the actual sequences of the siRNAs, is too much detail, and should be removed.
• Besides the ZEBOV application, were there any other applications of these SNALPs?
• The article is still pretty short. Is there more content you could add?

Klortho (talk) 19:17, 9 December 2012 (UTC)