Talk:SH-SY5Y

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Now I'm studying cell biology at University (admittedly at the first level), and I can understand about 15 words in that article. Needs a bit of a fix, I think. — riana_dzast a _{wreak havoc|damage report} 08:55, 7 September 2006 (UTC)[reply]

How can a tumor cell derived from progenitor cells of neuronal tissue exert a blood type?

I just stumbled across this article, having worked with these cells for several years... and it's terrible. I plan on overhauling it when I get the chance. Little information is actually cited, and most of what isn't is wrong. The technical stuff doesn't really need to be there, at least not in that level of detail, and much of the protocol that is there is completely context-specific, making several implicit assumptions as to the application. EPiet (talk) 18:08, 22 July 2009 (UTC)[reply]

I have improved the History section. I changed "third generation" to the more appropriate "thrice cloned". Generations refer to population doublings, not subcultivated lines. The description of cloning was also very incorrect. Cloning refers to making genetic copies, which in this case simply means selecting single cells and allowing them divide, producing a genetically identical population of cells (a "clonal line"). The original text in this section explained the basic process of somatic nuclear transfer, which is associated with cloning entire organisms from gametes and is decidedly not how cell lines are cloned. Also, SK-N-SH cells were derived from a metastatic site in bone marrow, but that doesn't make it a "bone tumor", which means a tumor that developed from bone cells, not just one in bone. The very fact that it was a neuroblastoma precludes it from having been a bone tumor. I didn't find an immediately apparent reference for the blood typing claim, so I removed it for now as it was uncited. I also added the original citation for SH-SY5Y and the original paper for SK-N-SH, which describes the case history of the neuroblastoma. EPiet (talk) 21:39, 22 July 2009 (UTC)[reply]