Talk:RNA polymerase IV

Wiki Education Foundation-supported course assignment

This article was the subject of a Wiki Education Foundation-supported course assignment, between 31 August 2020 and 8 December 2020. Further details are available on the course page. Student editor(s): Yalmanso, NWJchem455, Mavs123, Schamdin, SultanElhaj. Peer reviewers: LeMelleKH2.

Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 02:55, 18 January 2022 (UTC)

MLibrarian Peer-review

I like how you expanded on the topic. I would suggest hyperlinking some of the concepts, like DNA or siRNA to the existing Wiki pages. For references that are currently inside the brackets - please cite them in the references section. Would be great to have some figure! When use abbreviations, either hyperlink them to existing articles, or spell them out the first time you are using them. MLibrarian (talk) 16:19, 14 October 2020 (UTC)

Peer-Review

I have a lot of similar comments to the MLibrarian, but some things that I think should be explained (or, if possible, you should link to an existing Wikipedia page) include: ROS1 demethylase, rDNA, dsRNA, DCL3, Blast, etc. I'm unfamiliar with a lot of the proteins listed, so adding their full name or linking to another Wikipedia page would make things more clear.

I also think that figures would be a good idea. You could include the structure of RNA PolIV, or something that shows the mechanism/how it interacts with other proteins/enzymes.

Overall, I think the wording you chose is very clear, so once you fix the slight issues listed above, this will be a really good page. Spensley15 (talk) 14:09, 14 October 2020 (EDT)

Topic Peer Review 1

Good Job! You really added some important information to this wiki topic! Here are some general comments I have:

In the discovery section, perhaps explain what duplication events are or link it to its own wiki topic. This topic seems important to the discovery and it’s important for people to understand what it means.

-- For the structure section, as they mentioned before, since you do lots of comparison of the different polymerase structures, figures (with labels of the different domains) would be super helpful. And since you mention the 12 subunits of RNA Poly IV, maybe you could explain what some of the specific subunits do.

-- For the mechanism section, I believe that there are many steps and that a figure or diagram or link to a diagram process would be helpful. It could be easier to follow with a visual.

-- For the regulation section, perhaps explain more about how plants regulate the production of SHH1. Like does the plant naturally need to keep levels of this protein to have siRNAs available to fight off foreign viruses? Also, perhaps explain a little more/clarify what histone repressive modifications are. So I think it means when this protein is mutated and cannot bind the histone repressive modifications then siRNAs will stop being produced? It could be a little more clear or quicker to understand if you explain histone repressive modifications. -- Good work overall, and great content!! Timothy.li2021 (talk) —Preceding undated comment added 05:59, 15 October 2020 (UTC)

Topic Peer Review 2

Hello! I just want to say you did a great job with this initial draft and want to leave a few comments and suggestions:

You just try to make your own graphic or cite one from a research database that helps us visualize the structural features of RNA Polymerase IV

I would organize the thoughts in the "original content" section so that sentences flow better. In that same section, I would also clarify what "DCL3" means. For the "structure" section, I would remove the phrase "yet are not required for DNA methylation" because it seems off topic and makes the sentence kinda long. The regulation and mechanism sections look pretty good but I would split the sections into smaller paragraphs so the content is more digestible. You should provide links to some of the terms such "RNA-dependent RNA polymerase" and "DNA Methylation" with existing wikipedia pages so it provides readers with an easier access. Overall this is great, just explain some terms a little more and try to make things as easy to read as possible!

klambaKlamba (talk)

Topic Peer Review 3

Hey all! Really really great job on this! Especially compared to the original article, you have basically forklifted this page, and there's a lot of good stuff on here. Like the others, I do have a few suggestions.

--Like the above review, I do think a visual aid will help this article a ton. It is a lot of text, and having some image to look at can help ground some of that information, so the reader does not feel overwhelmed.

--In Original Content, I get a little lost when you mention ROS1, so I would recommend linking to a ROS1 page, or explaining what that is more clearly.

--In Discovery, I think you can dive a bit deeper into the implications of the analogous structures between RNAP II and RDP1/2. I think that's a fascinating and important point, but it feels a bit glossed over.

--For structure, I think providing a quick blurb about where RNA Poly. II comes from, and its differences. I like that you compare Poly. IV to another Polymerase, but as an average reader, I may not know what Poly. II is used for. Because they are similar, perhaps they serve analogous purposes as well, which can help contextualize its structure. I would also explain why CTDs are necessary for the siRNA production, and also why they aren't for DNA Methylation; I feel like without that, the sentence is just there, without the proper context to justify having it (and I definitely think it's good info to be there!).

Really great work!! LeMelleKH2 (talk) 14:29, 15 October 2020 (UTC)

Meredith's comments

This reads really well so far, and you have clearly expounded a lot on what had previously been posted. I looked over the other comments and most of what I would comment on was covered. But I do want to emphasize a few things. I think a figure of how RNA Pol IV is arranged or how it works could be really beneficial. It could be right at the top next to your lead paragraph - which you also need. It is just important to give the average reader a brief overview oof thee most relevant points about the topic. Also, subheadings would be really beneficial throughout each of your sections. Make sure to keep your language straight forward, as encyclopedia articles are written. Overall, a very informative read. Good job so far. Purchalm (talk) 16:10, 15 October 2020 (UTC)

Response to Peer Review Comments

Thank you all for the tremendous feedback you have given us! Since reading your comments, we have been working to fix the issues identified in order to finalize our page. For our first order of business, we streamlined a lot of the language used and defined unfamiliar terminology in order to make our page a bit more accessible and understandable for all audiences. By adding an introduction section, we were able to give a short summary of our page that was easy to read. Furthermore, we added many links to other Wikipedia pages throughout our page in order to add context for anyone willing to explore the topics mentioned. We added two figures: one of the structure of RNA Polymerase IV and another of the mechanism by which it silences DNA, in order to create a visualization of our word descriptions that made the text easier to digest. We also added subheadings to our discovery section to give each paragraph a unifying theme.

Mavs123 (talk) 03:34, 1 December 2020 (UTC)