Talk:Pribnow box

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Umm, the fiction section... in Neon Genesis, I believe that they're actually referring to the Pribnow Box itself, I don't think that it's "a sort of jargon" at all. I mean the context is that of an alien entity attempting to hack the HQ's control computer, and they discover that the hack is originating from the Prinbow Box. Isn't that pretty closely analagous to the action of RNA polymerase in a viral attack? Not to mention the entire film is an allegory, so I think that it's pretty likely that this was the auter's intention. Anyone have an objection to changing it? [05/04/2007]

I'm going to double my efforts to get around to watching NGE based solely on the above comment. Meanwhile, how in-line are the Pribnow box probabilities with current research/is there somewhere we can easily check? I would assume these probabilities will change as sequences (specifically Pribnow seqs) roll in, regardless of indicative trends in model organisms... Geno-Supremo (talk) 08:36, 20 May 2009 (UTC)[reply]

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GautamGhosh1996 (talk) 22:35, 5 September 2018 (UTC)[reply]

First use of the phrase

Thought I'd leave this here for anyone who might want to use it to expand the article. I do believe I have found the first published use of the phrase "Pribnow box", here. I was actually trying to figure out exactly why "box" is used in the name of so many sequences. The author of that paper highlighted homologous regions of promoters in his figures by drawing a literal box around the conserved sequence that Pribnow had noticed, thus the Pribnow box. Someguy1221 (talk) 09:08, 9 March 2019 (UTC)[reply]

Interesting, although that paper's paywalled to hell and back; can you give the actual details? DS (talk) 14:20, 9 March 2019 (UTC)[reply]

Sure. Gilbert, Walter. Starting and Stopping Sequences for the RNA Polymerase. Cold Spring Harbor Monograph Archive, North America, 06 Jan. 1976. Available at: http://cshmonographs.org/index.php/monographs/article/view/3898/3115. Date accessed: 11 Mar. 2019.

In figure 1 Gilbert presents five promoter fragment sequences that had been published (four phage and one bacterial) in Schaller (1975), Sugimoto (1975), Pribnow (two papers, both in 1975) and Walz (1975). This was prior to Sanger sequencing, by the way. In the figure legend, "A seven-base region of approximate homology is boxed". Further down the paper, "Pribnow (1975a) observed that there is a region of approximate homology in all of the sequences... In Figure 1, this region is boxed..."

Gilbert then discusses experiments with mutant promoters by several groups. He highlights one of these, a 2-bp replacement in the lac operon promoter, which enhances expression. In his own words, "This produces a better fit within the "Pribnow Box."" [Quotation marks in the original.] One more time he refers to this region as "the boxed area" and twice more as "Pribnow Box", but without quotation marks. Of the three other figures in the monograph, two show sequences that have Pribnow Boxes, and these are again boxed.

The rest of the paper discusses several other regions though to be potentially conserved with varying levels of supporting evidence, along with general DNA features found to be important for transcription, and the theory of why all of these features, Pribnow Box included, are important. Someguy1221 (talk) 22:59, 11 March 2019 (UTC)[reply]