Talk:Phase contrast microscope

merger with "phase contrast microscopy"

I think the two articles should be merged.--Kramer 23:52, 7 June 2007 (UTC)[reply]

I do too. But into "phase contrast microscopy" (but I guess that's obvious).Michael zh 13:05, 16 July 2007 (UTC)[reply]

In favour too Ramoul 14:29, 13 August 2007 (UTC)[reply]

yes, the phase contrast microscope section is too small to be of use on its own and would be virtually identical to the microscopy section.

No because then the phase contrast microscope page will be too long and tedious to search through to find out about what are the disadvantages of the microscopy for example- Chandni Vishrolia

Support - They should be merged to phase contrast microscopy. Nmnogueira 15:39, 29 September 2007 (UTC) 'Support – I also support this, as per nom. Its been a few months and there has been no opposition to this proposal, so I'll probably carry out the merger shortly (probably by merging "Phase contrast microscope" into "Phase contrast microscopy"). A short section on "phase contrast in electron microscopy", with a link to Phase-contrast imaging, should also be added. Peter G Werner (talk) 20:19, 8 January 2008 (UTC)[reply]

how to make a phase contrast microscope in the form of a school level project?

If you've already got a microscope that can do Köhler illumination, "all" you'll need to do is to introduce a mask in the condensor upper focal plane and a phase-shift ring into the back objective focal plane. From a handiwork point of view, the mask should certainly be do-able, as it's "just" a piece of thin metal with a ring-shaped hole cut into it, and then you mount the thing at the right position in the microscope. The phase-shift ring however might be more difficult: you'll need to find a glass plate which has *very* parallel surfaces (ie no thickness differences bigger than 0.00001 mm), and then somehow put a ring-shaped layer of stuff on that's approx. 0.001 mm thick (depending on what stuff you choose). Might be worth while looking for a "quater phase plate" on ebay for that...). In short, what I'd try:

See if you can find a microscope with köhler illumination. If you can't, I suggest you build some type of telescope instead.

See if you can find a quater phase plate that you can somehow build into your objective.

If both the above are true, read up on how a phase contrast microscope works.

Find out where your various focal planes are.

You'll then need to tailor your mask to the size of the quater-phase ring you found.

Start pimping your microscope:-)

When applying points 1through6: Have fun!

Hope this helps. Cheers Michael zh 13:41, 16 July 2007 (UTC)[reply]