Talk:DNA vaccine/Archive 1

Archive 1

Complete rewrite

I've done a complete rewrite of this article. Biochemza 20:22, 21 November 2007 (UTC)

TODO: Add section on Safety

Including: Safety Issues, Integration, Tolerance, Autoimmunity, Anti-DNA antibodies. Biochemza 22:06, 21 November 2007 (UTC)

Introduction/lead

Hi there, the introduction should be part of the summary, this is explained in the guide to authors on layout - Wikipedia:Guide to layout, part of the main Wikipedia:Manual of Style. Hope this helps, Tim Vickers (talk) 22:52, 21 November 2007 (UTC)

Surely this lead paragraph is too long though? Biochemza 15:12, 23 November 2007 (UTC) —Preceding unsigned comment added by Biochemza (talk • contribs)

Yes, it does need to be condensed a little bit, but the lead isn't like the abstract of a paper, more a cross between an abstract and an introduction. Have a look at some of the other scientific featured articles, such as bacteria, DNA or enzyme to get an example of format. Tim Vickers (talk) 18:15, 23 November 2007 (UTC)

Zika

https://www.technologyreview.com/s/602073/us-government-starts-test-of-zika-vaccine-in-humans/

U.S. Government Starts Test of Zika Vaccine in Humans

Scientists are close behind an outbreak of a mosquito-spread disease in the U.S. with a possible fix.

by Antonio Regalado August 2, 2016

The U.S. government on Tuesday for the first time tested an experimental vaccine against the Zika virus on an American volunteer.

The start of the vaccine trial, at the National Institutes of Health in Maryland, comes as officials were racing to contain an outbreak of the Zika virus in Miami, where it has already infected at least 14 people.

A vaccine could roll back the epidemic from U.S. borders, but it won’t be known for months if the government’s vaccine is safe, and it could take years longer to prove it’s effective.

The study involves a novel type of vaccination called a DNA vaccine, in which genes from the virus are shot under high pressure into a person’s arm. While easy to design, no DNA vaccine has ever reached commercialization. — Preceding unsigned comment added by 24.143.68.7 (talk) 09:31, 3 August 2016 (UTC)

Human Applications?

"One DNA vaccine has been approved for human use." Is it possible to provide a reference for this statement in the Introduction? The rest of the article seems to indicate that there any human applications are currently experimental only. 82.173.146.134 (talk) 09:34, 5 February 2017 (UTC)

Vaccination vs immunization move

This article was recently moved from "DNA vaccination" to "DNA immunization". Google search and PubMed return more results for "DNA vaccination", so isn't that a more appropriate title? Velayinosu (talk) 03:09, 2 December 2020 (UTC)

I moved it back.

Genetics4good, regarding this? Moving an article like this one should be the result of a WP:Requested moves discussion. If you want it moved, start a WP:Requested moves discussion. You claimed that "DNA vaccination" is not a term that is commonly used. But the sources in this article indicate otherwise. And there is what Velayinosu stated above. When it comes to rationales for moving this article, you should be considering WP:Common name and WP:MEDTITLE. Flyer22 Frozen (talk) 01:35, 3 December 2020 (UTC)

I disagree it's a better name, just because you get more hits on "DNA vaccination". The thought here is also that "DNA immunization" appears to be a bit bigger in scope rather then just DNA vaccines. I don't get the exact technical difference between a regular gene therapy and a DNA vaccine (is there one?), but I assume it's just differentiated by its use (DNA vaccines are only used as a preventive measure (against not yet existent personal infections by diseases), whereas gene therapies are used only on people already carrying a (genetic) disease. I'm assuming that by increasing to scope by moving it to DNA immunization, you can also include those techniques that are not administered every time to each patient, but you can include heritable measures too (see Human_germline_engineering and also see my comment at the talk page there concerning non-somatic gene therapies (not sure they exist or can even be made, but if they can be made, it's important in this context, see Talk:Human_germline_engineering)

--Genetics4good (talk) 11:39, 3 December 2020 (UTC)

Requested move 3 December 2020

The following is a closed discussion of a requested move. Please do not modify it. Subsequent comments should be made in a new section on the talk page. Editors desiring to contest the closing decision should consider a move review after discussing it on the closer's talk page. No further edits should be made to this discussion.

The result of the move request was: Consensus to move to DNA vaccine instead. No such user (talk) 10:56, 21 December 2020 (UTC)

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DNA vaccination → DNA immunization – See issues mentioned above Genetics4good (talk) 11:41, 3 December 2020 (UTC) —Relisting. BegbertBiggs (talk) 23:19, 11 December 2020 (UTC)

A lot of the article is about DNA vaccines/vaccination (e.g. theory, examples etc.), as are the refs. Shouldn't it be called DNA vaccine? 109.255.90.188 (talk) 14:09, 3 December 2020 (UTC)

Absolutely agree that a better name would be DNA vaccine - would also be in line with RNA vaccine page.--Iztwoz (talk) 22:58, 17 December 2020 (UTC)

I also support moving this article to DNA vaccine per the references and to match up with the RNA vaccine article name. Some1 (talk) 05:48, 18 December 2020 (UTC)

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The discussion above is closed. Please do not modify it. Subsequent comments should be made on the appropriate discussion page. No further edits should be made to this discussion.

Update for COVID 19 ?

Some of the candidate vaccines for COVID19 use DNA e.g. INO-4800. And there's a new(?) delivery technique called electroporation which could be mentioned. As I understand it a small electric pulse opens pores in the cell to let more plasmids in. Is anyone curating? — Preceding unsigned comment added by Shannock9 (talk • contribs) 16:03, 30 August 2020 (UTC)

I think there is a much larger issue with that. I'll open a new section for that... see here --Max schwalbe (talk) 07:14, 17 January 2021 (UTC)

Semi-protected edit request on 23 June 2021

Source Astrazeneca : with hundreds of millions of DNA vaccines now given , it is important to understand the mechanism of action of the AZ.and other DNA vaccines. The chimpanzee adenovirus infects the cell, gets the DNA into the cytoplasm of the cell. The body recognizes the DNA material and places it in the nucleas where all the other encoded DNA are stored and maintained. The Astra Zeneca researchers and investigators state that there is no incorporation of the genetically engineered piece of DNA into the rest of the body’s genome or DNA library. However, we know from studies on viruses including adenoviruses, that some DNA incorporation may occur. They explain the DNA is transcribed to messenger RNA in the nucleus, exists the nucleus, then binds to a ribosome so that the genetic code written in the messenger RNA is followed. The steps in the cytosol with the binding of the ribosome that follows the goes pretty much along the same route that happens in the Pfizer BioNtech and Moderna. The ribosome transcribes the instruction set to make the spike protein. This spike protein then results in the immune response to make the antibodies, activate the T cells, B cells, etc 2001:8003:6D33:7500:57:BAA4:ECF0:3923 (talk) 13:14, 23 June 2021 (UTC)

 Not done: it's not clear what changes you want to be made. Please mention the specific changes in a "change X to Y" format and provide a reliable source if appropriate. ScottishFinnishRadish (talk) 14:07, 23 June 2021 (UTC)

Semi-protected edit request on 21 August 2021

I request to change the following information in the opening paragraph FROM: An Indian company Cadila Healthcare is undertaking trials for Covid-19 vaccine using plasmid DNA technology, which if approved, will become the first DNA vaccine for human use.

TO: An Indian company Cadila Healthcare has undergone trials for it's Covid-19 vaccine ZyCoV-D using plasmid DNA technology, which has been approved to emergency use in India. It is the first DNA vaccine for human use.

SOURCES: https://www.thehindu.com/news/national/zycov-d-indias-first-covid-19-vaccine-for-those-above-12-gets-nod/article36021251.ece https://pib.gov.in/PressReleasePage.aspx?PRID=1747669 mohitraj (talk) 12:22, 21 August 2021 (UTC)

 Partly done: Updated to say that it is approved, no reliable source for it being the first DNA vaccine. Sources only quote some guy from the Cadila Healthcare saying so, not that it is actually the case. It is the first (approved) DNA vaccine against covid. 15 (talk) 20:12, 22 August 2021 (UTC)

Semi-protected edit request on 20 August 2021

Please change the last sentence in the lead paragraph from

• An Indian company Cadila Healthcare is undertaking trials for Covid-19 vaccine using plasmid DNA technology, which if approved, will become the first DNA vaccine for human use.

to

• An Indian company Cadila Healthcare has successfully completed trials for Covid-19 vaccine using plasmid DNA technology, which is now approved for emergency use. Thus, it became the first COVID-19 DNA vaccine for human use.

References

2409:4061:2E0C:3F5:D0A2:B5A:94BF:112F (talk) 17:16, 20 August 2021 (UTC)

 Done Reworded and moved to history, undue for lead. 15 (talk) 20:13, 22 August 2021 (UTC)

Semi-protected edit request on 26 August 2021

Add that Cadilla's DNA vaccine has been approved by India. Source-https://www.indiatoday.in/coronavirus-outbreak/story/zydus-cadila-s-three-dose-covid-vaccine-zycov-d-gets-dcgi-nod-for-eua-1843439-2021-08-20 1.186.77.18 (talk) 02:44, 26 August 2021 (UTC)

 Already done ScottishFinnishRadish (talk) 10:50, 26 August 2021 (UTC)

Semi-protected edit request on 27 August 2021

Please change from

• it is the first DNA vaccine approved for humans.

to

• it is the first COVID 19 DNA vaccine approved for humans.

in the last line of the lead paragraph, because its not the first DNA vaccine but first DNA vaccine for COVID 19. The present statement is partially incorrect. 42.105.102.119 (talk) 10:59, 27 August 2021 (UTC)

 Not done for now: What other DNA vaccines have been approved for use in humans? ScottishFinnishRadish (talk) 11:07, 27 August 2021 (UTC)

Extremely technical discussion.

I predict that 95% of readers coming to this page will find it nearly useless, because nearly every sentence contains undefined technical terms while many sentences contain multiple such terms. Moreover for a naive reader, trying to follow up all the cross-references would be a nearly hopeless task because they also contain many undefined terms. I understand that trying to define each term here would vastly lengthen the article and is impractical. Nevertheless it would help a great deal if a separate, far less technical section was provided that summarized key points in simple language. I also recognize this is a widespread problem in many technical fields such as mathematics and physics. Perhaps someone could develop a standard for providing two levels of discussion in such cases. (Forgive my ignorance if such a standard already exists.) Burressd (talk) 09:00, 23 September 2021 (UTC)

Potential of insertion into genome

The potential of integration in host genomes ist currently not in the article, although it is mentioned by several reviews. The original reports are here:

--Ghilt (talk) 10:53, 6 November 2021 (UTC)