Talk:CSPD (molecule)

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This article was the subject of a Wiki Education Foundation-supported course assignment, between 1 September 2021 and 6 December 2021. Further details are available on the course page. Student editor(s): Abdul.mamun47.

Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 16:30, 16 January 2022 (UTC)[reply]

Proposal to move/merge artice

This article seems too specialized. As far as I can tell, CSPD is just one of many similar reagents used in enhanced-chemiluminescence variants of ELISA assays. The enhanced chemiluminescence article currently is a redirect to chemiluminescence. Methinks that this article and the "Enhanced chemiluminescence" section of chemiluminescence should be merged and either become a section of the ELISA article, or, better, made into a new article, enhanced chemiluminescence ELISA. What do you think? All the best, --Jorge Stolfi (talk) 15:19, 10 July 2009 (UTC)[reply]

I would argue that this article is for a reagent and therefore should remain a separate article. The two topics that it is suggested that this be merged with are about analytical techniques which may (or may not) utilise this reagent. Therefore it seems prudent for the afforementioned articles to mention this compound and provide a link to this page where a reader may discover the structure and the reactivity of the compound. -- The chemistds (talk) 14:32, 2 January 2011 (UTC)[reply]

Wrong equation

This article is named after one substance (CSPD) but the equations refer to another similar but distinct substance (AMPPD). --Jorge Stolfi (talk) 18:34, 10 July 2009 (UTC)[reply]

Incomplete name

The text says that one of the intermetiate products is 1,2-dioxetane. But from the references and the equation it seems to be a *derivative* of 1,2-dioxetane. --Jorge Stolfi (talk) 18:34, 10 July 2009 (UTC)[reply]

Incorrect descriptions

I have removed the following text ... In typical uses of ELISA kits, the enzyme alkaline phosphatase removes the phosphate group from CSPD, yielding a reactive anion (phenolate, which then splits itself in two components, adamantane and 1,2-dioxetane. This second reaction emits turquoise-coloured light (λ_max = 477 nm). The decomposition of the dioxetane generates a secondary glow.^[1]^[2]

AMPPD{\overrightarrow {_{AP}}}AMPD^{-}+P

(A = adamantyl, M = methoxy, P = phenyl , P = phosphate, D = 1,2-dioxetane

The Baeyer energy that is stored in the ring, is emitted as 477 nm light.

AMPD^{-}\rightarrow A+MP+h\nu

...because there are problems with the descriptions of the chemical reactions involved including:

Adamantane and 1,2-dioxetane themselves are not products or intermediates of these reactions, but rather derivatives of these compounds
It is really not clear which reaction is emitting the 477 nm chemiluminescence and which other one "generates a secondary glow". Is light really emitted in two separate steps?
The chemical equation describes the reactions of AMPPD, not CSPD (the subject of this article)

-- Ed (Edgar181) 17:37, 22 June 2011 (UTC)[reply]

References

^ Stephan Beck, Hubert Koster (1990). "Applications of dioxetane chemiluminescent probes to molecular biology". Anal. Chem. 62 (21): 2258–2270. doi:10.1021/ac00220a003.
^ US 5094939, Masahisa Okada, Yoshihiro Ashihara, Tadashi Ninomiya, Akira Yano, "Chemiluminescence assays using stabilized dioxetane derivatives", published 1992

[beck-1] Stephan Beck, Hubert Koster (1990). "Applications of dioxetane chemiluminescent probes to molecular biology". Anal. Chem. 62 (21): 2258–2270. doi:10.1021/ac00220a003.

[patent1-2] US 5094939, Masahisa Okada, Yoshihiro Ashihara, Tadashi Ninomiya, Akira Yano, "Chemiluminescence assays using stabilized dioxetane derivatives", published 1992

[1]

[2]