Talk:Bacterial artificial chromosome

	This article is within the scope of WikiProject Molecular Biology, a collaborative effort to improve the coverage of Molecular Biology on Wikipedia. If you would like to participate, please visit the project page, where you can join the discussion and see a list of open tasks.Molecular BiologyWikipedia:WikiProject Molecular BiologyTemplate:WikiProject Molecular BiologyMolecular Biology articles
???	This article has not yet received a rating on the importance scale.
	This article is supported by the Molecular and Cell Biology task force (assessed as Mid-importance).

Shape/more details...

Are the BACs linear or circular? What are the ends like if they are linear?

Assembler01 19:14, 15 March 2007 (UTC)assembler01[reply]

Circular; They are maintained and amplified within E.coli cells and thus need to be circular. Mistake in the article: According to a paper by Zimmer and Gibbins (1997), Genomics 42, the maximum length for an insert in a BAC is not 350 kbp but probably above 600 kbp. 137.248.179.1 (talk) 13:21, 23 July 2008 (UTC)[reply]

BAC and DNA FISH

BACs are used extensively in biomolecular methods other than just cloning. DNA fluorescence in-situ hybridization (DNA FISH) comes to mind. This article should include this information. —Preceding unsigned comment added by 64.134.66.168 (talk) 00:00, 5 August 2010 (UTC) DGD[reply]

History of BACs

I have here a statement (that is, locally noted down from a lecture I once heard) that BACs were originally based on the F-plasmid. Evidently different constructs can be used for a BAC. The main article should mention the history, e. g. when they were first used, first called that way, and so forth - ideally close to the start of the article. It may also be helpful to summarize the difference between BACs, PACs and YACs, also from a historic point of view. Right now we don't have this in the main articl here. 2A02:8388:1641:8380:0:0:0:2 (talk) 17:25, 5 December 2019 (UTC)[reply]

Storage capacity of BACs

The book "Molekulare Genetik" by Rolf Knippers also mentions a maximum capacity of 350kb. However had, other articles on the world wide web cite a lower maximal capacity. It would be nice to detail more about the capacity, e. g. multiple sources that mention it. 2A02:8388:1643:D680:C23D:D743:2A9C:ACFE (talk) 00:35, 28 July 2024 (UTC)[reply]