Talk:Actin depolymerizing factor

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Hello, I was thinking of adding some more content to this article. Here is the outline of some of the elements I was thinking of adding let me know if this seems appropriate or if I should add or remove anything. Thank you. Outline

A. Actin Depolymerizing factor is a family of proteins involved in the depolymerization of actin polymers

Consist of Cofilins and ADF B. Mechanism of action [4]

2. Cofilins/ADF binds to F-actin subunits and twists them

3. this twisting makes F actin unable to bind phalloidin, which is necessary for structural stability. this destabilizes actin filaments

4. Binding of ADF is cooperative and induces conformational change

5. ATP bound F Actin hydrolyzes ATP to ADP and Pi [5] Picture of the F-actin binding site [3]

a. release of Pi destabilizes actin, cofilin binds released Pi

C. Regulation

Regulated by capped ends of short actin filaments stop actin depolymerizing factors from binding to F sites 2. Competitive binding with Tropomyosin [4]

a. Tropomyosin binds actin uncooperatively (does not induce conformational change)

b. when actin is bound to tropomyosin it cannot bind ADF

3. PH changes [4]

a. Ph increases also increase concentration of ADF present and increases rate of actin depolymerization

b. Alkaline environment promotes weaker Pi binding to actin so Pi is more likely to be release which destabilizes actin [5]

4. Phosphorylation

a. Phosphorylation of Serine leads to deactivation of ADF, dephosphorylation activates ADF — Preceding unsigned comment added by Dwilfand (talk • contribs) 22:07, 10 May 2018 (UTC)[reply]