TRIM25

TRIM25
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4CFG, 4LTB, 5FER
Identifiers
Aliases	TRIM25, EFP, RNF147, Z147, ZNF147, tripartite motif containing 25
External IDs	OMIM: 600453; MGI: 102749; HomoloGene: 48325; GeneCards: TRIM25; OMA:TRIM25 - orthologs
EC number	2.3.2.27
Gene location (Human) Chr. Chromosome 17 (human) Band 17q22 Start 56,836,387 bp End 56,914,080 bp
Gene location (Mouse) Chr. Chromosome 11 (mouse) Band 11|11 C Start 88,890,202 bp End 88,911,119 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in mucosa of ileum blood pancreatic epithelial cell lower lobe of lung jejunal mucosa monocyte appendix thymus skin of hip spleen Top expressed in Paneth cell medullary collecting duct renal corpuscle semi-lunar valve conjunctival fornix retinal pigment epithelium ciliary body endothelial cell of lymphatic vessel ureter epithelium of stomach More reference expression data BioGPS More reference expression data
Gene ontology Molecular function transferase activity DNA-binding transcription factor activity ubiquitin protein ligase activity metal ion binding ligase activity protein binding ubiquitin-protein transferase activity RNA binding cadherin binding zinc ion binding Cellular component nucleoplasm cytoplasm cytosol nuclear body intracellular anatomical structure Biological process ERAD pathway negative regulation of viral entry into host cell immune system process interferon-gamma-mediated signaling pathway positive regulation of DNA-binding transcription factor activity protein monoubiquitination defense response to virus positive regulation of NF-kappaB transcription factor activity protein ubiquitination positive regulation of I-kappaB kinase/NF-kappaB signaling translesion synthesis negative regulation of type I interferon production innate immune response viral process response to estrogen response to vitamin D protein deubiquitination ubiquitin-dependent ERAD pathway cellular response to leukemia inhibitory factor ubiquitin-dependent protein catabolic process regulation of viral entry into host cell Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 7706 217069 Ensembl ENSG00000121060 ENSMUSG00000000275 UniProt Q14258 Q61510 RefSeq (mRNA) NM_005082 NM_009546 RefSeq (protein) NP_005073 NP_033572 Location (UCSC) Chr 17: 56.84 – 56.91 Mb Chr 11: 88.89 – 88.91 Mb PubMed search
Wikidata
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Tripartite motif-containing protein 25 is a protein that in humans is encoded by the TRIM25 gene.

Function

The protein encoded by this gene is a member of the tripartite motif (TRIM) family grouping more than 70 TRIMs. TRIM proteins primarily function as ubiquitin ligases that regulate the innate response to infection. TRIM25 localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.

Domain Architecture

TRIM25 has an N-terminal RING domain, followed by a B-box type 1 domain, a B-box type 2 domain, a coiled-coil domain (CCD) and a C-terminal SPRY domain. The RING domain coordinates two zinc atoms and is essential for recruiting ubiquitin-conjugating enzymes. The function of the B-box domains is unknown. The CCD domain has been implicated in multimerization and other protein-protein interactions. The SPRY domain is required for substrate recruitment. The NMR chemical shifts for backbone of the PRYSPRY domain of TRIM25 is assigned based on triple-resonance experiments using uniformly isotopic labeled protein and the secondary structure of the domain PRYSPRY domain of TRIM25 predicted based on the NMR assignments.

TRIM25 functions

TRIM25 plays a key role in the RIG-I signaling pathway. RIG-I is a cytosolic pattern recognition receptor that senses viral RNA. Following RNA recognition, the caspase recruitment domain (CARD) of RIG-I undergoes K(63)-linked ubiquitination by TRIM25. The RING and SPRY domains of TRIM25 mediate its interaction with RIG-I. IFN production then follows by an intracellular signaling pathway involving IRF3.

Viral escape

To avoid IFN production, the non structural protein (NS1) of influenza will interact with CCD domain of TRIM25 to block RIG-I ubiquitination. Some studies have shown that a deletion of the CCD domain of TRIM25 prevents the binding of NS1. Without this ubiquitination, there won’t be IFN production.

Further reading

• Horie K, Urano T, Ikeda K, Inoue S (Jun 2003). "Estrogen-responsive RING finger protein controls breast cancer growth". The Journal of Steroid Biochemistry and Molecular Biology. 85 (2–5): 101–4. doi:10.1016/S0960-0760(03)00209-7. PMID 12943693. S2CID 22487508.
• Inoue S, Orimo A, Hosoi T, Kondo S, Toyoshima H, Kondo T, Ikegami A, Ouchi Y, Orimo H, Muramatsu M (Dec 1993). "Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein". Proceedings of the National Academy of Sciences of the United States of America. 90 (23): 11117–21. doi:10.1073/pnas.90.23.11117. PMC 47933. PMID 8248217.
• Bonaldo MF, Lennon G, Soares MB (Sep 1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Research. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Ikeda K, Inoue S, Orimo A, Sano M, Watanabe T, Tsutsumi K, Muramatsu M (Jul 1997). "Multiple regulatory elements and binding proteins of the 5'-flanking region of the human estrogen-responsive finger protein (efp) gene". Biochemical and Biophysical Research Communications. 236 (3): 765–71. doi:10.1006/bbrc.1997.7046. PMID 9245730.
• Ikeda K, Orimo A, Higashi Y, Muramatsu M, Inoue S (Apr 2000). "Efp as a primary estrogen-responsive gene in human breast cancer". FEBS Letters. 472 (1): 9–13. doi:10.1016/S0014-5793(00)01421-6. PMID 10781795. S2CID 10570937.
• Reymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L, Riganelli D, Zanaria E, Messali S, Cainarca S, Guffanti A, Minucci S, Pelicci PG, Ballabio A (May 2001). "The tripartite motif family identifies cell compartments". The EMBO Journal. 20 (9): 2140–51. doi:10.1093/emboj/20.9.2140. PMC 125245. PMID 11331580.
• Urano T, Saito T, Tsukui T, Fujita M, Hosoi T, Muramatsu M, Ouchi Y, Inoue S (Jun 2002). "Efp targets 14-3-3 sigma for proteolysis and promotes breast tumour growth". Nature. 417 (6891): 871–5. Bibcode:2002Natur.417..871U. doi:10.1038/nature00826. PMID 12075357. S2CID 4348545.
• Shimada N, Suzuki T, Inoue S, Kato K, Imatani A, Sekine H, Ohara S, Shimosegawa T, Sasano H (Apr 2004). "Systemic distribution of estrogen-responsive finger protein (Efp) in human tissues". Molecular and Cellular Endocrinology. 218 (1–2): 147–53. doi:10.1016/j.mce.2003.12.008. PMID 15130519. S2CID 44761828.
• Rush J, Moritz A, Lee KA, Guo A, Goss VL, Spek EJ, Zhang H, Zha XM, Polakiewicz RD, Comb MJ (Jan 2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nature Biotechnology. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455. S2CID 7200157.
• Suzuki T, Urano T, Tsukui T, Horie-Inoue K, Moriya T, Ishida T, Muramatsu M, Ouchi Y, Sasano H, Inoue S (Sep 2005). "Estrogen-responsive finger protein as a new potential biomarker for breast cancer". Clinical Cancer Research. 11 (17): 6148–54. doi:10.1158/1078-0432.CCR-05-0040. PMID 16144914. S2CID 11698115.
• Nakayama H, Sano T, Motegi A, Oyama T, Nakajima T (Nov 2005). "Increasing 14-3-3 sigma expression with declining estrogen receptor alpha and estrogen-responsive finger protein expression defines malignant progression of endometrial carcinoma". Pathology International. 55 (11): 707–15. doi:10.1111/j.1440-1827.2005.01900.x. PMID 16271083. S2CID 7106422.
• Nakasato N, Ikeda K, Urano T, Horie-Inoue K, Takeda S, Inoue S (Dec 2006). "A ubiquitin E3 ligase Efp is up-regulated by interferons and conjugated with ISG15". Biochemical and Biophysical Research Communications. 351 (2): 540–6. doi:10.1016/j.bbrc.2006.10.061. PMID 17069755.
• Nakajima A, Maruyama S, Bohgaki M, Miyajima N, Tsukiyama T, Sakuragi N, Hatakeyama S (May 2007). "Ligand-dependent transcription of estrogen receptor alpha is mediated by the ubiquitin ligase EFP". Biochemical and Biophysical Research Communications. 357 (1): 245–51. doi:10.1016/j.bbrc.2007.03.134. hdl:2115/24261. PMID 17418098.