Split TEV
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The split TEV technique[1] is a molecular method to monitor protein-protein interactions in living cells. It is based on the functional reconstitution of two previously inactive fragments derived from the NIa protease of the tobacco etch virus (TEV protease). These fragments, either an N-terminal (NTEV) or C-terminal part (CTEV), are fused to protein interaction partners of choice. Upon interaction of the two candidate proteins, the NTEV and CTEV fragments get into close proximity, regain proteolytic activity, and activate specific TEV reporters which indicate an occurred protein-protein interaction.
References
[edit]- ^ Wehr, Michael C; Laage, Rico; Bolz, Ulrike; Fischer, Tobias M; Grünewald, Sylvia; Scheek, Sigrid; Bach, Alfred; Nave, Klaus-Armin; Rossner, Moritz J (2006). "Monitoring regulated protein-protein interactions using split TEV". Nature Methods. 3 (12): 985–93. doi:10.1038/nmeth967. PMID 17072307.
Further reading
[edit]- Wehr, Michael C; Reinecke, Lisa; Botvinnik, Anna; Rossner, Moritz J (2008). "Analysis of transient phosphorylation-dependent protein-protein interactions in living mammalian cells using split-TEV". BMC Biotechnology. 8: 55. doi:10.1186/1472-6750-8-55. PMC 2483975. PMID 18620601.
- Djannatian, Minou S.; Galinski, Sabrina; Fischer, Tobias M.; Rossner, Moritz J. (2011). "Studying G protein-coupled receptor activation using split-tobacco etch virus assays". Analytical Biochemistry. 412 (2): 141–52. doi:10.1016/j.ab.2011.01.042. PMID 21295005.