SEPT9

SEPTIN9
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	4YQF
Identifiers
Aliases	SEPTIN9, AF17q25, MSF, MSF1, NAPB, PNUTL4, SINT1, SeptD1, septin 9, SEPT9
External IDs	OMIM: 604061; MGI: 1858222; HomoloGene: 90949; GeneCards: SEPTIN9; OMA:SEPTIN9 - orthologs
Gene location (Human)
Chr.	Chromosome 17 (human)
End	77,500,596 bp
Gene location (Mouse)
Chr.	Chromosome 11 (mouse)
End	117,253,151 bp
RNA expression pattern
	Top expressed in
	mucosa of ileum; ; granulocyte; ; thymus; ; parotid gland; ; ventricular zone; ; lymph node; ; stromal cell of endometrium; ; appendix; ; ganglionic eminence; ; pancreatic ductal cell;
	Top expressed in
	tail of embryo; ; granulocyte; ; ventricular zone; ; genital tubercle; ; ascending aorta; ; thymus; ; choroid plexus of fourth ventricle; ; internal carotid artery; ; calvaria; ; mandibular prominence;
	More reference expression data
	n/a
Gene ontology
Molecular function	nucleotide binding; GTP binding; protein binding; GTPase activity; cadherin binding; molecular adaptor activity;
Cellular component	perinuclear region of cytoplasm; axoneme; septin complex; microtubule; cytoskeleton; stress fiber; actin cytoskeleton; cytoplasm; non-motile cilium; septin ring; microtubule cytoskeleton;
Biological process	cell division; cell cycle; positive regulation of non-motile cilium assembly; protein heterooligomerization; cilium assembly; cytoskeleton-dependent cytokinesis;
	Sources:Amigo / QuickGO
Orthologs
	10801
	53860
	ENSG00000282302; ENSG00000184640
	ENSMUSG00000059248
	Q9UHD8
	Q80UG5
NM_001113491; NM_001113492; NM_001113493; NM_001113494; NM_001113495;
	NM_001113496; NM_001293695; NM_001293696; NM_001293697; NM_001293698; NM_006640
	NM_001113486; NM_001113487; NM_001113488; NM_017380
NP_001106963; NP_001106964; NP_001106965; NP_001106966; NP_001106967;
	NP_001106968; NP_001280624; NP_001280625; NP_001280626; NP_001280627; NP_006631
	NP_001106958; NP_001106959; NP_001106960; NP_059076
	Wikidata
View/Edit Human	View/Edit Mouse

Septin-9 is a protein that in humans is encoded by the SEPT9 gene.^[5]^[6]^[7]

Interactions

SEPT9 has been shown to interact with SEPT2^[8] and SEPT7.^[8]

Function

Along with AHNAK, eIF4E and S100A11, SEPT9 has been shown to be essential for pseudopod protrusion, tumor cell migration and invasion.^[9]

Clinical significance

The v2 region of the SEPT9 promoter has been shown to be methylated in colorectal cancer tissue compared with normal colonic mucosa.^[10] Using highly sensitive real time PCR assays, methylated SEPT9 was detected in the blood of colorectal cancer patients. This alternate methylation pattern in cancer samples is suggestive of an aberrant activation or repression of the gene compared to normal tissue samples.^[11]^[12]

Testing to detect methylated SEPT9 is not indicated as a first option for colorectal cancer screening.^[13] It is similar in specificity and sensitivity to the stool guaiac test or fecal immune tests, and those tests should be used in preference.^[13] In cases when the physician aggressively has recommended a colonoscopy and the patient has declined that and these other tests, then this test has advantages over patients having no screening at all.^[13]

References

^ ^a ^b ^c ENSG00000184640 GRCh38: Ensembl release 89: ENSG00000282302, ENSG00000184640 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000059248 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Osaka M, Rowley JD, Zeleznik-Le NJ (May 1999). "MSF (MLL septin-like fusion), a fusion partner gene of MLL, in a therapy-related acute myeloid leukemia with a t(11;17)(q23;q25)". Proceedings of the National Academy of Sciences of the United States of America. 96 (11): 6428–6433. Bibcode:1999PNAS...96.6428O. doi:10.1073/pnas.96.11.6428. PMC 26898. PMID 10339604.
^ Taki T, Ohnishi H, Shinohara K, Sako M, Bessho F, Yanagisawa M, Hayashi Y (September 1999). "AF17q25, a putative septin family gene, fuses the MLL gene in acute myeloid leukemia with t(11;17)(q23;q25)". Cancer Research. 59 (17): 4261–4265. PMID 10485469.
^ "Entrez Gene: SEPT9 septin 9".
^ ^a ^b Surka MC, Tsang CW, Trimble WS (October 2002). "The mammalian septin MSF localizes with microtubules and is required for completion of cytokinesis". Molecular Biology of the Cell. 13 (10): 3532–3545. doi:10.1091/mbc.E02-01-0042. PMC 129964. PMID 12388755.
^ Shankar J, Messenberg A, Chan J, Underhill TM, Foster LJ, Nabi IR (May 2010). "Pseudopodial actin dynamics control epithelial-mesenchymal transition in metastatic cancer cells". Cancer Research. 70 (9): 3780–3790. doi:10.1158/0008-5472.CAN-09-4439. PMID 20388789.
^ Church TR, Wandell M, Lofton-Day C, Mongin SJ, Burger M, Payne SR, et al. (February 2014). "Prospective evaluation of methylated SEPT9 in plasma for detection of asymptomatic colorectal cancer". Gut. 63 (2): 317–325. doi:10.1136/gutjnl-2012-304149. PMC 3913123. PMID 23408352.
^ Grützmann R, Molnar B, Pilarsky C, Habermann JK, Schlag PM, Saeger HD, et al. (2008). "Sensitive detection of colorectal cancer in peripheral blood by septin 9 DNA methylation assay". PLOS ONE. 3 (11): e3759. Bibcode:2008PLoSO...3.3759G. doi:10.1371/journal.pone.0003759. PMC 2582436. PMID 19018278.
^ deVos T, Tetzner R, Model F, Weiss G, Schuster M, Distler J, et al. (July 2009). "Circulating methylated SEPT9 DNA in plasma is a biomarker for colorectal cancer". Clinical Chemistry. 55 (7): 1337–1346. doi:10.1373/clinchem.2008.115808. PMID 19406918.
^ ^a ^b ^c
American Society for Clinical Pathology, "Five Things Physicians and Patients Should Question", Choosing Wisely: an initiative of the ABIM Foundation, American Society for Clinical Pathology, retrieved August 1, 2013, which cites
- Thompson AJ, Muir AJ, Sulkowski MS, Ge D, Fellay J, Shianna KV, et al. (July 2010). "Interleukin-28B polymorphism improves viral kinetics and is the strongest pretreatment predictor of sustained virologic response in genotype 1 hepatitis C virus". Gastroenterology. 139 (1): 120–9.e18. doi:10.1053/j.gastro.2010.04.013. PMID 20399780.
- Ahlquist DA, Taylor WR, Mahoney DW, Zou H, Domanico M, Thibodeau SN, et al. (March 2012). "The stool DNA test is more accurate than the plasma septin 9 test in detecting colorectal neoplasia". Clinical Gastroenterology and Hepatology. 10 (3): 272–7.e1. doi:10.1016/j.cgh.2011.10.008. PMC 3980432. PMID 22019796.

Pellegrino JE, George RA, Biegel J, Farlow MR, Gardner K, Caress J, et al. (December 1997). "Hereditary neuralgic amyotrophy: evidence for genetic homogeneity and mapping to chromosome 17q25". Human Genetics. 101 (3): 277–283. doi:10.1007/s004390050629. PMID 9439655. S2CID 20263885.
Nagase T, Ishikawa K, Suyama M, Kikuno R, Hirosawa M, Miyajima N, et al. (February 1999). "Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research. 6 (1): 63–70. doi:10.1093/dnares/6.1.63. PMID 10231032.
Medina M, Marinescu RC, Overhauser J, Kosik KS (January 2000). "Hemizygosity of delta-catenin (CTNND2) is associated with severe mental retardation in cri-du-chat syndrome". Genomics. 63 (2): 157–164. doi:10.1006/geno.1999.6090. PMID 10673328.
Kalikin LM, Sims HL, Petty EM (January 2000). "Genomic and expression analyses of alternatively spliced transcripts of the MLL septin-like fusion gene (MSF) that map to a 17q25 region of loss in breast and ovarian tumors". Genomics. 63 (2): 165–172. doi:10.1006/geno.1999.6077. PMID 10673329.
Russell SE, McIlhatton MA, Burrows JF, Donaghy PG, Chanduloy S, Petty EM, et al. (September 2000). "Isolation and mapping of a human septin gene to a region on chromosome 17q, commonly deleted in sporadic epithelial ovarian tumors". Cancer Research. 60 (17): 4729–4734. PMID 10987277.
McIlhatton MA, Burrows JF, Donaghy PG, Chanduloy S, Johnston PG, Russell SE (September 2001). "Genomic organization, complex splicing pattern and expression of a human septin gene on chromosome 17q25.3". Oncogene. 20 (41): 5930–5939. doi:10.1038/sj.onc.1204752. PMID 11593400.
Yamamoto K, Shibata F, Yamaguchi M, Miura O (June 2002). "Fusion of MLL and MSF in adult de novo acute myelomonocytic leukemia (M4) with t(11;17)(q23;q25)". International Journal of Hematology. 75 (5): 503–507. doi:10.1007/BF02982114. PMID 12095151. S2CID 28022344.
Surka MC, Tsang CW, Trimble WS (October 2002). "The mammalian septin MSF localizes with microtubules and is required for completion of cytokinesis". Molecular Biology of the Cell. 13 (10): 3532–3545. doi:10.1091/mbc.E02-01-0042. PMC 129964. PMID 12388755.
Nagata K, Kawajiri A, Matsui S, Takagishi M, Shiromizu T, Saitoh N, et al. (May 2003). "Filament formation of MSF-A, a mammalian septin, in human mammary epithelial cells depends on interactions with microtubules". The Journal of Biological Chemistry. 278 (20): 18538–18543. doi:10.1074/jbc.M205246200. PMID 12626509.
Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, et al. (August 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America. 101 (33): 12130–12135. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
Nagata K, Asano T, Nozawa Y, Inagaki M (December 2004). "Biochemical and cell biological analyses of a mammalian septin complex, Sept7/9b/11". The Journal of Biological Chemistry. 279 (53): 55895–55904. doi:10.1074/jbc.M406153200. PMID 15485874.
Scott M, Hyland PL, McGregor G, Hillan KJ, Russell SE, Hall PA (July 2005). "Multimodality expression profiling shows SEPT9 to be overexpressed in a wide range of human tumours". Oncogene. 24 (29): 4688–4700. doi:10.1038/sj.onc.1208574. PMID 15782116.
Ito H, Iwamoto I, Morishita R, Nozawa Y, Narumiya S, Asano T, Nagata K (October 2005). "Possible role of Rho/Rhotekin signaling in mammalian septin organization". Oncogene. 24 (47): 7064–7072. doi:10.1038/sj.onc.1208862. PMID 16007136.
Scott M, McCluggage WG, Hillan KJ, Hall PA, Russell SE (March 2006). "Altered patterns of transcription of the septin gene, SEPT9, in ovarian tumorigenesis". International Journal of Cancer. 118 (5): 1325–1329. doi:10.1002/ijc.21486. PMID 16161048. S2CID 37031210.
Kuhlenbäumer G, Hannibal MC, Nelis E, Schirmacher A, Verpoorten N, Meuleman J, et al. (October 2005). "Mutations in SEPT9 cause hereditary neuralgic amyotrophy". Nature Genetics. 37 (10): 1044–1046. doi:10.1038/ng1649. PMID 16186812. S2CID 1756558.

[refGRCh38Ensembl-1] ENSG00000184640 GRCh38: Ensembl release 89: ENSG00000282302, ENSG00000184640 – Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000059248 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid10339604-5] Osaka M, Rowley JD, Zeleznik-Le NJ (May 1999). "MSF (MLL septin-like fusion), a fusion partner gene of MLL, in a therapy-related acute myeloid leukemia with a t(11;17)(q23;q25)". Proceedings of the National Academy of Sciences of the United States of America. 96 (11): 6428–6433. Bibcode:1999PNAS...96.6428O. doi:10.1073/pnas.96.11.6428. PMC 26898. PMID 10339604.

[pmid10485469-6] Taki T, Ohnishi H, Shinohara K, Sako M, Bessho F, Yanagisawa M, Hayashi Y (September 1999). "AF17q25, a putative septin family gene, fuses the MLL gene in acute myeloid leukemia with t(11;17)(q23;q25)". Cancer Research. 59 (17): 4261–4265. PMID 10485469.

[entrez-7] "Entrez Gene: SEPT9 septin 9".

[pmid12388755-8] Surka MC, Tsang CW, Trimble WS (October 2002). "The mammalian septin MSF localizes with microtubules and is required for completion of cytokinesis". Molecular Biology of the Cell. 13 (10): 3532–3545. doi:10.1091/mbc.E02-01-0042. PMC 129964. PMID 12388755.

[pmid20388789-9] Shankar J, Messenberg A, Chan J, Underhill TM, Foster LJ, Nabi IR (May 2010). "Pseudopodial actin dynamics control epithelial-mesenchymal transition in metastatic cancer cells". Cancer Research. 70 (9): 3780–3790. doi:10.1158/0008-5472.CAN-09-4439. PMID 20388789.

[pmid23408352-10] Church TR, Wandell M, Lofton-Day C, Mongin SJ, Burger M, Payne SR, et al. (February 2014). "Prospective evaluation of methylated SEPT9 in plasma for detection of asymptomatic colorectal cancer". Gut. 63 (2): 317–325. doi:10.1136/gutjnl-2012-304149. PMC 3913123. PMID 23408352.

[pmid19018278-11] Grützmann R, Molnar B, Pilarsky C, Habermann JK, Schlag PM, Saeger HD, et al. (2008). "Sensitive detection of colorectal cancer in peripheral blood by septin 9 DNA methylation assay". PLOS ONE. 3 (11): e3759. Bibcode:2008PLoSO...3.3759G. doi:10.1371/journal.pone.0003759. PMC 2582436. PMID 19018278.

[pmid19406918-12] Vos T, Tetzner R, Model F, Weiss G, Schuster M, Distler J, et al. (July 2009). "Circulating methylated SEPT9 DNA in plasma is a biomarker for colorectal cancer". Clinical Chemistry. 55 (7): 1337–1346. doi:10.1373/clinchem.2008.115808. PMID 19406918.

[ASCPfive-13] American Society for Clinical Pathology, "Five Things Physicians and Patients Should Question", Choosing Wisely: an initiative of the ABIM Foundation, American Society for Clinical Pathology, retrieved August 1, 2013, which cites
Thompson AJ, Muir AJ, Sulkowski MS, Ge D, Fellay J, Shianna KV, et al. (July 2010). "Interleukin-28B polymorphism improves viral kinetics and is the strongest pretreatment predictor of sustained virologic response in genotype 1 hepatitis C virus". Gastroenterology. 139 (1): 120–9.e18. doi:10.1053/j.gastro.2010.04.013. PMID 20399780.

Ahlquist DA, Taylor WR, Mahoney DW, Zou H, Domanico M, Thibodeau SN, et al. (March 2012). "The stool DNA test is more accurate than the plasma septin 9 test in detecting colorectal neoplasia". Clinical Gastroenterology and Hepatology. 10 (3): 272–7.e1. doi:10.1016/j.cgh.2011.10.008. PMC 3980432. PMID 22019796.

[14] Thompson AJ, Muir AJ, Sulkowski MS, Ge D, Fellay J, Shianna KV, et al. (July 2010). "Interleukin-28B polymorphism improves viral kinetics and is the strongest pretreatment predictor of sustained virologic response in genotype 1 hepatitis C virus". Gastroenterology. 139 (1): 120–9.e18. doi:10.1053/j.gastro.2010.04.013. PMID 20399780.

[15] Ahlquist DA, Taylor WR, Mahoney DW, Zou H, Domanico M, Thibodeau SN, et al. (March 2012). "The stool DNA test is more accurate than the plasma septin 9 test in detecting colorectal neoplasia". Clinical Gastroenterology and Hepatology. 10 (3): 272–7.e1. doi:10.1016/j.cgh.2011.10.008. PMC 3980432. PMID 22019796.

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SEPT9

Interactions

Function

Clinical significance

See also

References

Further reading