SAND DNA-binding protein domain
| SAND | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 Solution structure of the SAND domain of the putative nuclear protein homolog (5830484a20rik) | |||||||||
| Identifiers | |||||||||
| Symbol | SAND | ||||||||
| Pfam | PF01342 | ||||||||
| InterPro | IPR000770 | ||||||||
| SCOP2 | 1h5p / SCOPe / SUPFAM | ||||||||
| |||||||||
In molecular biology, the protein domain SAND is named after a range of proteins in the protein family: Sp100, AIRE-1, NucP41/75, DEAF-1. It is localised in the cell nucleus and has an important function in chromatin-dependent transcriptional control. It is found solely in eukaryotes.
Function
[edit]The precise function of the protein domain SAND remains to be determined. Nevertheless, it is thought to be a DNA binding domain despite its beta structure. This function can be inferred by studying the DEAF-1 transcription factor.[1] Here, the conserved positively charged residues in the SAND domains suggest the existence of negatively charged ligands. DNA is a negatively charged molecule due to the phosphate found in its backbone. Henceforth, this suggests that the SAND domain is the DNA-binding region of DEAF-1.[2]
Structure
[edit]The structure of this protein domain contains a globular fold. It is thought to have an alpha/beta secondary structure that consists of five beta strands.[2] This structure is made up of a five-stranded antiparallel beta-sheet with four alpha-helices. Further, the SAND domain is thought to have a modular structure; it can be associated with the bromodomain, the PHD finger and the MYND finger.[2]
Conservation
[edit]This protein domain has a conserved region of around 80 residues. Mutations in this region lead to various human diseases, particularly in these proteins: Sp100 (Speckled protein 100 kDa), NUDR (Nuclear DEAF-1 related), GMEB (Glucocorticoid Modulatory Element Binding) proteins and AIRE-1 (Autoimmune regulator 1) proteins.[2][3]
Some proteins with SAND domain
[edit]References
[edit]- ^ Wojciak JM, Clubb RT (2001). "Finding the function buried in SAND". Nat Struct Biol. 8 (7): 568–70. doi:10.1038/89582. PMID 11427878. S2CID 32113775.
- ^ a b c d Bottomley MJ, Collard MW, Huggenvik JI, Liu Z, Gibson TJ, Sattler M (2001). "The SAND domain structure defines a novel DNA-binding fold in transcriptional regulation". Nat Struct Biol. 8 (7): 626–33. doi:10.1038/89675. PMID 11427895. S2CID 6642673.
- ^ Gibson TJ, Ramu C, Gemünd C, Aasland R (July 1998). "The APECED polyglandular autoimmune syndrome protein, AIRE-1, contains the SAND domain and is probably a transcription factor". Trends Biochem. Sci. 23 (7): 242–4. doi:10.1016/s0968-0004(98)01231-6. PMID 9697411.