Rhizobium leguminosarum exopolysaccharide glucosyl ketal-pyruvate-transferase
| Rhizobium leguminosarum exopolysaccharide glucosyl ketal-pyruvate-transferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.5.1.98 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Rhizobium leguminosarum exopolysaccharide glucosyl ketal-pyruvate-transferase (EC 2.5.1.98, PssM) is an enzyme with systematic name phosphoenolpyruvate:(D-GlcA-beta-(1->4)-2-O-Ac-D-GlcA-beta-(1->4)-D-Glc-beta-(1->4)-(3-O-CH3-CH2CH(OH)C(O)-D-Gal-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->6))-2(or3)-O-Ac-D-Glc-alpha-(1->6))n 4,6-O-(1-carboxyethan-1,1-diyl)transferase .[1] This enzyme catalyses the following chemical reaction
- phosphoenolpyruvate + [D-GlcA-beta-(1->4)-2-O-Ac-D-GlcA-beta-(1->4)-D-Glc-beta-(1->4)-[3-O-CH3-CH2CH(OH)C(O)-D-Gal-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->6)]-2(or3)-O-Ac-D-Glc-alpha-(1->6)]n [D-GlcA-beta-(1->4)-2-O-Ac-D-GlcA-beta-(1->4)-D-Glc-beta-(1->4)-[3-O-CH3-CH2CH(OH)C(O)-D-Gal-beta-(1->3)-4,6-CH3(COO-)C-D-Glc-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->6)]-2(or3)-O-Ac-D-Glc-alpha-(1->6)]n + phosphate
The enzyme is responsible for pyruvylation of subterminal glucose in the acidic octasaccharide repeating unit of the exopolysaccharide of Rhizobium leguminosarum.
References
[edit]- ^ Ivashina TV, Fedorova EE, Ashina NP, Kalinchuk NA, Druzhinina TN, Shashkov AS, Shibaev VN, Ksenzenko VN (August 2010). "Mutation in the pssM gene encoding ketal pyruvate transferase leads to disruption of Rhizobium leguminosarum bv. viciae-Pisum sativum symbiosis". Journal of Applied Microbiology. 109 (2): 731–42. doi:10.1111/j.1365-2672.2010.04702.x. PMID 20233262.
External links
[edit]- Rhizobium+leguminosarum+exopolysaccharide+glucosyl+ketal-pyruvate-transferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
