RNAIII
RNAIII is a stable 514 nt regulatory RNA transcribed by the P3 promoter of the Staphylococcus aureus quorum-sensing agr system [1][2]). It is the major effector of the agr regulon, which controls the expression of many S. aureus genes encoding exoproteins and cell wall associated proteins plus others encoding regulatory proteins [3][4] The RNAIII transcript also encodes the 26 amino acid δ-haemolysin (Staphylococcus aureus delta toxin) peptide (Hld).[5] RNAIII contains many stem loops, most of which match the Shine-Dalgarno sequence involved in translation initiation of the regulated genes.[5] Some of these interactions are inhibitory, others stimulatory; among the former is the regulatory protein Rot.[6][7] In vitro, RNAIII is expressed post exponentially, inhibiting translation of the surface proteins, notably protein A, while stimulating that of the exoproteins, many of which are tissue-degrading enzymes or cytolysins.[8] Among the latter is the important virulence factor, α-hemolysin (or alpha toxin) (Hla), whose translation RNAIII activates by preventing the formation of an inhibitory foldback loop in the hla mRNA leader.[9]
References
[edit]- ^ Novick RP, Ross HF, Projan SJ, Kornblum J, Kreiswirth B, Moghazeh S (October 1993). "Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule". The EMBO Journal. 12 (10): 3967–75. doi:10.1002/j.1460-2075.1993.tb06074.x. PMC 413679. PMID 7691599.
- ^ Waters LS, Storz G (February 2009). "Regulatory RNAs in bacteria". Cell. 136 (4): 615–28. doi:10.1016/j.cell.2009.01.043. PMC 3132550. PMID 19239884.
- ^ Janzon L, Arvidson S (May 1990). "The role of the delta-lysin gene (hld) in the regulation of virulence genes by the accessory gene regulator (agr) in Staphylococcus aureus". The EMBO Journal. 9 (5): 1391–9. doi:10.1002/j.1460-2075.1990.tb08254.x. PMC 551825. PMID 2328718.
- ^ Novick RP (June 2003). "Autoinduction and signal transduction in the regulation of staphylococcal virulence". Molecular Microbiology. 48 (6): 1429–49. doi:10.1046/j.1365-2958.2003.03526.x. PMID 12791129. S2CID 6847208.
- ^ a b Benito Y, Kolb FA, Romby P, Lina G, Etienne J, Vandenesch F (May 2000). "Probing the structure of RNAIII, the Staphylococcus aureus agr regulatory RNA, and identification of the RNA domain involved in repression of protein A expression". RNA. 6 (5): 668–79. doi:10.1017/S1355838200992550. PMC 1369947. PMID 10836788.
- ^ Geisinger E, Adhikari RP, Jin R, Ross HF, Novick RP (August 2006). "Inhibition of rot translation by RNAIII, a key feature of agr function". Molecular Microbiology. 61 (4): 1038–48. doi:10.1111/j.1365-2958.2006.05292.x. PMID 16879652. S2CID 229424.
- ^ Boisset S, Geissmann T, Huntzinger E, Fechter P, Bendridi N, Possedko M, et al. (June 2007). "Staphylococcus aureus RNAIII coordinately represses the synthesis of virulence factors and the transcription regulator Rot by an antisense mechanism". Genes & Development. 21 (11): 1353–66. doi:10.1101/gad.423507. PMC 1877748. PMID 17545468.
- ^ Dunman PM, Murphy E, Haney S, Palacios D, Tucker-Kellogg G, Wu S, et al. (December 2001). "Transcription profiling-based identification of Staphylococcus aureus genes regulated by the agr and/or sarA loci". Journal of Bacteriology. 183 (24): 7341–53. doi:10.1128/JB.183.24.7341-7353.2001. PMC 95583. PMID 11717293.
- ^ Morfeldt E, Taylor D, von Gabain A, Arvidson S (September 1995). "Activation of alpha-toxin translation in Staphylococcus aureus by the trans-encoded antisense RNA, RNAIII". The EMBO Journal. 14 (18): 4569–77. doi:10.1002/j.1460-2075.1995.tb00136.x. PMC 394549. PMID 7556100.
Further reading
[edit]- Chevalier C, Boisset S, Romilly C, Masquida B, Fechter P, Geissmann T, Vandenesch F, Romby P (March 2010). "Staphylococcus aureus RNAIII binds to two distant regions of coa mRNA to arrest translation and promote mRNA degradation". PLOS Pathogens. 6 (3): e1000809. doi:10.1371/journal.ppat.1000809. PMC 2837412. PMID 20300607.
- Coelho LR, Souza RR, Ferreira FA, Guimarães MA, Ferreira-Carvalho BT, Figueiredo AM (November 2008). "agr RNAIII divergently regulates glucose-induced biofilm formation in clinical isolates of Staphylococcus aureus". Microbiology. 154 (Pt 11). Reading, England: 3480–3490. doi:10.1099/mic.0.2007/016014-0. PMID 18957601.
- Queck SY, Jameson-Lee M, Villaruz AE, Bach TH, Khan BA, Sturdevant DE, Ricklefs SM, Li M, Otto M (October 2008). "RNAIII-independent target gene control by the agr quorum-sensing system: insight into the evolution of virulence regulation in Staphylococcus aureus". Molecular Cell. 32 (1): 150–8. doi:10.1016/j.molcel.2008.08.005. PMC 2575650. PMID 18851841.
- Huntzinger E, Boisset S, Saveanu C, Benito Y, Geissmann T, Namane A, Lina G, Etienne J, Ehresmann B, Ehresmann C, Jacquier A, Vandenesch F, Romby P (February 2005). "Staphylococcus aureus RNAIII and the endoribonuclease III coordinately regulate spa gene expression". The EMBO Journal. 24 (4): 824–35. doi:10.1038/sj.emboj.7600572. PMC 549626. PMID 15678100.
- Chabelskaya S, Bordeau V, Felden B (April 2014). "Dual RNA regulatory control of a Staphylococcus aureus virulence factor". Nucleic Acids Research. 42 (8): 4847–58. doi:10.1093/nar/gku119. PMC 4005698. PMID 24510101.
External links
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