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Phenolphthalein

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This article is about Phenolphthalein. For other related dyes in the phthalein family, see Phthalein dye.
Phenolphthalein
Names
Preferred IUPAC name
3,3-Bis(4-hydroxyphenyl)-2-benzofuran-1(3H)-one
Other names
3,3-Bis(4-hydroxyphenyl)isobenzofuran-1(3H)-one
Identifiers
3D model (JSmol)
ChEMBL
ChemSpider
DrugBank
ECHA InfoCard 100.000.914 Edit this at Wikidata
KEGG
UNII
  • InChI=1S/C20H14O4/c21-15-9-5-13(6-10-15)20(14-7-11-16(22)12-8-14)18-4-2-1-3-17(18)19(23)24-20/h1-12,21-22H checkY
    Key: KJFMBFZCATUALV-UHFFFAOYSA-N checkY
  • InChI=1/C20H14O4/c21-15-9-5-13(6-10-15)20(14-7-11-16(22)12-8-14)18-4-2-1-3-17(18)19(23)24-20/h1-12,21-22H
    Key: KJFMBFZCATUALV-UHFFFAOYAH
  • O=C1OC(C2=C1C=CC=C2)(C3=CC=C(C=C3)O[H])C4=CC=C(C=C4)O[H]
Properties
C20H14O4
Molar mass 318.328 g·mol−1
Appearance White powder
Density 1.277 g/cm3 (32 °C (90 °F))
Melting point 258–263 °C (496–505 °F; 531–536 K)[1]
400 mg/l
Solubility in other solvents Insoluble in benzene and hexane; very soluble in ethanol and ether; slightly soluble in DMSO
UV-vis (λmax) 552 nm (1st)
374 nm (2nd)[1]
Pharmacology
A06AB04 (WHO)
Hazards
GHS labelling:
GHS08: Health hazard[1]
Danger
H341, H350, H361[1]
P201, P281, P308+P313[1]
NFPA 704 (fire diamond)
NFPA 704 four-colored diamondHealth 2: Intense or continued but not chronic exposure could cause temporary incapacitation or possible residual injury. E.g. chloroformFlammability 3: Liquids and solids that can be ignited under almost all ambient temperature conditions. Flash point between 23 and 38 °C (73 and 100 °F). E.g. gasolineInstability 0: Normally stable, even under fire exposure conditions, and is not reactive with water. E.g. liquid nitrogenSpecial hazards (white): no code
2
3
0
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
☒N verify (what is checkY☒N ?)

Phenolphthalein (/fɛˈnɒl(f)θəliːn/[citation needed] feh-NOL(F)-thə-leen) is a chemical compound with the formula C20H14O4 and is often written as "HIn", "HPh", "phph" or simply "Ph" in shorthand notation. Phenolphthalein is often used as an indicator in acid–base titrations. For this application, it turns colorless in acidic solutions and pink in basic solutions. It belongs to the class of dyes known as phthalein dyes.

Phenolphthalein is slightly soluble in water and usually is dissolved in alcohols in experiments. It is a weak acid, which can lose H+ ions in solution. The nonionized phenolphthalein molecule is colorless and the double deprotonated phenolphthalein ion is fuchsia. Further proton loss in higher pH occurs slowly and leads to a colorless form. Phenolphthalein ion in concentrated sulfuric acid is orange red due to sulfonation.[2]

Uses

[edit]

pH indicator

[edit]
Phenolphthalein (pH indicator)
below pH 8.3 above pH 10.0
8.3 ⇌ 10.0

Phenolphthalein's common use is as an indicator in acid-base titrations. It also serves as a component of universal indicator, together with methyl red, bromothymol blue, and thymol blue.[3]

Phenolphthalein adopts different forms in aqueous solution depending on the pH of the solution.[4][2][5][6] Inconsistency exists in the literature about hydrated forms of the compounds and the color of sulfuric acid. Wittke reported in 1983 that it exists in protonated form (H3In+) under strongly acidic conditions, providing an orange coloration. However, a later paper suggested that this color is due to sulfonation to phenolsulfonphthalein.[2]

The lactone form (H2In) is colorless between strongly acidic and slightly basic conditions. The doubly deprotonated (In2-) phenolate form (the anion form of phenol) gives the familiar pink color. In strongly basic solutions, phenolphthalein is converted to its In(OH)3− form, and its pink color undergoes a rather slow fading reaction[6] and becomes completely colorless when pH is greater than 13.

The pKa values of phenolphthalein were found to be 9.05, 9.50 and 12 while those of phenolsulfonphthalein are 1.2 and 7.70.[2]

Species H3In+ H2In In2− In(OH)3−
Structure
Model
pH <−1 in H2SO4[4] 0–8.3 8.3–10.0[7] >12
Conditions strongly acidic acidic or near-neutral basic strongly basic
Color orange colorless pink to fuchsia colorless
Image
PP startAnimGif
An animation of the pH-dependent reaction mechanism: H3In+ → H2In → In2− → In(OH)3−

Carbonation of concrete

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Phenolphthalein's pH sensitivity is exploited in other applications: concrete has naturally high pH due to the calcium hydroxide formed when Portland cement reacts with water. As the concrete reacts with carbon dioxide in the atmosphere, pH decreases to 8.5–9. When a 1% phenolphthalein solution is applied to normal concrete, it turns bright pink. However, if it remains colorless, it shows that the concrete has undergone carbonation. In a similar application, some spackling used to repair holes in drywall contains phenolphthalein. When applied, the basic spackling material retains a pink color; when the spackling has cured by reaction with atmospheric carbon dioxide, the pink color fades.[8]

Education

[edit]

In a highly basic solution, phenolphthalein's slow change from pink to colorless as it is converted to its Ph(OH)3− form is used in chemistry classes for the study of reaction kinetics.

Entertainment

[edit]

Phenolphthalein is used in toys, for example as a component of disappearing inks, or disappearing dye on the "Hollywood Hair" Barbie hair. In the ink, it is mixed with sodium hydroxide, which reacts with carbon dioxide in the air. This reaction leads to the pH falling below the color change threshold as hydrogen ions are released by the reaction:

OH−(aq) + CO2(g) → CO2−
3(aq) + H+(aq).

To develop the hair and "magic" graphical patterns, the ink is sprayed with a solution of hydroxide, which leads to the appearance of the hidden graphics by the same mechanism described above for color change in alkaline solution. The pattern will eventually disappear again because of the reaction with carbon dioxide. Thymolphthalein is used for the same purpose and in the same way, when a blue color is desired.[9]

Detection of blood

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Main article: Kastle-Meyer test

A reduced form of phenolphthalein, phenolphthalin, which is colorless, is used in a test to identify substances thought to contain blood, commonly known as the Kastle–Meyer test. A dry sample is collected with a swab or filter paper. A few drops of alcohol, then a few drops of phenolphthalein, and finally a few drops of hydrogen peroxide are dripped onto the sample. If the sample contains hemoglobin, it will turn pink immediately upon addition of the peroxide, because of the generation of phenolphthalein. A positive test indicates the sample contains hemoglobin and, therefore, is likely blood. A false positive can result from the presence of substances with catalytic activity similar to hemoglobin. This test is not destructive to the sample; it can be kept and used in further tests. This test has the same reaction with blood from any animal whose blood contains hemoglobin, including almost all vertebrates; further testing would be required to determine whether it originated from a human.

Laxative

[edit]
See also: Phenol red

Phenolphthalein has been used for over a century as a laxative, but is now being removed from over-the-counter laxatives[10] over concerns of carcinogenicity.[11][12] Laxative products formerly containing phenolphthalein have often been reformulated with alternative active ingredients: Feen-a-Mint[13] switched to bisacodyl, and Ex-Lax[14] was switched to a senna extract.

Thymolphthalein is a related laxative made from thymol.

Despite concerns regarding its carcinogenicity based on rodent studies, the use of phenolphthalein as a laxative is unlikely to cause ovarian cancer.[15] Some studies suggest a weak association with colon cancer, while others show none at all.[16]

Phenolphthalein is described as a stimulant laxative.[16] In addition, it has been found to inhibit human cellular calcium influx via store-operated calcium entry (SOCE, see Calcium release activated channel § Structure) in vivo. This is effected by its inhibiting thrombin and thapsigargin, two activators of SOCE that increase intracellular free calcium.[17]

Phenolphthalein has been added to the European Chemicals Agency's candidate list for substance of very high concern (SVHC).[18] It is on the IARC group 2B list for substances "possibly carcinogenic to humans".[19]

The discovery of phenolphthalein's laxative effect was due to an attempt by the Hungarian government to label[clarification needed] genuine local white wine with the substance in 1900. Phenolphthalein did not change the taste of the wine and would change color when a base is added, making it a good label in principle. However, it was found that ingestion of the substance led to diarrhea. Max Kiss, a Hungarian-born pharmacist residing in New York, heard about the news and launched Ex-Lax in 1906.[20][19]

Synthesis

[edit]

Phenolphthalein can be synthesized by condensation of phthalic anhydride with two equivalents of phenol under acidic conditions. It was discovered in 1871 by Adolf von Baeyer.[21][22][23]

Synthesis of phenolphthalein[24]

See also

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References

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  1. ^ a b c d e "Phenolphthalein". Archived from the original on 8 November 2015. Retrieved 7 October 2014.
  2. ^ a b c d Tamura, Zenzo (December 1996). "Spectrophotometric Analysis of the Relationship between Dissociation and Coloration, and of the Structural Formulas of Phenolphthalein in Aqueous Solution". Analytical Sciences. 12 (6): 927–930. doi:10.2116/analsci.12.927.
  3. ^ "Universal Indicator". ISCID Encyclopedia of Science and Philosophy. Archived from the original on September 25, 2006.
  4. ^ a b Wittke, Georg (1 March 1983). "Reactions of phenolphthalein at various pH values". Journal of Chemical Education. 60 (3): 239. Bibcode:1983JChEd..60..239W. doi:10.1021/ed060p239. ISSN 0021-9584.
  5. ^ Abdel-Hamid, Refat (1 September 1998). "The Adsorption Behavior of Phenolphthalein at a Mercury Electrode in Water-Ethanol Solutions". Monatshefte für Chemie - Chemical Monthly. 129 (8): 817–826. doi:10.1007/PL00013491. S2CID 92339258.
  6. ^ a b Kunimoto, Ko-Ki (February 2001). "Molecular structure and vibrational spectra of phenolphthalein and its dianion". Spectrochimica Acta Part A. 57 (2): 265–271. Bibcode:2001AcSpA..57..265K. doi:10.1016/S1386-1425(00)00371-1. PMID 11206560.
  7. ^ Rathod, Balraj B.; Murthy, Sahana; Bandyopadhyay, Subhajit (20 February 2019). "Is this Solution Pink Enough? A Smartphone Tutor to Resolve the Eternal Question in Phenolphthalein-Based Titration". Journal of Chemical Education. 96 (3): 486–494. Bibcode:2019JChEd..96..486R. doi:10.1021/acs.jchemed.8b00708. S2CID 104475433.
  8. ^ US 6531528, Ronald D. Kurp, "Ready to use spackle/repair product containing dryness indicator", published 2003-03-11, assigned to Dap Products Inc. 
  9. ^ "Toystore" (PDF). Archived (PDF) from the original on 2021-07-02. Retrieved 2022-02-22.
  10. ^ Spiller, H. A.; Winter, M. L.; Weber, J. A.; Krenzelok, E. P.; Anderson, D. L.; Ryan, M. L. (May 2003). "Skin Breakdown and Blisters from Senna-Containing Laxatives in Young Children". The Annals of Pharmacotherapy. 37 (5): 636–639. doi:10.1345/aph.1C439. PMID 12708936. S2CID 31837142.
  11. ^ Dunnick, J. K.; Hailey, J. R. (1996). "Phenolphthalein Exposure Causes Multiple Carcinogenic Effects in Experimental Model Systems" (PDF). Cancer Research. 56 (21): 4922–4926. PMID 8895745. Archived (PDF) from the original on 2022-02-17. Retrieved 2022-02-22.
  12. ^ Tice, R. R.; Furedi-Machacek, M.; Satterfield, D.; Udumudi, A.; Vasquez, M.; Dunnick, J. K. (1998). "Measurement of Micronucleated Erythrocytes and DNA Damage during Chronic Ingestion of Phenolphthalein in Transgenic Female Mice Heterozygous for the p53 Gene". Environmental and Molecular Mutagenesis. 31 (2): 113–124. doi:10.1002/(SICI)1098-2280(1998)31:2<113::AID-EM3>3.0.CO;2-N. PMID 9544189. S2CID 36550267.
  13. ^ "Phenolphthalein". DrugBank. Archived from the original on 2020-08-06. Retrieved 2020-04-25.
  14. ^ Stolberg, Sheryl Gay (August 30, 1997). "3 Versions of Ex-Lax Are Recalled After F.D.A. Proposes Ban on Ingredient". The New York Times. Archived from the original on May 7, 2021. Retrieved April 25, 2020.
  15. ^ Cooper, G. S.; Longnecker, M. P.; Peters, R. K. (2004). "Ovarian Cancer Risk and Use of Phenolphthalein-Containing Laxatives". Pharmacoepidemiology and Drug Safety. 13 (1): 35–39. doi:10.1002/pds.824. PMID 14971121. S2CID 24783082. Archived from the original on 2022-01-26. Retrieved 2019-06-26.
  16. ^ a b "Other pharmaceutical agents.". IARC monographs on the evaluation of carcinogenic risks to humans. Vol. 76. International Agency for Research on Cancer. 2000. pp. 345–486. PMC 7196975. PMID 11000977.
  17. ^ Dobrydneva, Y.; Wilson, E.; Abelt, C. J.; Blackmore, P. F. (2009). "Phenolphthalein as a Prototype Drug for a Group of Structurally Related Calcium Channel Blockers in Human Platelets". Journal of Cardiovascular Pharmacology. 53 (3): 231–240. doi:10.1097/FJC.0b013e31819b5494. PMID 19247192. S2CID 21833487.
  18. ^ "Phenolphthalein - Substance Information - ECHA". echa.europa.eu. Archived from the original on 2018-06-12. Retrieved 2017-11-06.
  19. ^ a b Arney, Kat (2018-10-25). "Phenolphthalein". Chemistry World.
  20. ^ May, Paul. "Phenolphthalein - Molecule of the Month - March 2022 (HTML version)". www.chm.bris.ac.uk.
  21. ^ Baeyer, A. (1871). "Ueber eine neue Klasse von Farbstoffen". Berichte der Deutschen Chemischen Gesellschaft. 4 (2): 555–558. doi:10.1002/cber.18710040209. Archived from the original on 2021-02-14. Retrieved 2020-09-02.
  22. ^ Baeyer, A. (1871). "Ueber die Phenolfarbstoffe". Berichte der Deutschen Chemischen Gesellschaft. 4 (2): 658–665. doi:10.1002/cber.18710040247. Archived from the original on 2021-10-11. Retrieved 2019-06-26.
  23. ^ Baeyer, A. (1871). "Ueber die Phenolfarbstoffe". Polytechnisches Journal. 201 (89): 358–362. Archived from the original on 2021-10-11. Retrieved 2022-02-22.
  24. ^ Max Hubacher, U.S. patent 2,192,485 (1940 to Ex Lax Inc)
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