Molybdopterin synthase sulfurtransferase

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Molybdopterin synthase sulfurtransferase
Molybdopterin synthase sulfurtransferase
Identifiers
EC no.	2.8.1.11
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
PMC
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PubMed	articles
NCBI	proteins

Molybdopterin synthase sulfurtransferase (EC 2.8.1.11, adenylyltransferase and sulfurtransferase MOCS3, Cnx5 (gene), molybdopterin synthase sulfurylase) is an enzyme with systematic name persulfurated L-cysteine desulfurase:(molybdopterin-synthase sulfur-carrier protein)-Gly-Gly sulfurtransferase.^[1]^[2]^[3]^[4] This enzyme catalyses the following chemical reaction

[molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP + [cysteine desulfurase]-S-sulfanyl-L-cysteine

\rightleftharpoons

AMP + [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + cysteine desulfurase

The enzyme transfers sulfur to form a thiocarboxylate moiety on the C-terminal glycine of the small subunit of molybdopterin synthase.

References

^ Matthies A, Nimtz M, Leimkühler S (May 2005). "Molybdenum cofactor biosynthesis in humans: identification of a persulfide group in the rhodanese-like domain of MOCS3 by mass spectrometry". Biochemistry. 44 (21): 7912–20. doi:10.1021/bi0503448. PMID 15910006.
^ Leimkühler S, Rajagopalan KV (June 2001). "A sulfurtransferase is required in the transfer of cysteine sulfur in the in vitro synthesis of molybdopterin from precursor Z in Escherichia coli". The Journal of Biological Chemistry. 276 (25): 22024–31. doi:10.1074/jbc.M102072200. PMID 11290749.
^ Hänzelmann P, Dahl JU, Kuper J, Urban A, Müller-Theissen U, Leimkühler S, Schindelin H (December 2009). "Crystal structure of YnjE from Escherichia coli, a sulfurtransferase with three rhodanese domains". Protein Science. 18 (12): 2480–91. doi:10.1002/pro.260. PMC 2821267. PMID 19798741.
^ Dahl JU, Urban A, Bolte A, Sriyabhaya P, Donahue JL, Nimtz M, Larson TJ, Leimkühler S (October 2011). "The identification of a novel protein involved in molybdenum cofactor biosynthesis in Escherichia coli". The Journal of Biological Chemistry. 286 (41): 35801–12. doi:10.1074/jbc.M111.282368. PMC 3195606. PMID 21856748.

External links

Molybdopterin+synthase+sulfurtransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

[1] Matthies A, Nimtz M, Leimkühler S (May 2005). "Molybdenum cofactor biosynthesis in humans: identification of a persulfide group in the rhodanese-like domain of MOCS3 by mass spectrometry". Biochemistry. 44 (21): 7912–20. doi:10.1021/bi0503448. PMID 15910006.

[2] Leimkühler S, Rajagopalan KV (June 2001). "A sulfurtransferase is required in the transfer of cysteine sulfur in the in vitro synthesis of molybdopterin from precursor Z in Escherichia coli". The Journal of Biological Chemistry. 276 (25): 22024–31. doi:10.1074/jbc.M102072200. PMID 11290749.

[3] Hänzelmann P, Dahl JU, Kuper J, Urban A, Müller-Theissen U, Leimkühler S, Schindelin H (December 2009). "Crystal structure of YnjE from Escherichia coli, a sulfurtransferase with three rhodanese domains". Protein Science. 18 (12): 2480–91. doi:10.1002/pro.260. PMC 2821267. PMID 19798741.

[4] Dahl JU, Urban A, Bolte A, Sriyabhaya P, Donahue JL, Nimtz M, Larson TJ, Leimkühler S (October 2011). "The identification of a novel protein involved in molybdenum cofactor biosynthesis in Escherichia coli". The Journal of Biological Chemistry. 286 (41): 35801–12. doi:10.1074/jbc.M111.282368. PMC 3195606. PMID 21856748.

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v t e Transferases: sulfur-containing group (EC 2.8)
2.8.1: Sulfurtransferases	Thiosulfate sulfurtransferase Rhodanese 3-mercaptopyruvate sulfurtransferase thiosulfate—thiol sulfurtransferase tRNA sulfurtransferase thiosulfate—dithiol sulfurtransferase biotin synthase cysteine desulfurase lipoyl synthase molybdenum cofactor sulfurtransferase thiazole synthase molybdopterin synthase sulfurtransferase molybdopterin synthase tRNA-uridine 2-sulfurtransferase () tRNA-5-taurinomethyluridine 2-sulfurtransferase () tRNA-5-methyluridine⁵⁴ 2-sulfurtransferase () L-aspartate semialdehyde sulfurtransferase ()
2.8.2: Sulfotransferases	aryl sulfotransferase amine sulfotransferase estrone sulfotransferase chondroitin 4-sulfotransferase choline sulfotransferase UDP-N-acetylgalactosamine-4-sulfate sulfotransferase heparan sulfate-glucosamine N-sulfotransferase tyrosine-ester sulfotransferase Renilla-luciferin sulfotransferase galactosylceramide sulfotransferase psychosine sulfotransferase bile salt sulfotransferase steroid sulfotransferase thiol sulfotransferase chondroitin 6-sulfotransferase cortisol sulfotransferase triglucosylalkylacylglycerol sulfotransferase protein-tyrosine sulfotransferase keratan sulfotransferase aryl-sulfate sulfotransferase (heparan sulfate)-glucosamine 3-sulfotransferase 1 desulfoglucosinolate sulfotransferase flavonol 3-sulfotransferase quercetin-3-sulfate 3′-sulfotransferase quercetin-3-sulfate 4′-sulfotransferase quercetin-3,3′-bissulfate 7-sulfotransferase (heparan sulfate)-glucosamine 3-sulfotransferase 2 (heparan sulfate)-glucosamine 3-sulfotransferase 3 petromyzonol sulfotransferase scymnol sulfotransferase N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase glycochenodeoxycholate sulfotransferase dermatan 4-sulfotransferase desulfo-A47934 sulfotransferase () trehalose 2-sulfotransferase () aliphatic desulfoglucosinolate sulfotransferase () hydroxyjasmonate sulfotransferase () ω-hydroxy-β-dihydromenaquinone-9 sulfotransferase (*) Alcohol sulfotransferase Tyrosylprotein sulfotransferase
2.8.3: CoA-transferases	Propionate CoA-transferase oxalate CoA-transferase malonate CoA-transferase 3-oxoacid CoA-transferase 3-oxoadipate CoA-transferase succinyl-CoA—Dcitramalate CoA-transferase acetate CoA-transferase butyrate—acetoacetate CoA-transferase citrate CoA-transferase citramalate CoA-transferase glutaconate CoA-transferase succinate—hydroxymethylglutarate CoA-transferase 5-hydroxypentanoate CoA-transferase succinyl-CoA:(R)-benzylsuccinate CoA-transferase formyl-CoA transferase cinnamoyl-CoA:phenyllactate CoA-transferase succinyl-CoA:acetate CoA-transferase () CoA:oxalate CoA-transferase () succinyl-CoA—D-citramalate CoA-transferase () L-carnitine CoA-transferase () succinyl-CoA—L-malate CoA-transferase () caffeate CoA-transferase () (R)-2-hydroxy-4-methylpentanoate CoA-transferase () bile acid CoA-transferase () succinyl-CoA:mesaconate CoA transferase (*)
2.8.4: Alkylthio	2.8.4: Coenzyme-B sulfoethylthiotransferase arsenate-mycothiol transferase tRNA-2-methylthio-N⁶-dimethylallyladenosine synthase () [ribosomal protein S12] (aspartate⁸⁹-C³)-methylthiotransferase () tRNA (N⁶-L-threonylcarbamoyladenosine³⁷-C²)-methylthiotransferase (*)
2.8.5:	2.8.5: S-sulfo-L-cysteine synthase (3-phospho-L-serine-dependent) () L-cysteine S-thiosulfotransferase ()

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)