List of restriction enzyme cutting sites: Ba–Bc
Appearance
Legend of nucleobases | |
---|---|
Code | Nucleotide represented |
A | Adenine (A) |
C | Cytosine (C) |
G | Guanine (G) |
T | Thymine (T) |
N | A, C, G or T |
M | A or C |
R | A or G |
W | A or T |
Y | C or T |
S | C or G |
K | G or T |
H | A, C or T |
B | C, G or T |
V | A, C or G |
D | A, G or T |
This article contains a list of the most studied restriction enzymes whose names start with Ba to Bc inclusive. It contains approximately 120 enzymes.
The following information is given:
- Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature[1][2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
- PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action[3][4].
- Source: Organism that naturally produces the enzyme.
- Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
- Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site[5][6].
- Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:
as in this not listed enzyme: EcoR70I
Whole list navigation
[edit]Restriction enzymes
[edit]Ba - Bc
[edit]Enzyme | PDB code | Source | Recognition sequence | Cut | Isoschizomers | ||||||
Bac36I | Bacillus alcalophilus 36 | 5' GGNCC 3' CCNGG
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5' ---G GNCC--- 3' 3' ---CCNG G--- 5'
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AspS9I, AvcI, BavAII, Bce22I, Bsp1894I, Bsu54I, FmuI, NspIV | |||||||
BaeI[7] | Bacillus sphaericus | 5' ACN4GTAYC 3' TGN4CATYG
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5' ---ACN4GTAYCN6NNNNNN --- 3' 3' ---TGN4CATYGN6N NNNNN--- 5'
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— None in May 2010 — | |||||||
BalI[8][9] | Brevibacterium albidum | 5' TGGCCA 3' ACCGGT
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5' ---TGG CCA--- 3' 3' ---ACC GGT--- 5'
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Bal228I | Bacillus alcalophilus 228 | 5' GGNCC 3' CCNGG
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5' ---G GNCC--- 3' 3' ---CCNG G--- 5'
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AspS9I, AvcI, BavAII, BshKI, Bsp1894I, Bsu54I, FmuI, NspIV | |||||||
BamHI[10][11][12][13][14][15][16][17][18][19][20] [21][22][23][24][25][26][27][28][29][30] |
1BAM | Bacillus amyloliquefaciens H | 5' GGATCC 3' CCTAGG
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5' ---G GATCC--- 3' 3' ---CCTAG G--- 5'
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AccEBI, AliI, ApaCI, AsiI, Bce751I, Bsp98I, Bsp4009I, BspAAIII, CelI, Nsp29132II, NspSAIV, SolI, SurI | ||||||
BamNII | Bacillus amyloliquefaciens N | 5' GGWCC 3' CCWGG
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5' ---G GWCC--- 3' 3' ---CCWG G--- 5'
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BcuAI, BsrAI, CauI, EagMI, FdiI, HgiCII, HgiJI, SinI | |||||||
BanI[31][32] | Bacillus aneurinolyticus | 5' GGYRCC 3' CCRYGG
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5' ---G GYRCC--- 3' 3' ---CCRYG G--- 5'
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AccB1I, BbvBI, BspT107I, Eco64I, HgiCI, HgiHI, MspB4I, PfaAI | |||||||
BanII[12][31] | Bacillus aneurinolyticus | 5' GRGCYC 3' CYCGRG
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5' ---GRGCY C--- 3' 3' ---C YCGRG--- 5'
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BanIII[31] | Bacillus aneurinolyticus | 5' ATCGAT 3' TAGCTA
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5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5'
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AagI, BavCI, Bsa29I, BseCI, BspDI, Bsu15I, BsuTUI, ClaI | |||||||
BanAI | Bacillus anthracis | 5' GGCC 3' CCGG
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5' ---GG CC--- 3' 3' ---CC GG--- 5'
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BasI | Bacillus sp. | 5' CCAN5TGG 3' GGTN5ACC
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5' ---CCANNNN NTGG--- 3' 3' ---GGTN NNNNACC--- 5'
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AccB7I, AcpII, Asp10HII, Esp1396I, PflBI, PflMI, Van91I | |||||||
BauI | Bacillus aquaemaris RFL1 | 5' CACGAG 3' GTGCTC
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5' ---C ACGAG--- 3' 3' ---GTGCT C--- 5'
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BavI | Bacillus alvei | 5' CAGCTG 3' GTCGAC
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5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5'
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BavAI | Bacillus alvei A | 5' CAGCTG 3' GTCGAC
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5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5'
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BavAII | Bacillus alvei A | 5' GGNCC 3' CCNGG
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5' ---G GNCC--- 3' 3' ---CCNG G--- 5'
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AspS9I, AvcI, BavBII, BshKI, Bsp1894I, Bsu54I, FmuI, NspIV | |||||||
BavBI | Bacillus alvei B | 5' CAGCTG 3' GTCGAC
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5' ---CAG CTG--- 3' 3' ---GTC GAC--- 5'
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BavBII | Bacillus alvei B | 5' GGNCC 3' CCNGG
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5' ---G GNCC--- 3' 3' ---CCNG G--- 5'
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AspS9I, Bac36I, BavAII, BshKI, BspBII, Bsu54I, FmuI, Pde12I | |||||||
BavCI | Bacillus alvei C | 5' ATCGAT 3' TAGCTA
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5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5'
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AagI, BanIII, Bsa29I, BseCI, BspDI, Bsu15I, BsuTUI, ClaI | |||||||
BbeI[33] | Bifidobacterium breve YIT4006 | 5' GGCGCC 3' CCGCGG
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5' ---GGCGC C--- 3' 3' ---C CGCGG--- 5'
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BbiII | Bifidobacterium bifidum YIT4007 | 5' GRCGYC 3' CYGCRG
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5' ---GR CGYC--- 3' 3' ---CYGC RG--- 5'
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AcyI, AhaII, BbiII, HgiHII, Hin1I, Hsp92I, Msp17I, PamII | |||||||
Bbi24I | Bifidobacterium bifidum S-24 | 5' ACGCGT 3' TGCGCA
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5' ---A CGCGT--- 3' 3' ---TGCGC A--- 5'
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BbrI | Bordetella bronchiseptica 4994 | 5' AAGCTT 3' TTCGAA
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5' ---A AGCTT--- 3' 3' ---TTCGA A--- 5'
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Bbr7I | Bacillus brevis 7 | 5' GAAGAC 3' CTTCTG
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5' ---GAAGACN6N NNNNN--- 3' 3' ---CTTCTGN6NNNNNN --- 5'
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BbrPI[34][35] | Bacillus brevis | 5' CACGTG 3' GTGCAC
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5' ---CAC GTG--- 3' 3' ---GTG CAC--- 5'
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AcvI, BcoAI, Eco72I, PmaCI, PmlI, PspCI | |||||||
BbsI[36] | Bacillus laterosporus | 5' GAAGAC 3' CTTCTG
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5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5'
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BbuI[37] | Bacillus circulans | 5' GCATGC 3' CGTACG
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5' ---GCATG C--- 3' 3' ---C GTACG--- 5'
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BbvI[38][39] | Bacillus brevis | 5' GCAGC 3' CGTCG
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5' ---GCAGCN7N NNNN--- 3' 3' ---CGTCGN7NNNNN --- 5'
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AlwXI, BseKI, BseXI, Bsp423I, Bst12I, Bst71I, BstV1I | |||||||
BbvII[40] | Bacillus brevis 80 | 5' GAAGAC 3' CTTCTG
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5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5'
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Bbv12I[41] | Bacillus brevis 12 | 5' GWGCWC 3' CWCGWG
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5' ---GWGCW C--- 3' 3' ---C WCGWG--- 5'
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Bbv16II | Bacillus brevis 16 | 5' GAAGAC 3' CTTCTG
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5' ---GAAGACNN NNNN--- 3' 3' ---CTTCTGNNNNNN --- 5'
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BbvAI | Bacillus brevis A | 5' GAAN4TTC 3' CTTN4AAG
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5' ---GAANN NNTTC--- 3' 3' ---CTTNN NNAAG--- 5'
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Asp700I, MroXI, PdmI, XmnI | |||||||
BbvAII | Bacillus brevis A | 5' ATCGAT 3' TAGCTA
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5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5'
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BavCI, Bci29I, Bli86I, BseCI, BspZEI, Bsu15I, ClaI, Rme21I | |||||||
BbvAIII | Bacillus brevis A | 5' TCCGGA 3' AGGCCT
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5' ---T CCGGA--- 3' 3' ---AGGCC T--- 5'
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AccIII, Aor13HI, BlfI, BseAI, Bsp13I, BspEI, Bsu23I, Kpn2I | |||||||
BbvBI | Bacillus brevis B | 5' GGYRCC 3' CCRYGG
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5' ---G GYRCC--- 3' 3' ---CCRYG G--- 5'
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BanI, BshNI, BspT107I, Eco64I, HgiCI, HgiHI, MspB4I, PfaAI | |||||||
BbvCI[42] | Bacillus brevis | 5' CCTCAGC 3' GGAGTCG
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5' ---CC TCAGC--- 3' 3' ---GGAGT CG--- 5'
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AbeI | |||||||
Bca77I | Bacillus caldolyticus | 5' WCCGGW 3' WGGCCW
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5' ---W CCGGW--- 3' 3' ---WGGCC W--- 5'
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BccI | Bacteroides caccae | 5' CCATC 3' GGTAG
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5' ---CCATCNNNN N--- 3' 3' ---GGTAGNNNNN --- 5'
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Bce4I | Bacillus cereus B4 | 5' GCN7GC 3' CGN7CG
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5' ---GCNNNNN NNGC--- 3' 3' ---CGNN NNNNNCG--- 5'
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Bce22I | Bacillus cereus 22 | 5' GGNCC 3' CCNGG
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5' ---G GNCC--- 3' 3' ---CCNG G--- 5'
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AspS9I, Bac36I, BavAII, BshKI, BspBII, CcuI, FmuI, Pde12I | |||||||
Bce83I[43] | Bacillus cereus 83 | 5' CTTGAG 3' GAACTC
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5' ---CTTGAGN13NNN --- 3' 3' ---GAACTCN13N NN--- 5'
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Bce243I | Bacillus cereus | 5' GATC 3' CTAG
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5' --- GATC--- 3' 3' ---CTAG --- 5'
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Bfi57I, Bsp143I, BspJI, BstMBI, CviAI, Kzo9I, NdeII, Sth368I | |||||||
Bce751I | Bacillus cereus 751 | 5' GGATCC 3' CCTAGG
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5' ---G GATCC--- 3' 3' ---CCTAG G--- 5'
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BamHI, Bce751I, BnaI, Bsp98I, Bsp4009I, BstI, NspSAIV, Pfl8I | |||||||
BceAI | Bacillus cereus 1315 | 5' ACGGC 3' TGCCG
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5' ---ACGGCN11N NN--- 3' 3' ---TGCCGN11NNN --- 5'
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BceBI | Bacillus cereus 1323 | 5' CGCG 3' GCGC
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5' ---CG CG--- 3' 3' ---GC GC--- 5'
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AccII, Bsh1236I, BtkI, Csp68KVI, FalII, FauBII, FnuDII, SelI, ThaI | |||||||
BceCI | Bacillus cereus 1195 | 5' GCN7GC 3' CGN7CG
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5' ---GCNNNNN NNGC--- 3' 3' ---CGNN NNNNNCG--- 5'
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BcefI[44] | Bacillus cereus fluorescens | 5' ACGGC 3' TGCCG
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5' ---ACGGCN10NN N--- 3' 3' ---TGCCGN10NNN --- 5'
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BcgI[45][46] | Bacillus coagulans | 5' CGAN6TGC 3' GCTN6ACG
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5' ---CGAN6TGCN9NNN --- 3' 3' ---GCTN6ACGN9N NN--- 5'
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Bci29I | Bacillus circulans 29 | 5' ATCGAT 3' TAGCTA
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5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5'
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BavCI, BciBI, Bli86I, BseCI, BspZEI, Bsu15I, ClaI, Rme21I | |||||||
BciBI | Bacillus circulans B | 5' ATCGAT 3' TAGCTA
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5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5'
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BavCI, BcmI, Bli86I, BseCI, BspZEI, Bsu15I, ClaI, Rme21I | |||||||
BciBII | Bacillus circulans B | 5' CCWGG 3' GGWCC
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5' ---CC WGG--- 3' 3' ---GGW CC--- 5'
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AjnI, ApyI, BptI, Bst1I, BstOI, BstM6I, Bst2UI, EcoRII, MvaI | |||||||
BciVI[47] | Bacillus circulans | 5' GTATCC 3' CATAGG
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5' ---GTATCCN4NN --- 3' 3' ---CATAGGN4N N--- 5'
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BfuI | |||||||
BclI[15][48][49] | Bacillus caldolyticus | 5' TGATCA 3' ACTAGT
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5' ---T GATCA--- 3' 3' ---ACTAG T--- 5'
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BcmI | Bacillus sp. | 5' ATCGAT 3' TAGCTA
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5' ---AT CGAT--- 3' 3' ---TAGC TA--- 5'
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BsuTUI, Bsu15I, BavCI, Bli86I, BspZEI, Rme21I, BseCI, BdiI | |||||||
BcnI[50][51][52] | 2ODH | Bacillus centrosporus RFL1 | 5' CCSGG 3' GGSCC
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5' ---CC SGG--- 3' 3' ---GGS CC--- 5'
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BcoI[53] | Bacillus coagulans SM 1 | 5' CYCGRG 3' GRGCYC
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5' ---C YCGRG--- 3' 3' ---GRGCY C--- 5'
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Ama87I, AquI, BsoBI, BstSI, Eco88I, NspSAI, OfoI, PunAI | |||||||
Bco5I | Bacillus coagulans 5 | 5' CTCTTC 3' GAGAAG
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5' ---CTCTTCN NNN--- 3' 3' ---GAGAAGNNNN --- 5'
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Bco27I | Bacillus coagulans 27 | 5' CCGG 3' GGCC
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5' ---C CGG--- 3' 3' ---GGC C--- 5'
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Bco116I | Bacillus coagulans 116 | 5' CTCTTC 3' GAGAAG
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5' ---CTCTTCN NNN--- 3' 3' ---GAGAAGNNNN --- 5'
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Bco118I | Bacillus coagulans 118 | 5' RCCGGY 3' YGGCCR
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5' ---R CCGGY--- 3' 3' ---YGGCC R--- 5'
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BcoAI | Bacillus coagulans AUCM B-732 | 5' CACGTG 3' GTGCAC
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5' ---CAC GTG--- 3' 3' ---GTG CAC--- 5'
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AcvI, BbrPI, Eco72I, PmaCI, PmlI, PspCI | |||||||
BcoKI | Bacillus coagulans | 5' CTCTTC 3' GAGAAG
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5' ---CTCTTCN NNN--- 3' 3' ---GAGAAGNNNN --- 5'
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BcuI | Bacillus coagulans Vs 29-022 | 5' ACTAGT 3' TGATCA
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5' ---A CTAGT--- 3' 3' ---TGATC A--- 5'
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AhII, AclNI, SpeI | |||||||
BcuAI | Bacillus cereus BKM B-814 | 5' GGWCC 3' CCWGG
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5' ---G GWCC--- 3' 3' ---CCWG G--- 5'
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BamNxI, BsrAI, Csp68KI, EagMI, FssI, HgiCII, HgiJI, SinI |
Notes
[edit]- ^ Smith HO, Nathans D (December 1973). "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23. doi:10.1016/0022-2836(73)90152-6. PMID 4588280.
- ^ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SK, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY (April 2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7): 1805–12. doi:10.1093/nar/gkg274. PMC 152790. PMID 12654995.
- ^ Jeremy MB, John LT, Lubert S (2002). "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. ISBN 0-7167-4684-0.
- ^ Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science. 181 (4096): 223–30. doi:10.1126/science.181.4096.223. PMID 4124164.
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- ^ Pingoud A, Jeltsch A (September 2001). "Structure and function of type II restriction endonucleases". Nucleic Acids Res. 29 (18): 3705–27. doi:10.1093/nar/29.18.3705. PMC 55916. PMID 11557805.
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- ^ Nelson PS, Papas TS, Schweinfest CW (February 1993). "Restriction endonuclease cleavage of 5-methyl-deoxycytosine hemimethylated DNA at high enzyme-to-substrate ratios". Nucleic Acids Res. 21 (3): 681–6. doi:10.1093/nar/21.3.681. PMC 309169. PMID 8441677.
- ^ Greene PJ, Heyneker HL, Bolivar F, Rodriguez RL, Betlach MC, Covarrubias AA, Backman K, Russel DJ, Tait R, Boyer HW (July 1978). "A general method for the purification of restriction enzymes". Nucleic Acids Res. 5 (7): 2373–80. doi:10.1093/nar/5.7.2373. PMC 342170. PMID 673857.
- ^ a b Huang LH, Farnet CM, Ehrlich KC, Ehrlich M (March 1982). "Digestion of highly modified bacteriophage DNA by restriction endonucleases". Nucleic Acids Res. 10 (5): 1579–91. doi:10.1093/nar/10.5.1579. PMC 320551. PMID 6280151.
- ^ Wilson GA, Young FE (September 1975). "Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens H". J Mol Biol. 97 (1): 123–5. doi:10.1016/S0022-2836(75)80028-3. PMID 1177312.
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- ^ Brooks JE, Benner JS, Heiter DF, Silber KR, Sznyter LA, Jager-Quinton T, Moran LS, Slatko BE, Wilson GG, Nwankwo DO (February 1989). "Cloning the BamHI restriction modification system". Nucleic Acids Res. 17 (3): 979–97. doi:10.1093/nar/17.3.979. PMC 331717. PMID 2537955.
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- ^ Newman M, Strzelecka T, Dorner LF, Schildkraut I, Aggarwal AK (April 1994). "Structure of restriction endonuclease BamHI and its relationship to EcoRI". Nature. 368 (6472): 660–4. Bibcode:1994Natur.368..660N. doi:10.1038/368660a0. PMID 8145855. S2CID 2999008.
- ^ Roy KB, Vrushank D, Jayaram B (July 1994). "Use of isotope-dilution phenomenon to advantage in the determination of kinetic constants Km and Kcat for BamHI restriction endonuclease: an empirical and iterative approach". Anal Biochem. 220 (1): 160–4. doi:10.1006/abio.1994.1313. PMID 7978240.
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