Jump to content

Lacto-N-biosidase

From Wikipedia, the free encyclopedia
Lacto-N-biosidase
Identifiers
EC no.3.2.1.140
CAS no.146359-52-6
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Search
PMCarticles
PubMedarticles
NCBIproteins

Lacto-N-biosidase (EC 3.2.1.140) is an enzyme with systematic name oligosaccharide lacto-N-biosylhydrolase.[1][2] This enzyme catalyses the following chemical reaction

(1) beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc + H2O beta-D-Gal-(1->3)-D-GlcNAc + beta-D-Gal-(1->4)-D-Glc
(2) lacto-N-tetraose + H2O lacto-N-biose + lactose

The enzyme from Streptomyces specifically hydrolyses the terminal lacto-N-biosyl residue (beta-D-Gal-(1->3)-D-GlcNAc) from the non-reducing end of oligosaccharides with the structure beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->R).

References

[edit]
  1. ^ Sano M, Hayakawa K, Kato I (September 1992). "An enzyme releasing lacto-N-biose from oligosaccharides". Proceedings of the National Academy of Sciences of the United States of America. 89 (18): 8512–8516. doi:10.1073/pnas.89.18.8512. PMC 49950. PMID 1528855.
  2. ^ Sano M, Hayakawa K, Kato I (September 1993). "Purification and characterization of an enzyme releasing lacto-N-biose from oligosaccharides with type 1 chain". The Journal of Biological Chemistry. 268 (25): 18560–18566. doi:10.1016/S0021-9258(17)46664-6. PMID 7689556.
[edit]