Intron-encoded endonuclease I-SceI

Intron-encoded endonuclease I-SceI
Identifiers
Organism	Saccharomyces cerevisiae S288C
Symbol	SCEI
PDB	1R7M
UniProt	P03882
Search for Structures Swiss-model Domains InterPro

Intron-encoded endonuclease I-Sce I is a homing endonuclease. The enzyme is used in biotechnology as a meganuclease. It recognises an 18-base pair sequence TAGGGATAACAGGGTAAT and leaves a 4 base pair 3' hydroxyl overhang. It is a rare cutting endonuclease. Statistically an 18-bp sequence will occur once in every 6.9*10¹⁰ base pairs (a frequency of 1 in 4¹⁸). This sequence does not normally occur in a human or mouse genome.

Sources

I-SceI is coded by introns. It is present in the mitochondria of yeast Saccharomyces cerevisiae.