Hypomagnesemia with secondary hypocalcemia
| Hypomagnesemia with secondary hypocalcemia | |
|---|---|
| Other names | Familial primary hypomagnesemia with hypocalcuria |
| Specialty | Endocrinology |
Hypomagnesemia with secondary hypocalcemia (HSH) is an autosomal recessive genetic disorder that affects the absorption of magnesium in the intestines. It is characterized by reduced reabsorption of magnesium from our diet in the intestines, leading to decreased levels of magnesium in the bloodstream. This, in turn, causes a decrease in the production of parathyroid hormone (PTH) by the parathyroid gland. Consequently, there is a decrease in both PTH and serum calcium levels, resulting in secondary hypocalcemia.
One of the main symptoms of HSH is the occurrence of convulsions and spasms in early infancy. If left untreated, these symptoms can potentially lead to intellectual disability or even death. HSH is primarily caused by mutations in the TRPM6 gene, which plays a crucial role in maintaining the balance of magnesium in the body.
Presentation
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Pathophysiology
[edit]HSH is primarily caused by a reduction in intestinal magnesium reabsorption. Intestinal magnesium reabsorption primarily occurs by membrane transport through the TRPM6 ion channels and is crucial for magnesium homeostasis. TRPM6 is expressed on the part of the cell membrane facing the intestinal lumen on intestinal cells, known as enterocytes. It acts as an ion channel to allow the ions (charged atoms) of magnesium (Mg2+) and calcium (Ca2+) to flow into the cell. It generates “outwardly-rectifying" currents, and as a result a positive charge will then pass more easily in the outward direction, out the cell, than in the inward direction, into the cell. The outward portion of these currents consist of sodium (Na+), while the inward portion is composed of the divalent cations: magnesium and calcium. The entry of sodium ions is blocked by extracellular divalent cations. Additionally, increased levels of intracellular magnesium lead to a decrease in current through TRPM6 channels.[1]
More than 30 mutations in the TRPM6 gene have been identified as being associated with HSH. These mutations are scattered throughout the gene (refer to Table 1). Out of the eight HSH mutations that have been tested, none have been shown to produce whole-cell current. One notable missense mutation, S141L, inhibits coassembly with TRPM7, as well as other TRPM6 subunits, and fails to allow traffic of the channel to the cell membrane. The trafficking ability and coassembly of other mutant forms of TRPM6 have yet to be extensively studied and require further investigation.[citation needed]
While hypomagnesemia in patients with HSH directly results from TRPM6 mutations, hypocalcemia is an indirect and secondary consequence. Decreased serum magnesium levels result to reduced the secretion of parathyroid hormone (PTH) by the parathyroid gland. PTH plays a vital role in regulating serum calcium levels. Decreased levels of PTH result in a decrease in the availability of calcium in the bloodstream, which contributes to the neurological symptoms observed in HSH.[citation needed]
| Mutation | Location | Functional? | Reference | |
|---|---|---|---|---|
| Nucleotide | Amino acid | |||
| c.C166T | R56X | N-terminus | [2] | |
| c.C422T | S141L | N-terminus | No | [3],[4],[5],[6] |
| c.G469T | E157X | N-terminus | [5] | |
| c.T521G | I174R | N-terminus | [7] | |
| c.668delA | D223fsX263 | N-terminus | [5] | |
| c.1010+5G→C | Splicing | N-terminus | [2] | |
| c.A1060C | T354P | N-terminus | [7] | |
| c.1134+5G→A | Splicing | N-terminus | [7] | |
| c.1208-1G→A | Splicing | N-terminus | [5] | |
| c.1280delA | H427fsX429 | N-terminus | No | [3],[5] |
| c.1308+1G→A | Splicing | N-terminus | [5] | |
| c.C1437A | Y479X | N-terminus | [7] | |
| c.C1450T | R484X | N-terminus | [2] | |
| c.C1769G | S590X | N-terminus | No | [3],[5],[8] |
| c.del1796-1797 | P599fsX609 | N-terminus | [5] | |
| c.2009+1G→A | Splicing | N-terminus | [2],[7] | |
| c.G2120A | C707Y | N-terminus | [7] | |
| c.2207delG | R736fsX737 | N-terminus | No | [3],[5],[8] |
| c.2537-2A→T | Splicing | N-terminus | [5] | |
| c.2667+1G→A | Splicing | [3],[5] | ||
| c.C2782T | R928X | M3 | No | [5] |
| c.del Ex 21 | M4 | [5] | ||
| c.Del2831-2832insG | I944fsX959 | M4-M5 | [5] | |
| c.3209-68A→G | Splicing | [2] | ||
| c.del Ex 22 + 23 | M5-6 | [5] | ||
| C.3537-1G→A | Splicing | [3],[5] | ||
| c.3779-91del | Q1260fsX1283 | C-terminus | [3],[5] | |
| c.del Ex 25 - 27 | C-terminus | [5] | ||
| c.del Ex 26 | Y1533X | C-terminus | No | [5] |
| c.5017-18delT | L1673fsX1675 | C-terminus | No | [5] |
| c.del Ex 31 + 32 | C-terminus | No | [5] | |
| c.5057+2T→C | Splicing | C-terminus | [5] | |
| c.A5775G | Splicing | C-terminus | [5] | |
Diagnosis
[edit]Diagnosis typically occurs during the first six months of life due to the characteristic neurological symptoms. These symptoms include muscle spasms, tetany, and seizures. Laboratory testing reveals hypomagnesemia (decreased serum magnesium levels), hypocalcemia (decreased serum calcium levels), and little to no measurable PTH levels. Diagnosis is confirmed with these symptoms and can be further solidified with genetic sequencing of the TRPM6 gene.[citation needed]
Treatment
[edit]Treatment of HSH involves administration of high doses of magnesium salts. These salts may be taken orally or otherwise (e.g. subcutaneously). This treatment works by increasing magnesium absorption through the non-TRPM6 mediated paracellular transport pathways. This treatment must be continued throughout life.[citation needed]
History
[edit]HSH was originally believed to be an X-linked disorder due to the preponderance of affected males. With the finding that mutations in TRPM6 (on chromosome 9) are causative for the disorder this is no longer the case. Of recent interest, however, is the characterization of a patient with symptoms similar to HSH who has a translocation of the chromosomes 9 and X.[7]
See also
[edit]References
[edit]- Konrad M, Schlingmann K, Gudermann T (2004). "Insights into the molecular nature of magnesium homeostasis". Am J Physiol Renal Physiol. 286 (4): F599–605. doi:10.1152/ajprenal.00312.2003. PMID 15001450.
Footnotes
[edit]- ^ "TRPM6 gene: MedlinePlus Genetics". medlineplus.gov. Retrieved 2023-07-02.
- ^ a b c d e Walder R, Landau D, Meyer P, Shalev H, Tsolia M, Borochowitz Z, Boettger M, Beck G, Englehardt R, Carmi R, Sheffield V (2002). "Mutation of TRPM6 causes familial hypomagnesemia with secondary hypocalcemia". Nat Genet. 31 (2): 171–4. doi:10.1038/ng901. PMID 12032570. S2CID 33192419.
- ^ a b c d e f g Schlingmann K, Weber S, Peters M, Niemann Nejsum L, Vitzthum H, Klingel K, Kratz M, Haddad E, Ristoff E, Dinour D, Syrrou M, Nielsen S, Sassen M, Waldegger S, Seyberth H, Konrad M (2002). "Hypomagnesemia with secondary hypocalcemia is caused by mutations in TRPM6, a new member of the TRPM gene family". Nat Genet. 31 (2): 166–70. doi:10.1038/ng889. PMID 12032568. S2CID 40990544.
- ^ Chubanov V, Waldegger S, Mederos y Schnitzler M, Vitzthum H, Sassen M, Seyberth H, Konrad M, Gudermann T (2004). "Disruption of TRPM6/TRPM7 complex formation by a mutation in the TRPM6 gene causes hypomagnesemia with secondary hypocalcemia". Proc Natl Acad Sci USA. 101 (9): 2894–9. Bibcode:2004PNAS..101.2894C. doi:10.1073/pnas.0305252101. PMC 365716. PMID 14976260.
- ^ a b c d e f g h i j k l m n o p q r s t u v w Schlingmann K, Sassen M, Weber S, Pechmann U, Kusch K, Pelken L, Lotan D, Syrrou M, Prebble J, Cole D, Metzger D, Rahman S, Tajima T, Shu S, Waldegger S, Seyberth H, Konrad M (2005). "Novel TRPM6 mutations in 21 families with primary hypomagnesemia and secondary hypocalcemia". J Am Soc Nephrol. 16 (10): 3061–9. doi:10.1681/ASN.2004110989. PMID 16107578.
- ^ Li M, Jiang J, Yue L (2006). "Functional Characterization of Homo- and Heteromeric Channel Kinases TRPM6 and TRPM7". J Gen Physiol. 127 (5): 525–37. doi:10.1085/jgp.200609502. PMC 2151519. PMID 16636202.
- ^ a b c d e f g Jalkanen R, Pronicka E, Tyynismaa H, Hanauer A, Walder R, Alitalo T (2006). "Genetic background of HSH in three Polish families and a patient with an X;9 translocation". Eur J Hum Genet. 14 (1): 55–62. doi:10.1038/sj.ejhg.5201515. PMID 16267500.
- ^ a b Voets T, Nilius B, Hoefs S, van der Kemp A, Droogmans G, Bindels R, Hoenderop J (2004). "TRPM6 forms the Mg2+ influx channel involved in intestinal and renal Mg2+ absorption". J Biol Chem. 279 (1): 19–25. doi:10.1074/jbc.M311201200. hdl:2066/57440. PMID 14576148.