Gluconate-proton symporter
| Gluconate:H+ Symporter | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | GntP | ||||||||
| Pfam | PF02447 | ||||||||
| InterPro | IPR003474 | ||||||||
| |||||||||
The gluconate:H+ symporter (GntP) family (TC# 2.A.8) is a family of transport proteins belonging to the ion transporter (IT) superfamily.[1] Members of the GntP family include known gluconate permeases of E. coli and Bacillus species such as the D-Gluconate:H+ symporter of Bacillus subtillus (GntP; TC# 2.A.8.1.1) and the D-fructuronate/D-gluconate:H+ symporter of E. coli (GntP; TC# 2.A.8.1.3).[2][3][4] A representative list of proteins belonging to the GntP family can be found in the Transporter Classification Database.[5]
Structure
[edit]Bioinformatic analysis suggests these proteins are of about 450 residues and possess 12 or 14 putative transmembrane α-helical spanners. No crystal structure data are available for GntP proteins as of early 2016.
Function
[edit]Four of the seven E. coli paralogues have been found to possess active gluconate uptake activity, and one of them (GntW; TC# 2.A.8.1.2) can accommodate both L-idonate and D-gluconate, although L-idonate is the physiological substrate. Another (GntP) transports D-gluconate with high affinity but is specifically induced by and transports D-fructuronate.[4] GntT of E. coli is the physiological gluconate permease.[6]
Transport reaction
[edit]The generalized transport reaction catalyzed by proteins of the GntP family is:
Carbohydrate acid (out) + nH+ (out) → Carbohydrate acid (in) + nH+ (in)
References
[edit]- ^ Prakash S, Cooper G, Singhi S, Saier MH (December 2003). "The ion transporter superfamily". Biochimica et Biophysica Acta (BBA) - Biomembranes. 1618 (1): 79–92. doi:10.1016/j.bbamem.2003.10.010. PMID 14643936.
- ^ Peekhaus N, Tong S, Reizer J, Saier MH, Murray E, Conway T (February 1997). "Characterization of a novel transporter family that includes multiple Escherichia coli gluconate transporters and their homologues". FEMS Microbiology Letters. 147 (2): 233–8. doi:10.1016/s0378-1097(96)00532-0. PMID 9119199.
- ^ Reizer A, Deutscher J, Saier MH, Reizer J (May 1991). "Analysis of the gluconate (gnt) operon of Bacillus subtilis". Molecular Microbiology. 5 (5): 1081–9. doi:10.1111/j.1365-2958.1991.tb01880.x. PMID 1659648. S2CID 2006623.
- ^ a b Bates Utz C, Nguyen AB, Smalley DJ, Anderson AB, Conway T (November 2004). "GntP is the Escherichia coli Fructuronic acid transporter and belongs to the UxuR regulon". Journal of Bacteriology. 186 (22): 7690–6. doi:10.1128/JB.186.22.7690-7696.2004. PMC 524916. PMID 15516583.
- ^ "2.A.8 The Gluconate:H+ Symporter (GntP) Family". Transporter Classification Database. Retrieved 2016-03-04.
- ^ Porco A, Peekhaus N, Bausch C, Tong S, Isturiz T, Conway T (March 1997). "Molecular genetic characterization of the Escherichia coli gntT gene of GntI, the main system for gluconate metabolism". Journal of Bacteriology. 179 (5): 1584–90. doi:10.1128/jb.179.5.1584-1590.1997. PMC 178870. PMID 9045817.
As of 9 March 2016, this article is derived in whole or in part from Transporter Classification Database. The copyright holder has licensed the content in a manner that permits reuse under CC BY-SA 3.0 and GFDL. All relevant terms must be followed. The original text was at "The Gluconate:H+ Symporter (GntP) Family"
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