DescriptionSample of White-Tailed Deer Sperm Under Fluorescent Microscopy.jpg
English: This photograph is of a sample of epididymal white-tailed deer spermatozoa that was stained with PNA (peanut agglutinin) and viewed under fluorescent microscopy at 400x magnification. The acrosome (the “cap” over the rest of the sperm head) is stained green, while the rest of the head, including the nucleus is stained purple or pink, and the tail is stained red. The brighter the stain, the more capable that particular spermatozoan is at fertilizing an oocyte.
This photograph is part of an ongoing undergraduate research project that seeks to test the fertilization capabilities of epididymal spermatozoa collected from recently deceased white-tailed bucks. All sperm samples were stained with peanut agglutinin and were compared to ejaculated sperm from the same species to see if there are any major differences between the epididymal and ejaculated samples. My team and I are hoping that this project will lead to improved reproductive efficiency in cervid species by demonstrating that deceased bucks are still capable of producing viable offspring.
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