Dos Hermanas Club de Fútbol was a Spanish football team based in Dos Hermanas, in the autonomous community of Andalusia. Founded in 1971 and dissolved in 2014, it last played in Regional Preferente de Sevilla, holding home games at Estadio Miguel Román García, with a capacity of 3,200 seats.
Originally known as Dos Hermanas CF, the club was dissolved in 2014. After that, two distinct successor teams were created. In 2014, Atlético Dos Hermanas CF, and in 2021, Dos Hermanas CF 1971. Both of them are currently competing on the Regional leagues.