Dische test
The Dische test, or Dische reaction, is used to distinguish DNA from RNA. It was invented by Zacharias Dische.
Method
[edit]Dische's diphenylamine reagent consists of diphenylamine, glacial acetic acid, sulfuric acid, and ethanol.[1]
When heated with DNA, it turns blue in the presence of DNA. A more intense blue color indicates a greater concentration of DNA.
Mechanism
[edit]The acid converts deoxyribose to a molecule that binds with diphenylamine to form a blue substance. The reagent does not interact with RNA, so can be used to distinguish DNA from RNA.[2][3]
See also
[edit]References
[edit]- ^ Patterson, J; Mura, C (4 February 2013). "Rapid colorimetric assays to qualitatively distinguish RNA and DNA in biomolecular samples". Journal of Visualized Experiments (72): e50225. doi:10.3791/50225. PMC 3597041. PMID 23407542.
- ^ "Nucleic Acids: DNA extraction and Dische's Diphenylamine test (Activity) | Biology OER". Retrieved 8 March 2023.
- ^ "DNA-Dische Diphenylamine Determination". biocyclopedia.com. Retrieved 8 March 2023.