Crenobacter cavernea
Crenobacter cavernea Cave-375 is a gram-negative bacterium that is closely related to a previously discovered Crenobacter cavernae strain K1W11S-77ͭ. C. cavernea Cave-375 has not directly been described morphologically, however the related strain K1W11S-77ͭ is a "rod-shaped, motile, and strictly aerobic novel bacteria".[1]
Its metabolism has not yet been determined.
C. cavernea Cave-375 was first identified from a water sample coming from a dripping stalactite. This stalactite was located in the Algar do Pena cave in the karst Estremadura Limestone Massif in central western Portugal.[2]
C. cavernea Cave-375 was first isolated and "grown on nutrient agar at 25 degrees Celsius".[2]
Its ecology is not yet known. With the sequencing of the genome of C. cavernea Cave-375, the ecological impact should be able to be identified.[2]
Diversity
[edit]C. cavernea Cave-375 belongs in the Proteobacteria phylum, Neisseriaceae family, and Crenobacter cavernea species. By comparing the 16s rRNA of the CAVE-375 stain to Crenobacter cavernea species, a 99% similarity value was calculated. When comparing DNA-DNA hybridization using a Genome-to-Genome Distance Calculator, a 62.66% hybridization percentage was found.[2]
Genome
[edit]"Genomic DNA was extracted from C. cavernea Cave-375 using an NZY microbial gDNA isolation kit (NZYTech, Portugal)".[2] The whole genome was then sequenced using whole genome shotgun sequencing method. With this, "17,325,372 high-quality raw sequences were assembled into 15 contigs with an N50 value of 323,281 and a total genome size of 2,273,143 base pairs (2.9 Mb)".[2] NCBI Prokaryotic Genome Annotation Pipeline was able to identify a 65.9% GC content and sequencing coding for proteins and tRNA. "2,779 protein coding sequences and 63 tRNA sequences" were identified using this method.[2]
References
[edit]- ^ Zhu, Hai-Zhen; Jiang, Cheng-Ying; Liu, Shuang-Jiang (2019). "Crenobacter cavernae sp. nov., isolated from a karst cave, and emended description of the genus Crenobacter". International Journal of Systematic and Evolutionary Microbiology. 69 (2): 476–480. doi:10.1099/ijsem.0.003179. PMID 30556805.
- ^ a b c d e f g Coelho, Catarina; Veríssimo, António; Tiago, Igor (2019). "High-Quality Draft Genome Sequences of Crenobacter cavernae Strain CAVE-375 and Oxalobacteriaceae sp. Strain CAVE-383, Two Bacteria Isolated from Dripping Water in a Karstic Cave in Portugal". Microbiology Resource Announcements. 8 (12): e00127-19. doi:10.1128/MRA.00127-19. PMC 6430319. PMID 30938702.